Malate Dehydrogenase(decarboxylating)
Product#: FNK-MDH-73-01
$524.51
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Malate Dehydrogenase(decarboxylating)
Cat. No.: FNK-MDH-73-01
Size: 1ml
Storage: -20°C
Specifications
Code #: MDH-73-01
Form: 50 mM Tris-HCl (pH 8.0), 50 mM NaCl
Description:
This thermostable enzyme obtained from hyper-thermophilic microorganisms have some unique characteristics such as highly stable and durable for long term use.
Activity Measurement:
Reaction mix (50 mM PIPES-KOH (pH 6.5), 10 mM Pyruvic acid, 0.3 mM NADH, 10 mM NaHCO3, 10 mM MgCl2 and appropriate amount of the enzyme) was incubated at 37℃ and A340 was monitored. One unit is defined as the amount of the enzyme oxidizing 1 μmol of NADH (ε340=6.22 mM-1 cm-1 ) per 1 minute using Pyruvic acid as a substrate.
pH profile:
Thermostability:
Effect of metal ions on the activity:
(a) Divalent metal ion specificity
(b) Concentration dependence
Kinetic parameters:
(a) Carboxylation reaction (Km @ 37℃, pH 6.5) Pyruvic acid: 0.89 mM, NaHCO3: 0.83 mM, NADH: 0.042 mM
(b) Decarboxylation reaction (Km @ 37℃, pH 9.5) L-Malic acid: 0.14 mM, D-Malic acid: 3.6 mM, NAD+ : 0.12 mM
| For research use only, not for diagnostic use |
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