FluxMPS™ Ham's F-12 Nutrient Mixture, Coon's Modification w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ Ham's F-12 Nutrient Mixture, Coon's Modification w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ DCP-12C-B1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Ham's F-12 Coon's Modification formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

• Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish, reaching a 0.04 µm final cut-off below the mycoplasma size range (0.2–0.3 µm)
• Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85> Bacterial Endotoxins Test)
• Formulation: [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate
• CO₂-independent: no sodium bicarbonate buffering — use HEPES or another organic buffer for pH control outside a CO₂ incubator
• Ultrapure Type 1 water (18.2 MΩ·cm) and ISO Class 5 (Class 100) aseptic fill under an ISO 13485:2016 quality management system
• 49 verified ingredients across inorganic salts, amino acids, vitamins, and other defined components, released per lot with full traceability
• Custom pH, glucose concentration, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-scale particulates, subvisible aggregates, and residual debris that clog microfluidic channels and interfere with sensor and imaging readouts. FluxMPS™ addresses these failure modes with a validated four-stage filtration train reaching a 0.04 µm final cut-off.

Quadruple-stage filtration system

Four filtration stages, run as two dedicated prefilter-plus-final-filter pairs, reach a final 0.04 µm polish under ISO Class 5 aseptic conditions — well below the 0.2–0.3 µm mycoplasma size range and below the 0.22 µm cut-off of conventional filtered media.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would pass.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge and providing full-train redundancy.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter under aseptic fill & finish conditions (ISO Class 5).

Designed for next-generation cell models

FluxMPS™ DCP-12C-B1X supports platforms from single-channel microfluidic chips to CO₂-independent, HEPES-buffered multi-organ culture systems.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor perfusion and robotic liquid handlers.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
• Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

All 49 ingredients verified per lot with CAS numbers for full raw-material traceability. This Ham's F-12 Coon's Modification formulation contains zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins, and is formulated without sodium bicarbonate.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-12C-B1X compares

FluxMPS™ DCP-12C-B1X vs. conventional 0.22 µm–filtered Ham's F-12 Coon's Modification formulations.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-12C-B1X.

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip and microfluidic research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j