FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth, 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth, 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ DCP-GMEMH-BN1X is a Microfluidics Suitable, ultra-filtered GMEM (Glasgow's MEM) formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it reaches a 0.04 µm final polish for microchannel-safe purity. This is a high-glucose, bicarbonate-free, 25 mM HEPES-buffered formulation enriched with Tryptose Phosphate Broth (TPB), providing CO₂-independent pH stability (HEPES pKa 7.3 at 37°C).

• Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) delivers a 0.04 µm final polish for microfluidic channel safety.
• Bicarbonate-free, 25 mM HEPES-buffered formulation (pKa 7.3 at 37°C) for CO₂-independent pH stability in open-top chip architectures.
• High-glucose GMEM base (4500 mg/L D-Glucose) enriched with Tryptose Phosphate Broth (2950 mg/L) for peptide and growth-factor support of adherent cell lines such as BHK-21.
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch.
• Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis.
• 31 verified components across inorganic salts, amino acids, vitamins, and other formulation categories, with full raw-material traceability.
• Contains phenol red (15 mg/L) as a visual pH indicator dye — not a phenol red–free formulation.
• Custom pH, salt, HEPES, and nutrient adjustments available on request — contact support@diagnocine.com.

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and process residues that clog microfluidic channels and corrupt sensor signals. FluxMPS™ addresses these failure modes with a four-stage filtration train reaching a 0.04 µm final pore size. HEPES buffering removes CO₂ dependency for open-top chip architectures and atmospheric incubation.

Quadruple-stage filtration system

Four sequential filtration stages, run as two dedicated prefilter-plus-final-filter pairs, reach a final 0.04 µm polish — delivering sub-mycoplasma-range purity beyond what conventional 0.22 µm–filtered media can achieve.

1. 1

   0.1 µm Prefiltration I

   Removes large aggregates and particulates; protects the first 0.04 µm cartridge.

2. 2

   0.04 µm Final filtration I

   Retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a standard 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill & finish.

Designed for next-generation cell models

FluxMPS™ DCP-GMEMH-BN1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. Its 25 mM HEPES buffer suits open-top microfluidic devices and atmospheric CO₂ environments.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for automated bioreactor perfusion and robotic liquid handlers — a separate tier from the Microfluidics Suitable product described on this page.

• Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces fouling risk on solenoid valves and inline optical sensors
• Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

31 ingredients verified per lot with CAS numbers for raw-material traceability. GMEM provides an amino acid and vitamin profile enriched relative to basal MEM; Tryptose Phosphate Broth supplies additional peptide nutrients.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-GMEMH-BN1X compares

FluxMPS™ DCP-GMEMH-BN1X vs. conventional 0.22 µm–filtered GMEM (Glasgow's MEM) formulations.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-GMEMH-BN1X.

Supporting literature

Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip and microfluidic research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j