HEPES-buffered saline

Catalog No.: DCP-HBS1X

Size: 500 mL
Sterile: Filtered 0.1-micron membrane TWICE, 0.04-micron membrane ONCE

We also offer a bundle of the FAO Blue (Fatty Acid Oxidation Detection Reagent) and its buffer: HBS, 1X (HEPES-Buffered Saline), 20mM HEPES. To see indivual pages, please click one of the following:

Product	Catalog	Order
FAO Blue + HBS (HEPES-Buffered Saline) 1X	DCP-FDV-0033-HBS
FAO Blue (Fatty Acid Oxidation Detection Reagent)	FNK-FDV-0033

HEPES-buffered saline is a solution commonly used in cell culture and molecular biology applications. It is designed to maintain a stable pH environment, which is crucial for various biological processes. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic buffering agent known for its ability to maintain physiological pH more effectively than bicarbonate buffers, especially in environments where CO₂ levels cannot be controlled.

Features:

• Buffering Capacity: HEPES is effective in maintaining a stable pH in the range of 6.0 to 8.0, making it suitable for cell culture applications where precise pH control is necessary
• Applications: It is widely used in cell culture, tissue culture, and molecular biology for procedures such as trypsinization, where it helps neutralize complex proteins that may inactivate trypsin.
• Stability: HEPES-buffered saline is chemically and enzymatically stable, with low absorbance in visible and ultraviolet light, making it suitable for applications like flow cytometry

Compositions:

Ingredients	mM	mg/L
HEPES (at pH 7.4)	20	4766.024
Sodium chloride (NaCl)	107	6253.08
Potassium chloride (KCl)	6	447.309
Magnesium sulfate (MgSO4)	1.2	144.439
Calcium chloride (CaCl2)	2	221.960

Quality Control

• Apperance: Clear solution.
• pH (at 1X concentration): 7.4
• Sterility: Filtered 0.1 micron twice and 0.04 micron once in a sterile environment. No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
• Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for morphology and quantitatively by estimating the cell counts and comparing it with a control medium.

Storage and Shelf Life

• Storage: 2-8°C, away from bright light.
• Shipping Condition: Room Temperature
• Shelf life: 24 months.
• Use before the expiry date given on the product label.

* All DiagnoCine Precision Sterile media are filtered-sterilized with a 0.1-micron filtration two times and 0.04 micron once. Thus, mycoplasma contamination is prevented.  The smallest size mycoplasma type can be about 0.2 microns.

* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center.  All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA