FluxMPS™ Ham's F-12 Nutrient Mixture w/o Phenol Red: 1X Liquid
FluxMPS™ Ham's F-12 Nutrient Mixture w/o Phenol Red: 1X Liquid
Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid FluxMPS™ DCP-H12-R1X is a Ham's F-12 Nutrient Mixture engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final pore size
- Phenol red–free formulation removes phenol red's characteristic absorbance, suited to hormone-sensitive assays and fluorescence-based imaging
- Complete nutrient profile: 1801.60 mg/L D-glucose, 146.00 mg/L L-glutamine, 110.10 mg/L sodium pyruvate, and 1176.00 mg/L sodium bicarbonate
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- 46 verified components across inorganic salts, amino acids, vitamins, and other factors, with CAS traceability
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Custom pH, salt, and nutrient adjustments available on request — contact support@diagnocine.com
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] 1801.60 mg/L Glucose, [+] Sodium Pyruvate | [-] Phenol Red
- AppearanceClear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)280 – 320 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Total ingredients46
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma-range organisms, subvisible particulates, and process residues that clog microfluidic channels and interfere with sensor readouts. FluxMPS™ addresses these failure modes with a four-stage filtration train reaching a 0.04 µm final pore size.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 particulate compliance supports safe perfusion across chip geometries.
Total metabolic control
Glucose, L-glutamine, sodium pyruvate and sodium bicarbonate are all retained at defined concentrations for consistent nutrient and buffer control across your protocol.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) with tight trace-metal and total organic carbon (TOC) control, supporting consistent formulation lot to lot.
Low background for imaging
Ultra-low particulate count keeps background scatter low for confocal and biosensor applications. Phenol red is not added, removing that dye's characteristic absorbance for imaging and hormone-sensitive assays.
Rich, stable nutrient profile
46 ingredients verified per lot across inorganic salts, amino acids, vitamins, and other factors; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration passes reach a final 0.04 µm polish, delivering sub-mycoplasma-range purity beyond what a single-pass 0.22 µm filtration provides.
-
1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates and particulates; protects the first 0.04 µm cartridge and downstream chip geometries.
-
2
0.04 µm Final filtration I — Mycoplasma-Retentive Filtration
Retains particulates and organisms in the 0.2–0.3 µm range, including mycoplasma, by membrane pore size; not tested per lot.
-
3
0.1 µm Prefiltration II — Second-Pass Protection
A dedicated second prefilter protects the second 0.04 µm cartridge from fouling.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-H12-R1X
Designed for next-generation cell models
FluxMPS™ DCP-H12-R1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade variant is available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
- Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
Ultra-clean 0.04 µm–filtered media supports stable perfusion in complex multi-organ chip architectures.
CHO & Mammalian Cell Culture
Suited to CHO, cancer cell lines, primary cells, and clonal growth in serum-free or low-serum conditions.
iPSC-Derived Models
Endotoxin release specification < 0.05 EU/mL supports sensitive iPSC-derived culture protocols.
Endothelial & Primary Cells
Particulate-controlled, endotoxin-specified media supports HUVEC monolayer integrity and TEER monitoring.
Metabolic Flux Analysis
Defined nutrient formulation supports ¹³C isotope tracing and NMR metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium.
Microscopy & Optical Sensing
Ultra-low particulate count and phenol red–free formulation reduce background for confocal and biosensor platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] 1801.60 mg/L Glucose, [+] Sodium Pyruvate | [-] Phenol Red |
| Appearance | Clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 280 – 320 mOsm/kg H2O |
| Glucose | 1801.60 mg/L |
| L-Glutamine | 146.00 mg/L |
| Sodium Pyruvate | 110.10 mg/L |
| Phenol Red | Not added / None |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | Bicarbonate-buffered (1176 mg/L, ~14 mM NaHCO3); approximately 3% CO2 calculated to maintain pH 7.4 (Henderson-Hasselbalch, pKa 6.1). Verify CO2 tension for your incubator and cell line. |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
46 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham's F-12 contains zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 44.100 |
| Copper sulfate pentahydrate | 7758-99-8 | 0.0025 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride anhydrous | 7786-30-3 | 57.650 |
| Potassium chloride | 7447-40-7 | 223.600 |
| Sodium bicarbonate | 144-55-8 | 1176.000 |
| Sodium chloride | 7647-14-5 | 7599.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 142.040 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.863 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.910 |
| L-Arginine hydrochloride | 1119-34-2 | 210.700 |
| L-Asparagine monohydrate | 5794-13-8 | 15.010 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 35.120 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 146.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Isoleucine | 73-32-5 | 3.940 |
| L-Leucine | 61-90-5 | 13.100 |
| L-Lysine hydrochloride | 657-27-2 | 36.500 |
| L-Methionine | 63-68-3 | 4.480 |
| L-Phenylalanine | 63-91-2 | 4.960 |
| L-Proline | 147-85-3 | 34.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 11.900 |
| L-Tryptophan | 73-22-3 | 2.040 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 7.810 |
| L-Valine | 72-18-4 | 11.700 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.0073 |
| Choline chloride | 67-48-1 | 13.960 |
| D-Ca-Pantothenate | 137-08-6 | 0.480 |
| Folic acid | 59-30-3 | 1.320 |
| Nicotinamide | 98-92-0 | 0.037 |
| Pyridoxal hydrochloride | 65-22-5 | 0.062 |
| Riboflavin | 83-88-5 | 0.038 |
| Thiamine hydrochloride | 67-03-8 | 0.340 |
| Vitamin B12 | 68-19-9 | 1.360 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 18.000 |
| D-Glucose | 50-99-7 | 1801.600 |
| Hypoxanthine Sodium Salt | 45738-97-4 | 4.770 |
| Linoleic acid | 60-33-3 | 0.084 |
| Lipoic acid | 1077-28-7 | 0.210 |
| Putrescine dihydrochloride | 333-93-7 | 0.161 |
| Sodium pyruvate | 113-24-6 | 110.100 |
| Thymidine | 50-89-5 | 0.730 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity with tight trace-metal and TOC control for consistent lot-to-lot formulation.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 280 – 320 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-H12-R1X compares
FluxMPS™ DCP-H12-R1X vs. conventional 0.22 µm–filtered Ham's F-12 formulations.
| Parameter | DCP-H12-R1X (FluxMPS™) | Conventional Ham's F-12 (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard filtration (no defined grade) | Standard filtration (no defined grade) |
| Formulation trait | Phenol red-free; glucose, glutamine, pyruvate, bicarbonate retained | Contains phenol red (typical) | Contains phenol red (typical) |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes (Method 1) | cancel Not specified | cancel Not specified |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Yes | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-H12-R1X.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
