FluxMPS™ MCDB 302 Medium w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ MCDB 302 Medium w/o Sodium Bicarbonate: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Approximately 5× fewer particles ≥10 µm than conventional 0.22 µm-filtered media.
- Quadruple-stage 0.1 µm ×2 + 0.04 µm ×2 filtration train reaching a 0.04 µm final cut-off — Microfluidics Suitable for organ-on-a-chip and MPS perfusion
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- Bicarbonate-free formulation with 1.8 g/L D-glucose, 438.6 mg/L L-glutamine, and 110 mg/L sodium pyruvate — buffer system must be supplemented by the user (see FAQ)
- Rich trace-element profile including molybdate, vanadate, selenite, and manganese salts alongside standard inorganic salts
- Manufactured under an ISO 13485:2016 quality management system; final QC and customization at Diagnocine, Totowa, NJ
- 49 formulation components released per lot: 12 inorganic salts, 20 amino acids, 9 vitamins, and 8 other components
- Custom pH, glucose, HEPES, salts & nutrients available on request — support@diagnocine.com
- Glucose1801.6 mg/L ([+])
- L-Glutamine438.6 mg/L ([+])
- Sodium Pyruvate110 mg/L ([+])
- pH (USP <791>)7.4
- Osmolality (USP <785>)300–340 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (typically 0.2–0.3 µm in diameter), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.
Microchannel-safe purity
0.04 µm final filtration protects microchannel geometries from sub-visible particulates and aggregates that a standard 0.22 µm filter cannot retain.
Total metabolic control
A fully defined glucose (1.8 g/L), L-glutamine, and sodium pyruvate profile supports Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under trace-metal and organic-carbon (TOC) controlled conditions.
Low background for imaging
Low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — engineered to retain particulates, aggregates, and mycoplasma-scale contaminants that pass through conventional 0.22 µm filtration unchecked.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter and preserves microchannel chip geometries downstream.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains fine particulates and sub-micron bioburden that a standard 0.22 µm filter allows through.
-
3
0.1 µm Prefiltration II
A second, dedicated prefilter protecting the second 0.04 µm cartridge — giving the train full redundancy rather than acting as a descending cascade.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Performance vs. conventional media
By USP <788> Method 1 (light obscuration), FluxMPS™ delivers approximately 5× fewer particles ≥10 µm than standard 0.22 µm-filtered media — the difference between successful long-duration perfusion and channel occlusion within 48 hours.
© Diagnocine® — DCP-M302-B1X
Optimized for next-generation cell biology platforms
FluxMPS™ MCDB 302 Medium w/o Sodium Bicarbonate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.
Automated Bioreactors & Robotics
For fully automated, closed-loop bioreactor and robotic liquid-handling systems, Diagnocine also offers a 0.01 µm (10 nm) ultra nano-filtered variant of this formulation — the MPS Grade tier of the FluxMPS™ line, filtered through a six-stage cascade (0.1 µm ×2 → 0.04 µm ×2 → 0.02 µm → 0.01 µm) beyond the Microfluidics Suitable 0.04 µm cut-off described on this page.
- Total Particulate Exclusion — sub-40 nm final cut-off for zero-tolerance particulate environments
- Valve & Sensor Protection — minimizes fouling of precision microvalves and inline optical sensors
- Extended Perfusion Stability — supports multi-week closed-loop bioreactor runs without filter replacement
Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to discuss availability and lead time.
Micro Physiological System (MPS) & Chip
Ultra-low particulate, mycoplasma-retentive filtered media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Defined glucose (1.8 g/L) formulation supports Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-free perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.
Microscopy & Optical Sensing
Low particulate baseline supports confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Full technical specification
Every lot of FluxMPS™ MCDB 302 Medium w/o Sodium Bicarbonate: 1X Liquid is released against comprehensive multi-parameter QC specifications.
