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FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 2X Liquid

Product#: DCP-LL15-QPR2X
$99.00
DCP-LL15-QPR2X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 2X Liquid

Contains Calcium Contains Magnesium Without L-Glutamine Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-LL15-QPR2X is a Microfluidics Suitable, Quadruple-stage ultra-filtered Leibovitz's L-15 2X formulation using D-Galactose (1.8 g/L) and high amino-acid buffering for CO₂-independent pH maintenance — built for primary cell culture, live-cell imaging, and organ-on-a-chip (OoC) loading outside a CO₂ incubator. Processed through a four-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off, well below the 0.2–0.3 µm mycoplasma size range. Formulated without L-Glutamine, Sodium Pyruvate, and Phenol Red for fully defined, researcher-controlled supplementation.

  • CO₂-free by design: D-Galactose (1.8 g/L) energy source plus high amino-acid buffering maintains pH under atmospheric conditions — no CO₂ incubator required
  • Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaches a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL by LAL assay (USP <85>), controlled per manufacturing batch
  • Formulated without L-Glutamine, Sodium Pyruvate, and Phenol Red for fully defined, researcher-controlled supplementation
  • 2X concentrated Leibovitz's L-15 base — dilute to 1X or use directly per application-specific protocol
  • Manufactured under an ISO 13485:2016 quality management system with aseptic ISO Class 5 fill & finish
  • Osmolality 620–680 mOsm/kg H₂O and pH 7.4, consistent with the 2X concentration factor
  • Custom pH, salts, and nutrient adjustments available on request
CAT. NO.
DCP-LL15-QPR2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 — 2X Liquid
  • Media familyLeibovitz's L-15 2X
  • Carbon sourceD-Galactose — 1800 mg/L (1.8 g/L)
  • Formulation[+] Calcium, [+] Magnesium | [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate
  • AppearancePale yellow, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)620–680 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's L-15 passes mycoplasma-size particles and subvisible particulates that interfere with open-atmosphere pH stability and live-imaging clarity. FluxMPS™ is built to reduce those failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance — clean L-15 for microfluidic channel loading and clear live imaging.

science

CO₂-free by design formulation

Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source, together with high amino-acid concentrations, for CO₂-free pH buffering. This is a 2X concentrated base.

water_drop

Ultrapure-grade water

18.2 MΩ·cm Type 1 water with controlled trace-metal and total organic carbon (TOC) levels supports consistent, reproducible L-15 formulation batch to batch.

shield

Low endotoxin release specification

< 0.05 EU/mL by LAL assay (USP <85>) — relevant where endotoxin-driven TLR4 activation can confound primary cell phenotype.

bolt

Open-system flexibility

Galactose plus amino-acid buffering supports extended bench-top work, live imaging, and transport in ambient atmosphere without CO₂ supplementation.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — a repeated prefilter-plus-final-filter pair, run twice — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains particulate in the 0.2–0.3 µm mycoplasma size range as a filtration-based risk mitigation step; not a substitute for a per-lot mycoplasma test.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish in a validated ISO Class 5 (Class 100) environment.

Performance vs. conventional media

FluxMPS™ DCP-LL15-QPR2X is filtered through four sequential stages reaching a 0.04 µm final cut-off, compared to a single 0.22 µm pass on conventional Leibovitz's L-15.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final pore size
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-LL15-QPR2X Leibovitz's L-15 2X medium Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15-QPR2X
Applications

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15-QPR2X — Leibovitz's L-15 2X — delivers 0.04 µm filtered purity for primary cell and CO₂-independent applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems — a distinct tier from the Microfluidics Suitable (0.04 µm) product on this page.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates the 0.04 µm tier does not
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Live-Cell Imaging

Open-Stage & CO₂-Free Microscopy

L-15 plus galactose maintains pH without CO₂ — a standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning-disk imaging on open-stage microscopes. Excluding phenol red reduces interference in fluorescence and absorbance channels.

ConfocalTIRFLight-sheetOpen-stage
Cell Biology

Primary Cell Dissociation & Transport

CO₂-free L-15 is a standard dissociation and transport medium for primary cells, tissue pieces, and organoids outside the incubator.

Primary cellsOrganoidsTissue dissociation
Microfluidics

OoC Loading & Priming

CO₂-independent L-15 supports chip loading, priming, and cell seeding outside incubators without pH drift during chip assembly.

OoC loadingChip primingCell seeding
In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is a standard superfusion medium for intravital microscopy, supporting pH stability in open tissue preparations without CO₂ during surgical and imaging procedures.

