LiveReceptor GABAAR (GABAAR Labeling Reagent)
LiveReceptor GABAAR (GABAAR Labeling Reagent),
Cat NO. FNK-FDV-0018B
Size 10 ug
Storage -20℃
Maker FNA
Description
Specific labeling regent for ion channel type GABA receptor Live Receptor GABAAR
LiveReceptor is the world-first reagent series of target-specific receptor labeling.
Now, we announce the new product targeting for ion channel type GABA receptors (GABAARs)
LiveReceptor GABAAR is a powerful tool to investigate physiological functions of GABAAR by monitoring receptor trafficking of GABAAR in live cells. No complicated protocol is required.
Our "LiveReceptors" generally have three domains, a receptor specific ligand, fluorescein dye and acyl imidazole.
Once ligand on the LiveReceptor binds to the target receptor, acyl imidazole is activated and attacked by nucleophilic amino acids on the receptors' surface.
In this result, the receptors are directly labeled with fluorescein even after the ligand left from binding pocket. Receptor functions are still kept after labeling.
This product is commercialized by the research result of Professor Hamachi, at Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University.
Features
Chemical Information
• Formulation : C55H51N7O13
• Molecular weight : 1017.35 g/mol
• Visibility : Orange lyophilized powder
• Solubility : Soluble in DMSO
*This compound has high water-solubility, and it can be easily degraded in water and culture medium.
Please avoid storing in water.
• Excitation/ Emission: 495/515 nm *Compatible with FITC filter
Application
• Live cell imaging
• Immunocytochemistry with specific antibodies
• Immunoprecipitation with anti-fluorescein antibody
• Immunoblotting with anti-fluorescein antibody
• Drug screening for competitive GABAAR antagonist (GABA-site)
Example Data
Fig.1 Live cell imaging of labelled GABAARs in GABAAR-expressed HEK293 GABAAR (α1/β3/γ2)-expressed HEK293 cells were treated with 1 μM of LiveReceptor GABAAR in the absence or presence of 100 μM gabazine, a GABAAR selective inhibitor, for three hours at 37C and washed out three times with the basal medium. (scalebars, 20 μm)
Fig.2 Validation of GABAAR labeling by western blotting GABAAR (α1/β3/γ2)-expressed HEK293 cells were treated with 0.01-1 μM of LiveReceptor GABAAR in the absence or presence of 100 μM gabazine, a GABAAR selective inhibitor,for three hours at 37C. After wash cells, the cells were lysed and proteins were applied into SDS-PAGE and western blotting using anti-fluorescein (FL) antibody or anti-GABAARα1 subunit.
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Product Name |
Size |
Code |
Storage |
Maker |
|
|
LiveReceptor GABAAR <GABAAR Labeling Reagent> |
10 ug |
FDV-0018B |
-20℃ |
FNA |
$600 |
