Inorganic Pyrophosphatase (Yeast)
Cat. No.: SB-G3460-100U
Size: 100U
Description
This product, Inorganic Pyrophosphatase, is inorganic pyrophosphatase (PPase), which is derived from Saccharomyces cerevisiae. It is an enzyme that catalyzes the conversion of one molecule of pyrophosphate into two molecules of phosphate ions (P2 O7 -4 +H2O + PPase → 2HPO4 -2 ) . The reaction catalyzed by PPase is a highly exothermic reaction. Under natural conditions, PPase can provide thermodynamic power for the biosynthesis of proteins, RNA, and DNA, and play a role in biotransformation processes such as lipid metabolism and calcium absorption. The main purpose of this product: to increase the yield of RNA transcribed in vitro, to increase the yield of DNA in PCR reaction, to remove PPi pollutants in reagents for SNP genotyping by pyrophosphate assay, etc.
- Source: Derived from Saccharomyces cerevisiae, recombinantly expressed in Escherichia coli.
- Definition of enzyme activity: Under standard reaction conditions, the amount of enzyme required to hydrolyze PPi to produce 1 μmol Pi per minute is defined as one enzyme activity unit. (Standard reaction conditions: 500 μL system contains 100 mM Tris-HCl, 2 mM MgCl2 , 2 mM PPi, pH 8.0; react at 25°C for 10 min)
- Purity and concentration: SDS-PAGE detection purity > 95%; endogenous nucleic acid residue < 1 pg/μL (qPCR detection); 1 U/μL.
- Enzyme storage buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0.
Storage and Handling Conditions
Transport with wet ice; Store at -20℃, valid for 12 months.
Composition
| Component Number |
Component |
G3460-100U |
| G3460-1 |
Inorganic Pyrophosphatase ( Yeast ) |
100 μL |
| Product Manual |
1 copy |
Steps
1. This product is active in a variety of reaction systems. Usually, in experiments such as in vitro RNA transcription, HAD amplification, and LAMP amplification, an appropriate amount of Inorganic Pyrophosphatase ( Yeast ) can be directly added to the reaction system. The optimum reaction temperature is 25°C, the effective reaction temperature is 16-37°C, and the enzyme activity will be lost if the temperature is higher than 65°C.
2. The amount of the enzyme needs to be optimized in different experiments, usually adjusted at a final concentration of 0.05~1 U/mL.
Precautions
- Enzyme products should be placed in an ice box or ice bath when used, and should be stored at -20°C immediately after use. It is recommended to store in separate packages.
- For your safety and health, please wear a lab coat and disposable gloves for operation.