IntravitalSuperfusionIn vivo
Metabolomics

CO₂-Free Metabolic Studies

A galactose-based energy source supports metabolic studies without CO₂ interference; galactose is commonly used to favor oxidative phosphorylation in metabolically flexible cells.

OXPHOSGalactose forcingMetabolomics
Neuroscience

Acute Brain Slice & Neuron Imaging

CO₂-free L-15 supports neuronal viability in acute brain slice preparations and dissociated neuron imaging without requiring carbogen (95% O₂/5% CO₂) gassing.

Brain slicesPrimary neuronsNeuronal imaging
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Calcium, [+] Magnesium | [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate
Appearance Pale yellow, clear solution
Carbon source D-Galactose — 1800 mg/L (1.8 g/L)
pH USP <791> 7.4
Osmolality USP <785> 620–680 mOsm/kg H₂O
Available pack sizes 500 mL, 1000 mL
Total ingredients 32 components across 4 categories
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent by design — D-galactose and high amino-acid buffering maintain pH without gas supplementation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Leibovitz's L-15 2X: 32 ingredients per lot, with CAS numbers for raw-material traceability. Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino-acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 370.000
Magnesium chloride hexahydrate 7791-18-6 400.000
Magnesium sulfate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Potassium phosphate monobasic 7778-77-0 120.000
Sodium chloride 7647-14-5 16000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 380.240
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 450.000
Glycine 56-40-6 400.000
L-Arginine (free base) 74-79-3 1000.000
L-Asparagine 70-47-3 500.000
L-Cysteine (free base) 52-90-4 240.000
L-Histidine (free base) 71-00-1 500.000
L-Isoleucine 73-32-5 500.000
L-Leucine 61-90-5 250.000
L-Lysine hydrochloride 657-27-2 188.000
L-Methionine 63-68-3 150.000
L-Phenylalanine 63-91-2 250.000
L-Serine 56-45-1 400.000
L-Threonine 72-19-5 600.000
L-Tryptophan 73-22-3 40.000
L-Tyrosine disodium salt 69847-45-6 552.320
L-Valine 72-18-4 200.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.200
Thiamine hydrochloride 67-03-8 2.000
i-Inositol 87-89-8 4.000
OTHERS
D-Galactose 59-23-4 1800.000
Custom formulation: Contact support@diagnocine.com for DCP-LL15-QPR2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity with controlled trace-metal and TOC levels.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 620–680 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-LL15-QPR2X compares

FluxMPS™ DCP-LL15-QPR2X vs. conventional 0.22 µm–filtered Leibovitz's and standard media.

Parameter DCP-LL15-QPR2X (FluxMPS™) Conventional Leibovitz's L-15 (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
Minimal 2X L-15 base — galactose-only carbon source, CO₂-free, researcher-defined nitrogen supplementation check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
CO₂-free design check_circle Yes (galactose) check_circle Yes cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15-QPR2X — Leibovitz's L-15 2X.

Yes. DCP-LL15-QPR2X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size. Its CO₂-free design (D-galactose and high amino-acid buffering) makes it well suited to chip loading, priming, and open-atmosphere OoC platforms where CO₂ control is impractical.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — reaching a 0.04 µm final cut-off, well below conventional 0.22 µm filtration and within the mycoplasma-retentive range (0.1 µm stages, not tested per lot).
This is a minimal 2X L-15 base: D-galactose is the only carbon source, phenol red is absent to reduce optical interference in fluorescence and absorbance channels, and glutamine and pyruvate are left out so you can add them fresh at the concentration your cell type requires. Filter any added stock solution before use per your cell type's protocol.
No. This formulation is CO₂-independent by design — D-galactose and high amino-acid buffering maintain pH under atmospheric conditions without gas supplementation.
Yes. FBS (typically 5–10%), serum-free supplements, growth factors, and other additions can be made after dilution to working strength. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter — never a 0.04 µm membrane, which will strip serum proteins and lipoproteins and clog rapidly. Contact support@diagnocine.com for custom co-formulation.
Each manufacturing batch is released against a specification of < 0.05 EU/mL by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL). This is a batch-level release specification rather than a per-unit result; a Certificate of Analysis for the specific lot is available on request.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X in CO₂-free live-cell imaging, primary cell culture, and OoC applications.

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120325
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
  9. Rasband WS, et al. Live-cell imaging in CO₂-independent media for extended microscopy sessions. Methods Cell Biol. 2015;125:1–18. doi:10.1016/bs.mcb.2014.10.023

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