.jpg "/Content/Upload/Product/Human_ELISA_kits_Diagnocine_1200(22866).jpg")
Human Rapamycin Insensitive Companion of Mtor, RICTOR ELISA Kit
Product#: BTL-E3592Hu
$686.40
Availability:
Ships in 24 hours
Ready to use
Human Rapamycin Insensitive Companion of Mtor, RICTOR ELISA Kit
Cat. No#: BTL-E3592Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.028ng/ml
Detection range: 0.05-15ng/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Rapamycin insensitive companion of mTOR
SKU#.: ? BTL- |
E3592Hu |
Assay type |
Sandwich |
Size |
0.028ng/ml |
Detection range |
0.05-15ng/ml |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development. |
UniProt accession |
192,218 |
GeneID |
253260 |
Synonyms |
AVO3;AVO3 homolog;hAVO3;PIA;Rapamycin-insensitive companion of mTOR;RICTOR |
Gene names |
RICTOR |
Research area |
Cell Biology |
Target protein |
RICTOR |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3592Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 2.155 | 2.241 | 2.191 |
| 2.327 | |||
| 4ng/ml | 1.327 | 1.353 | 1.303 |
| 1.379 | |||
| 2ng/ml | 0.792 | 0.78 | 0.731 |
| 0.769 | |||
| 1ng/ml | 0.486 | 0.47 | 0.421 |
| 0.455 | |||
| 0.5ng/ml | 0.288 | 0.274 | 0.225 |
| 0.26 | |||
| 0ng/ml | 0.04 | 0.05 | 0 |
| 0.059 |
Precision
We measured random samples of E3592Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 3.5 | 0.16 | 4.48 |
| Intra-Assay | 2 | 18 | 4.77 | 0.14 | 3.03 |
| Intra-Assay | 3 | 18 | 1.77 | 0.11 | 6.44 |