FluxMPS™ Ham's F-12 Nutrient Mixture, Coon's Modification with High Glucose: 1X Liquid
FluxMPS™ Ham's F-12 Nutrient Mixture, Coon's Modification with High Glucose: 1X Liquid
FluxMPS™ DCP-12CHG1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Ham's F-12 Coon's Modification High Glucose formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- High glucose (4.5 g/L / ~25 mM) with 4× Coon's Modification amino acid and vitamin density for hybrid selection and high-density clonal growth
- Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm final polish (Sterile, 4 passes)
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Contains sodium bicarbonate (2676 mg/L) and phenol red (1.24 mg/L); CO₂ requirement derived from the bicarbonate concentration — see Specifications
- 50 verified ingredients across inorganic salts, amino acids, vitamins and specialty additives (zinc, putrescine, hypoxanthine, thymidine)
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Microfluidics Suitable — 0.04 µm final cut-off engineered for microfluidic channels, organ-on-a-chip (OoC) and microphysiological systems (MPS)
- Custom pH, glucose concentration, salts, and nutrient adjustments available on request
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage, Sterile)
- CO₂ Requirement~7% (derived from bicarbonate)
- Storage2–8°C, protected from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma-scale organisms, subvisible particulates, and endotoxin fragments that clog microfluidic channels and corrupt sensor signals. FluxMPS™ reduces these failure modes with four-pass sub-0.04 µm filtration.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 particulate compliance supports safe perfusion across chip geometries.
Total metabolic control
This formulation includes glucose, glutamine, pyruvate, and bicarbonate at defined concentrations — see Composition for the full release-tested profile.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) minimizes trace-metal and organic-carbon (TOC) contaminant load carried into the finished medium.
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy and optical biosensor applications; this formulation contains phenol red (see Composition), which contributes background absorbance in some optical assays.
Rich, stable nutrient profile
50 ingredients verified per lot; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration passes reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma-scale purity beyond what conventional 0.22 µm–filtered media can achieve.
-
1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates and particulates; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
Retains fine particulates and mycoplasma-scale organisms (0.2–0.3 µm) not retained by standard 0.22 µm filtration.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.
Performance vs. conventional media
© Diagnocine® — DCP-12CHG1X
Designed for next-generation cell models
FluxMPS™ DCP-12CHG1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Eliminates micro-fouling of solenoid valves and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
Ultra-clean 0.04 µm–filtered media helps prevent microchannel clogging in complex multi-organ chip architectures.
CHO & Mammalian Cell Culture
Optimized for CHO, cancer cells, primary cells, and clonal growth in serum-free or low-serum conditions.
iPSC-Derived Models
Ultra-low endotoxin (< 0.05 EU/mL) and 0.1 µm mycoplasma-retentive filtration support sensitive iPSC protocols.
Endothelial & Primary Cells
Particle-controlled, endotoxin-controlled media for HUVEC monolayer integrity and TEER monitoring.
Metabolic Flux Analysis
Defined, traceable formulation supports ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate baseline for confocal and biosensor platforms; this formulation contains phenol red, which may contribute background absorbance in some optical assays.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate |
| Appearance | Red-colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Glucose | 4500 mg/L (~25 mM) — High Glucose |
| L-Glutamine | 292 mg/L (~2.0 mM) |
| Sodium Pyruvate | 220 mg/L (~2.0 mM) |
| Phenol Red | 1.24 mg/L (sodium salt) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approximately 7% CO₂ (derived from 31.9 mM sodium bicarbonate at pH 7.4 via Henderson-Hasselbalch; validate empirically per incubator/vessel) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
| Pack sizes available | 500 mL, 1000 mL |
Full composition (mg/L)
50 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham's F-12 Coon's Modification contains zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 165.000 |
| Copper sulfate pentahydrate | 7758-99-8 | 0.0025 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 132.040 |
| Ferric sulfate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride hexahydrate | 7791-18-6 | 106.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 50.800 |
| Potassium chloride | 7447-40-7 | 305.000 |
| Potassium dihydrogen phosphate | 7778-77-0 | 68.000 |
| Sodium bicarbonate | 144-55-8 | 2676.000 |
| Sodium chloride | 7647-14-5 | 7530.000 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.144 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 16.000 |
| L-Alanine | 56-41-7 | 18.000 |
| L-Arginine hydrochloride | 1119-34-2 | 422.000 |
| L-Asparagine anhydrous | 70-47-3 | 30.000 |
| L-Aspartic acid | 56-84-8 | 26.000 |
| L-Cysteine hydrochloride | 52-89-1 | 70.260 |
| L-Glutamic acid | 56-86-0 | 30.000 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 7.800 |
| L-Leucine | 61-90-5 | 26.200 |
| L-Lysine hydrochloride | 657-27-2 | 73.000 |
| L-Methionine | 63-68-3 | 9.000 |
| L-Phenylalanine | 63-91-2 | 10.000 |
| L-Proline | 147-85-3 | 70.000 |
| L-Serine | 56-45-1 | 21.000 |
| L-Threonine | 72-19-5 | 23.800 |
| L-Tryptophan | 73-22-3 | 4.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 15.860 |
| L-Valine | 72-18-4 | 23.400 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.0073 |
| Choline chloride | 67-48-1 | 13.960 |
| D-Ca-Pantothenate | 137-08-6 | 0.480 |
| Folic acid | 59-30-3 | 1.320 |
| L-Ascorbic acid | 50-81-7 | 45.000 |
| Nicotinamide | 98-92-0 | 0.040 |
| Pyridoxine hydrochloride | 58-56-0 | 0.060 |
| Riboflavin | 83-88-5 | 0.040 |
| Thiamine hydrochloride | 67-03-8 | 0.340 |
| Vitamin B12 | 68-19-9 | 1.360 |
| i-Inositol | 87-89-8 | 18.020 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4500.000 |
| Hypoxanthine sodium salt | 45738-97-4 | 4.040 |
| Linoleic acid | 60-33-3 | 0.090 |
| Lipoic acid | 1077-28-7 | 0.206 |
| Phenol red sodium salt | 34487-61-1 | 1.240 |
| Putrescine dihydrochloride | 333-93-7 | 0.300 |
| Sodium pyruvate | 113-24-6 | 220.000 |
| Thymidine | 50-89-5 | 0.700 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity — minimizes trace-metal and organic-carbon contaminant load in the finished medium.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Contact support@diagnocine.com for the current release specification.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-12CHG1X compares
FluxMPS™ DCP-12CHG1X vs. conventional 0.22 µm–filtered Ham's F-12 Coon's Modification High Glucose formulations.
| Parameter | DCP-12CHG1X (FluxMPS™) | Conventional Ham's F-12 Coon's Mod (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Coon's Modification High Glucose — maximum nutrient density for hybrid and fusion protocols | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple, Sterile) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle 0.1 µm retentive | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested (Method 1) | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-12CHG1X.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Coon HG. Clonal culture of differentiated rat liver cells. J Cell Biol. 1968;39:29a.
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
