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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-MEMH-PB1X
$49.50
DCP-MEMH-PB1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Glucose (Low, 1.0 g/L) Without Sodium Bicarbonate Without Sodium Pyruvate

FluxMPS™ DCP-MEMH-PB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA + 25mM HEPES formulation built for primary fibroblasts and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides CO2-independent pH buffering. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] 25mM HEPES, [+] Phenol Red | [-] Sodium Pyruvate, [-] Sodium Bicarbonate.

  • Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high-glucose osmotic and metabolic stress
  • NEAA pre-loaded — reduces de novo synthesis burden and ammonia accumulation in primary fibroblasts, neurons, and epithelial cells
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO2-independent pH stability for open-air handling, flow cytometry prep, and atmospheric incubation
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final cut-off
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Ultrapure Type 1 water (18.2 MΩ·cm) and ISO Class 5 aseptic fill under an ISO 13485:2016 quality system
  • Sodium pyruvate and sodium bicarbonate excluded — add pyruvate or a defined carbon source at the concentration your protocol requires
  • Custom pH, glucose, and HEPES concentration modifications available on request
DCP-MEMH-PB1X Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (Low, 1.0 g/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate
  • AppearanceOrange-to-red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235–275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes particulates, mycoplasma-sized organisms, and endotoxin that can alter primary cell phenotype. FluxMPS™ addresses these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance. Particulate-controlled primary cell media helps reduce the risk of chip channel clogging.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: CO2-stable pH

25 mM HEPES helps maintain pH stability during open-air suspension handling, flow cytometry prep, and multi-well assay setup outside CO2 incubators.

shield

Low endotoxin release specification

< 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch. Lower endotoxin loads are relevant to endotoxin-sensitive primary and hematopoietic cultures, where elevated endotoxin can confound activation and inflammatory signaling assays.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a 0.04 µm final polish under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I — Large Particulate Removal

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a standard 0.22 µm filter (not tested per lot).

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.1 µm/0.04 µm barrier filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS(TM) DCP-MEMH-PB1X Minimum Essential Medium (MEM), Low Glucose, NEAA and 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM Low Glucose + NEAA + 25mM HEPES for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-PB1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-PB1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant of this formulation is available on request for automated bioreactor and robotic handling systems — see the Grade note above for the distinction from this Microfluidics Suitable (0.04 µm) product.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s Salts ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps reduce particulate clogging risk in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate filtration reduces background interference for confocal imaging, TEER sensors, and biosensor applications on primary cell chips. Note: this formulation contains phenol red; phenol-red-free variants are available on request for applications requiring reduced autofluorescence.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (Low, 1.0 g/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate
Appearance Orange-to-red colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 235–275 mOsm/kg H2O
Total ingredients 36 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others), organized in 3 tabs
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Available sizes: 500 mL, 1000 mL.
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 36 ingredients verified per lot with CAS numbers. NEAA components are listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 11.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-PB1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — controls trace metal and organic carbon (TOC) contamination during formulation.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm) — light obscuration.

Osmolality — USP <785>

Target: 235–275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-MEMH-PB1X lot at support@diagnocine.com.
Product Comparison

How DCP-MEMH-PB1X compares

FluxMPS™ DCP-MEMH-PB1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH-PB1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Standard grade (0.22 µm filtered) Standard grade (0.22 µm filtered)
MEM + NEAA + HEPES-only, CO2-free formulation (no pyruvate; L-Glutamine included) check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.04 µm stage) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
USP <788> particulate tested (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes — Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-PB1X.

Yes. DCP-MEMH-PB1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final cut-off, delivering low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with 0.04 µm filtration purity is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.
 
HEPES alone provides CO2-independent pH buffering; sodium pyruvate is left out so it can be added fresh at the concentration your protocol requires. L-Glutamine is already included. This gives a CO2-independent primary cell base where only the secondary carbon source needs to be added — useful for pyruvate dose-response experiments on open-top chips. Add pyruvate using a 0.2 µm filter if preparing a stock solution.
No. This formulation is CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation.
Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — the 0.04 µm final filter used in this product's own manufacturing is not appropriate for serum, as it would retain immunoglobulins, lipoproteins, and growth factors. Defined, protein-free additions may use a 0.1 µm filter. NEAA is already pre-loaded, so no additional NEAA supplementation is needed.
Each manufacturing batch is released against a specification of less than 0.05 EU/mL by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL). This is a batch-level release specification, not a per-unit test result. Low endotoxin is relevant for primary cells, where endotoxin can confound fibroblast activation, neuronal gene expression, and epithelial barrier formation.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma barrier filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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