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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMH-PBR1X
$49.50
DCP-MEMH-PBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains 25 mM HEPES Contains Calcium Contains Magnesium Contains Low Glucose (1000 mg/L) Without Sodium Bicarbonate Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-MEMH-PBR1X is a Microfluidics Suitable, ultra-filtered MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for primary fibroblasts and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides robust CO2-independent pH buffering. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] 25mM HEPES | [-] Sodium Pyruvate, [-] Sodium Bicarbonate, [-] Phenol Red.

  • Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high-glucose stress
  • NEAA pre-loaded — reduces metabolic burden and ammonia accumulation from de novo amino acid synthesis
  • 25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO2; suited to open-air handling, flow cytometry prep, and atmospheric incubation
  • 0.04 µm final filtration — sub-mycoplasma purity; endotoxin release specification < 0.05 EU/mL for sensitive primary and hematopoietic cell cultures
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Ultrapure Type 1 water (18.2 MΩ·cm), manufactured under an ISO 13485:2016 QMS, ISO Class 5 aseptic fill
DCP-MEMH-PBR1X Sizes: 500 mL, 1000 mL Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] L-Glutamine, [+] 25mM HEPES
    [-] Sodium Pyruvate, [-] Sodium Bicarbonate, [-] Phenol Red
  • AppearancePale yellow-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235–275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a mainstay of primary cell biology — but conventional 0.22 µm filtered MEM passes mycoplasma, particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ reduces these risk factors while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> particulate compliance. Low-particulate primary cell media helps prevent chip microchannel clogging.

target

Total metabolic control

Low Glucose (1.0 g/L) plus pre-loaded NEAA gives researchers a defined, physiological carbon and nitrogen backdrop suited to primary cells sensitive to high-glucose or high-ammonia stress.

water_drop

Ultrapure-grade water

Formulated with Type 1 water (18.2 MΩ·cm) to reduce trace-metal and organic contaminants across every batch.

visibility

Low background for imaging

0.04 µm final filtration reduces particulate baseline for confocal microscopy and biosensor/TEER-monitored chips; the phenol red–free formulation avoids absorbance interference in the visible range.

science

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA is a long-established base for primary fibroblasts, neurons, epithelial and vascular cells, now delivered at 0.04 µm purity.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, alternating dedicated 0.1 µm prefilters with 0.04 µm final filters, reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate and protein aggregates; the 0.1 µm absolute rating also provides mycoplasma-retentive filtration (mycoplasma range 0.2–0.3 µm), protecting the downstream 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter, establishing the first sub-micron purity baseline.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated 0.1 µm prefilter — again mycoplasma-retentive — protecting the second 0.04 µm cartridge from fouling.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under validated ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma control via 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEMH-PBR1X Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid ? Quadruple-stage filtration system: 0.1?μm Prefiltration I, 0.04?μm Final filtration I, 0.1?μm Prefiltration II, 0.04?μm Final filtration II Polish ? Microfluidics Suitable MEM Low Glucose + NEAA + 25mM HEPES for organ-on-a-chip (OoC) and microfluidic cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-PBR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-PBR1X is purpose-built for primary fibroblasts and related primary cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s Salts ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate, phenol red–free formulation reduces optical interference for confocal microscopy, TEER sensors, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] 25mM HEPES | [-] Sodium Pyruvate, [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow-colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 235–275 mOsm/kg H2O
Total ingredients 35
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release spec — see §Manufacturing)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Available pack sizes 500 mL, 1000 mL
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 35 ingredients verified per lot with CAS numbers, organized across three composition tabs (Inorganic Salts; Amino Acids; Vitamins & Others).

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
i-Inositol 87-89-8 2.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-PBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water significantly reduces trace-metal and organic (TOC) contaminants.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis generated for every lot.

Endotoxin — USP <85> BET

Release specification < 0.05 EU/mL; see batch-level quality control note below.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 235–275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMH-PBR1X compares

FluxMPS™ DCP-MEMH-PBR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH-PBR1X (FluxMPS™) Conventional MEM Low Glucose (0.22 µm filtered) Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Not applicable Not applicable
MEM + NEAA + HEPES-only CO2-free base — no pyruvate, no phenol red, with L-Glutamine check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle 0.1 µm mycoplasma-retentive cancel No cancel No
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-PBR1X.

Yes. DCP-MEMH-PBR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with sub-mycoplasma filtration purity is particularly suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.
 
HEPES-only CO2-independent buffering removes the need for sodium bicarbonate; excluding phenol red removes optical/absorbance interference for imaging and biosensor assays; sodium pyruvate is left out so researchers can add their own defined secondary carbon source (for example, 1 mM sodium pyruvate) matched to their cell model. NEAA is pre-loaded to reduce the biosynthetic burden on primary cells that would otherwise synthesize these amino acids de novo from glutamine and glucose, improving viability in low-serum conditions and reducing ammonia byproduct accumulation.
No. This formulation is CO2-independent: HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation, making it suitable for open-bench handling and instruments without CO2 control.
Yes. When adding serum, growth factors, or other protein-containing supplements, pre-filter through a 0.2 µm low-protein-binding PES or PVDF membrane to preserve the medium's low-particulate, low-bioburden profile; 0.04 µm membranes are not recommended for protein-containing additions, as they can strip serum components such as IgM, VLDL and lipoproteins and clog rapidly. For defined, protein-free additions (e.g., small-molecule inhibitors), a 0.1 µm filter is generally suitable.
FluxMPS™ DCP-MEMH-PBR1X is released to meet < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification. For primary cells, endotoxin can activate TLR4 signaling, altering fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier integrity — control at this level reduces that confound.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1, light obscuration), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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