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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A low-glucose MEM base buffered with 25 mM HEPES, formulated without sodium pyruvate and without phenol red, processed through a validated four-pass sterile filtration train reaching a 0.04 µm final cut-off — making it microchannel-safe from day one.
- Quadruple-stage filtration train: 0.1 µm ×2 + 0.04 µm ×2 (four sterile passes)
- Low-glucose MEM base (1000 mg/L D-Glucose) with 25 mM HEPES buffering
- Formulated without sodium pyruvate and without phenol red — confirm supplementation needs before use
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP Chapter 85 BET)
- pH 7.4 (USP Chapter 791); HEPES-buffered, standard 5–10% CO₂ incubation remains compatible
- Manufactured under an ISO 13485:2016 quality management system; final packaging and testing at Diagnocine, Totowa, NJ
- Mycoplasma risk mitigated by 0.1 µm mycoplasma-retentive filtration (not tested per lot)
- Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
- Glucose1000 mg/L (1.0 g/L)
- L-Glutamine292 mg/L
- Sodium PyruvateNot added / None
- HEPES5958 mg/L (25 mM)
- pH (USP <791>)7.4
- Osmolality (USP <785>)See CoA
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage, Sterile)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and inconsistent endotoxin loads that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is built to address these failure modes at the filtration stage.
Microchannel-safe purity
0.04 µm final filter retains particles down to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.
Total metabolic control
Defined low-glucose carbon source and precise nutrient concentrations support metabolic flux experiments and Warburg-pathway studies.
Ultrapure-grade water
Prepared with Type 1 water (18.2 MΩ·cm resistivity), supporting low trace-metal and organic-carbon (TOC) background in the finished medium.
Low background for imaging
Quadruple-stage filtration keeps particulate baseline low, supporting confocal microscopy and live-cell biosensor work. Riboflavin, a native medium component, contributes native fluorescence to consider when designing fluorescence-based assays.
Rich, stable nutrient profile
Micro-batch precision locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.
Customization on demand
pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid is processed through a four-pass filtration train — two dedicated prefilter/final-filter pairs — reaching a 0.04 µm final cut-off, well beyond the 0.22 µm standard used across the industry.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter cannot capture.
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3
0.1 µm Prefiltration II
A second, dedicated prefilter protecting the second 0.04 µm cartridge — redundancy against carryover from the first pass.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish under ISO Class 5 laminar-flow conditions.
Performance vs. conventional media
Two prefilter/final-filter pairs in series reaching a 0.04 µm final cut-off provide a meaningfully cleaner particulate baseline than single-pass 0.22 µm filtration, with USP <788> Method 1 (light obscuration) particulate compliance verified on every lot.
© Diagnocine® — DCP-MEMH-PRN1X
Designed for next-generation cell culture platforms
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid is validated for organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate load and endotoxin variability matter.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion, beyond the 0.04 µm Microfluidics Suitable cut-off described above.
- Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
- Valve & Sensor Protection: reduces particulate-induced blockage risk in precision fluidic systems
- Extended Perfusion Stability: supports flow rate consistency across multi-week automated runs
Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
Quadruple-stage filtered formulation helps reduce microchannel clogging risk and supports laminar flow integrity.
Warburg Effect & Metabolic Research
Defined low-glucose carbon source supports metabolic flux analysis and Warburg-pathway studies.
iPSC-Derived Models
Ultra-filtered formulation supports sensitive iPSC differentiation protocols where particulate and endotoxin variability can cause off-target effects.
Endothelial & Primary Cells
Microchannel-safe purity supports endothelial barrier integrity assays, including TEER measurements.
Metabolic Flux Analysis
Defined nutrient base supports isotope-tracing workflows; note that this formulation contains baseline glucose (1000 mg/L) and L-glutamine (292 mg/L), which should be accounted for in tracer study design. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Low particulate baseline supports confocal imaging and biosensor integration workflows.
Lot-release quality parameters
Every production lot of FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid undergoes the quality-release battery below before shipment. Available pack sizes: 500 mL, 1000 mL.
| Parameter | Specification |
|---|---|
| Formulation | MEM base + L-Glutamine, Sodium Bicarbonate, HEPES, Calcium, Magnesium, Glucose; without Phenol Red, without Sodium Pyruvate |
| Appearance | Pale-yellow colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | See CoA |
| Glucose | 1000 mg/L (1.0 g/L) |
| L-Glutamine | 292 mg/L |
| Sodium Pyruvate | Not added / None |
| Phenol Red | Not added / None |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch-level release specification; see §Manufacturing & QA) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | Compliant |
| Particulate ≥25 µm USP <788> Method 1 | Compliant |
| Water Purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. ISO 13485 | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Not recommended |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | HEPES-buffered; reduced CO₂ dependency — standard 5–10% CO₂ incubation remains compatible |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot documentation, CoA available |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch precision manufacturing |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
Complete formulation with CAS numbers, reproduced from the manufacturer specification. Total: 29 components. Custom compositions available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2200.000 |
| Sodium chloride | 7647-14-5 | 6800.00 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Cystine dihydrochloride | 30189-89-0 | 31.300 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 51.900 |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Niacinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| i-Inositol | 87-89-8 | 2.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| HEPES | 7365-45-9 | 5958.000 |
ISO 13485:2016 manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system, with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering manufacturing, testing, and release for every production lot.
Ultrapure Type 1 Water
Media prepared with 18.2 MΩ·cm resistivity water for low trace-metal and organic-carbon background.
ISO Class 5 Fill & Finish
Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.
Micro-Batch Precision
Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay per batch. Release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.
Osmolality — USP <785>
Freezing-point osmometry per USP <785>. Result: See CoA.
Documentation — CoA & Full Lot Records
Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.
How DCP-MEMH-PRN1X (FluxMPS™) compares
Side-by-side comparison against conventional 0.22 µm-filtered alternatives of a similar base formulation.
| Parameter | DCP-MEMH-PRN1X (FluxMPS™) | Conventional MEM (0.22 µm) | Standard MEM alternative |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Base Formulation | Low Glucose & 25mM HEPES, w/o Sodium Pyruvate, w/o Phenol Red | MEM Standard | MEM Equivalent |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 passes | 1 pass | 1–2 passes |
| Mycoplasma-retentive filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Variable | Variable |
| Microfluidic channel compatibility | check_circle Validated | cancel Elevated clogging risk | cancel Elevated clogging risk |
| Custom formulation | check_circle On request | cancel | Limited |
Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Minimum Essential Medium (MEM), Low Glucose & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.
Supporting literature
Peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media and microfluidic cell culture applications.
- Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
- Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
- Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
- Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
- Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
- Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
- Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
- Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
