FluxMPS™ Ham's F-12 Nutrient Mixture, Coon's Modification w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Ham's F-12 Nutrient Mixture, Coon's Modification w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-12C-B1X is an MPS-grade, ultra-filtered Ham's F-12 Coon's Modification formulation engineered for microphysiological systems, organ-on-a-chip (OoC), and microfluidic tissue models. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate | [-] Sodium Bicarbonate.
- 0.04 µm final nano-filtration — sub-mycoplasma purity for microfluidic channels below 100 µm
- Quadruple-stage sequential filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Endotoxin < 0.05 EU/mL (LAL, USP <85>)
- Formulation: [+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate | [-] Sodium Bicarbonate
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Custom pH, salts, and nutrient adjustments available on request
- Formulation[+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate | [-] Sodium Bicabonate
- AppearancePink-Red colored, clear solution
- pH (USP <791>)7.4
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Total ingredients49
- Storage2–8°C, away from light
- Shelf Life12 months
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma, subvisible particulates, and endotoxin fragments that clog microfluidic channels and corrupt sensor signals. FluxMPS™ eliminates these failure modes with four-stage sub-0.04 µm filtration.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance ensures safe perfusion in every chip geometry.
Total metabolic control
Selective inclusion/exclusion of glutamine, pyruvate, bicarbonate, and HEPES for precise nutrient and buffer definition.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm), USP <85> — eliminates ionic contaminants interfering with TEER and electrophysiology.
Low background for imaging
Ultra-low particulate count; phenol red–free variants eliminate autofluorescence for confocal and biosensor applications.
Rich, stable nutrient profile
49 ingredients verified per lot; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, HEPES, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma purity unavailable from any conventional 0.22 µm filtered media.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates and particulates; protects downstream 0.04 µm membranes and chip geometries.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) and fine particulates — absent from standard 0.22 µm filtration.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
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4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ DCP-12C-B1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered media, with a confirmed mycoplasma barrier at every production stage.
© Diagnocine® — DCP-12C-B1X
Designed for next-generation cell models
FluxMPS™ DCP-12C-B1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered variant available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Eliminates micro-fouling of solenoid valves and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
Micro Physiological System (MPS) & Chip
Ultra-clean 0.04 µm–filtered media prevents microchannel clogging in complex multi-organ chip architectures.
CHO & Mammalian Cell Culture
Optimized for CHO, cancer cells, primary cells, and clonal growth in serum-free or low-serum conditions.
iPSC-Derived Models
Ultra-low endotoxin (< 0.05 EU/mL) and mycoplasma-free certification for sensitive iPSC protocols.
Endothelial & Primary Cells
Particle-free, endotoxin-controlled media for HUVEC monolayer integrity and TEER monitoring.
Metabolic Flux Analysis
Defined formulation provides a clean background for ¹³C isotope tracing and Seahorse XF assays.
Microscopy & Optical Sensing
Ultra-low particulate and (phenol red–free variants) zero autofluorescence for confocal and biosensor platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate | [-] Sodium Bicarbonate |
| Appearance | Pink-Red colored, clear solution |
| pH USP <791> | 7.4 ± 0.2 |
| Total ingredients | 49 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent; use HEPES or organic buffer for pH control |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
49 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham's F-12 contains zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 165.000 |
| Copper sulphate pentahydrate | 7758-99-8 | 0.0025 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 132.040 |
| Ferric sulphate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride hexahydrate | 7791-18-6 | 106.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 50.800 |
| Potassium chloride | 7447-40-7 | 305.000 |
| Potassium dihydrogen phosphate | 7778-77-0 | 68.000 |
| Sodium chloride | 7647-14-5 | 7530.000 |
| Zinc sulphate heptahydrate | 7446-20-0 | 0.144 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 16.000 |
| L-Alanine | 56-41-7 | 18.000 |
| L-Arginine hydrochloride | 1119-34-2 | 422.000 |
| L-Asparagine anhydrous | 70-47-3 | 30.000 |
| L-Aspartic acid | 56-84-8 | 26.000 |
| L-Cysteine hydrochloride | 52-89-1 | 70.260 |
| L-Glutamic acid | 56-86-0 | 30.000 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 7.800 |
| L-Leucine | 61-90-5 | 26.200 |
| L-Lysine hydrochloride | 657-27-2 | 73.000 |
| L-Methionine | 63-68-3 | 9.000 |
| L-Phenylalanine | 63-91-2 | 10.000 |
| L-Proline | 147-85-3 | 70.000 |
| L-Serine | 56-45-1 | 21.000 |
| L-Threonine | 72-19-5 | 23.800 |
| L-Tryptophan | 73-22-3 | 4.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 15.860 |
| L-Valine | 72-18-4 | 23.400 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.0073 |
| Choline chloride | 67-48-1 | 13.960 |
| D-Ca-Pantothenate | 137-08-6 | 0.480 |
| Folic acid | 59-30-3 | 1.320 |
| L-Ascorbic acid | 50-81-7 | 45.000 |
| Nicotinamide | 98-92-0 | 0.040 |
| Pyridoxine hydrochloride | 58-56-0 | 0.060 |
| Riboflavin | 83-88-5 | 0.040 |
| Thiamine hydrochloride | 67-03-8 | 0.340 |
| Vitamin B12 | 68-19-9 | 1.360 |
| i-Inositol | 87-89-8 | 18.020 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Hypoxanthine sodium salt | 45738-97-4 | 4.040 |
| Linoleic acid | 60-33-3 | 0.090 |
| Lipoic acid | 1077-28-7 | 0.206 |
| Phenol red sodium salt | 34487-61-1 | 1.240 |
| Putrescine dihydrochloride | 333-93-7 | 0.300 |
| Sodium pyruvate | 113-24-6 | 220.000 |
| Thymidine | 50-89-5 | 0.700 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, USP <85> resistivity — eliminates ionic contaminants affecting cell signaling and sensor measurements.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay, release limit: < 0.05 EU/mL per lot.
Particulate — USP <788> Method 2
Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-12C-B1X compares
FluxMPS™ DCP-12C-B1X vs. conventional 0.22 µm–filtered Ham's F-12 Coon's Modification formulations.
| Parameter | DCP-12C-B1X (FluxMPS™) | Conventional Ham's F-12 Coon's Mo (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Coon's Modification without Sodium Bicarbonate — CO₂-free enriched hybrid cell base | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes | cancel No | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Frequently asked questions
Common questions about FluxMPS™ DCP-12C-B1X.
Supporting literature
Key peer-reviewed publications supporting MPS-grade, ultra-filtered media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
