Glucose-6-Phosphate Dehydrogenase, Thermostable
Glucose-6-Phosphate Dehydrogenase, Thermostable
Cat. No. FNK-GLD-75-01
Stroage -20°C
Size 1ml
Lot No. 1-H001B
Protein conc. 1.5 mg/ml
Volume 1 ml
Form 20 mM Tris-HCl (pH 8.0), 0.4 M NaCl, 1 mM DTT
Storage -20 oC
Activity 22.2 U/ml (at 65 oC)
Notes For research use only. To prevent the enzyme from being precipitated, use in a solution containing higher than 0.3 M NaCl.
EC No. 1.1.1.49
Substrate glucose-6-phosphate
Reaction product 6-phosphogluconate
Reaction temperature ~70℃
Reaction pH 6.5~8.0
Description
EC No.:1.1.1.49 Substrate: glucose-6-phosphate Reaction product: 6-phosphogluconate Reaction temperature : ~70℃ Reaction pH : 6.5~8.0
Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".
Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.
Features of Thermostable Enzyme
- Highly stable and durable for long term use
- High reactivity at high temperature (70 - 100°C)
- Resistance to organic solvent is expected
- Special instruments to inhibit bacterial growth are not required.
- Reaction speficity is much higher compared with chemical reaction
- Speed of reaction can be accelerated
Activity measurement
Reaction mix (50 mM Tris-HCl (pH 7.5 at 65 oC), 2 mM Glucose-6-phosphate, 1 mM NADP+ , 0.3 M NaCl and appropriate amount of the enzyme) was incubated at 65 oC and A340 was monitored. One unit is defined as the amount of enzyme reducing 1 ?mol of NADP + per minute using ?340=6.22 mM-1 cm-1
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