Available pack sizes: 500 mL, 1000 mL.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, Phenol Red, Calcium, Magnesium, Glucose, Sodium Pyruvate / [-] Sodium Bicarbonate |
| Appearance | Clear solution; light pink to red hue from phenol red (1.242 mg/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 300–340 mOsm/kg H2O |
| Glucose | 1801.6 mg/L ([+]) |
| L-Glutamine | 438.6 mg/L ([+]) |
| Sodium Pyruvate | 110 mg/L ([+]) |
| Phenol Red | 1.242 mg/L ([+]) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL (release specification) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO2 requirement | Bicarbonate-free and HEPES-free as supplied; supplement sodium bicarbonate to match your incubator's CO2 level, or add 10–25 mM HEPES for CO2-independent culture |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 49 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| Ammonium metavanadate | 7803-55-6 | 0.00117 |
| Calcium chloride dihydrate | 10035-04-8 | 88.210 |
| Cupric sulfate pentahydrate | 7758-99-8 | 0.0025 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 141.980 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride hexahydrate | 7791-18-6 | 122.000 |
| Manganese sulfate | 7785-87-7 | 0.000151 |
| Molybdic acid ammonium tetrahydrate | 12054-85-2 | 0.0124 |
| Potassium chloride | 7447-40-7 | 223.650 |
| Sodium chloride | 7647-14-5 | 7599.000 |
| Sodium selenite | 10102-18-8 | 0.00173 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.863 |
| Component | CAS Number | mg/L |
|---|---|---|
| Glycine | 56-40-6 | 7.510 |
| L-Alanine | 56-41-7 | 8.910 |
| L-Arginine hydrochloride | 1119-34-2 | 210.700 |
| L-Asparagine monohydrate | 5794-13-8 | 15.000 |
| L-Aspartic acid | 56-84-8 | 13.310 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 17.560 |
| L-Glutamic acid | 56-86-0 | 14.710 |
| L-Glutamine | 56-85-9 | 438.600 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.970 |
| L-Isoleucine | 73-32-5 | 3.940 |
| L-Leucine | 61-90-5 | 13.120 |
| L-Lysine hydrochloride | 657-27-2 | 36.540 |
| L-Methionine | 63-68-3 | 4.480 |
| L-Phenylalanine | 63-91-2 | 4.960 |
| L-Proline | 147-85-3 | 34.530 |
| L-Serine | 56-45-1 | 10.510 |
| L-Threonine | 72-19-5 | 11.910 |
| L-Tryptophan | 73-22-3 | 2.040 |
| L-Tyrosine disodium salt dihydrate | 7.896 | |
| L-Valine | 72-18-4 | 11.720 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 13.960 |
| D-Biotin | 58-85-5 | 0.00733 |
| D-Ca-Pantothenate | 137-08-6 | 0.238 |
| Folic acid | 59-30-3 | 1.324 |
| Niacinamide | 98-92-0 | 0.0366 |
| Pyridoxine hydrochloride | 58-56-0 | 0.0617 |
| Riboflavin | 83-88-5 | 0.0376 |
| Thiamine hydrochloride | 67-03-8 | 0.337 |
| Vitamin B12 | 68-19-9 | 0.136 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1801.600 |
| Hypoxanthine | 68-94-0 | 4.083 |
| Linoleic acid | 60-33-3 | 0.0841 |
| myo-Inositol | 87-89-8 | 18.020 |
| Phenol red sodium salt | 34487-61-1 | 1.242 |
| Putrescine dihydrochloride | 333-93-7 | 0.161 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| Thioctic acid | 62-46-4 | 0.206 |
Manufacturing & compliance
Every batch is subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA, and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, low TOC, produced under trace-metal and organic-carbon controlled conditions.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves filtration gains through to the final container.
Micro-Batch Precision
Small-batch production with tight osmolality (±5 mOsm/kg) and pH (±0.05) process control — critical for reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL.
Sterility — USP <71>
14-day membrane filtration sterility test; no growth observed at release.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 300–340 mOsm/kg H2O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance, and clarity
- Sterility
How DCP-M302-B1X compares
Critical differences in grade, filtration, mycoplasma barrier, and endotoxin release specification versus conventional 0.22 µm-filtered media.
| Parameter | DCP-M302-B1X (FluxMPS™) | Conventional MCDB 302 (0.22 µm) | Standard Alternative MCDB 302 (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Formulation | [+] L-Glutamine, Phenol Red, Calcium, Magnesium, Glucose, Sodium Pyruvate / [-] Sodium Bicarbonate | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Not specified | Not specified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ MCDB 302 Medium w/o Sodium Bicarbonate: 1X Liquid (DCP-M302-B1X).
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
