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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-MEM-QB1X is a Microfluidics Suitable, ultra-filtered Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA) formulation engineered for primary cell culture on organ-on-a-chip (OoC), neuronal chip, and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulated without L-glutamine and without sodium bicarbonate, with low glucose (1.0 g/L), Earle’s Salts, NEAA, and sodium pyruvate included.
- MEM with Earle’s Salts — physiological ionic balance optimized for primary fibroblasts, neurons, epithelial, and vascular cells
- Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high glucose-induced ROS and glycation
- Pre-loaded Non-Essential Amino Acids (NEAA) — reduces de novo synthesis burden and ammonia accumulation in low-serum primary culture
- Formulated without L-glutamine and without sodium bicarbonate — supports fresh glutamine supplementation and CO₂-independent, HEPES-buffered culture systems
- Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size
- Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>)
- Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ
- Custom formulation modifications (pH, glucose, NEAA, HEPES) available on request
- BaseMEM + Earle’s Salts + NEAA
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- Formulation[+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate
[-] L-Glutamine, [-] Sodium Bicarbonate - AppearanceOrange-to-red colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)235–275 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered for primary cell culture where standard media fails
MEM with Earle’s Salts is the primary cell biologist’s medium — but conventional 0.22 µm–filtered MEM passes mycoplasma, subvisible particulates, and endotoxin fragments that cause subtle but significant alterations to primary cell gene expression, activation state, and morphology. FluxMPS™ is built to reduce these risk factors while preserving the full MEM + NEAA nutritional profile that primary cells depend on.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in neuronal, epithelial, and fibroblast chip architectures.
Primary cell–optimized formulation
MEM + Earle’s Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm), controlled for trace metals and organic carbon (TOC) — reducing trace contaminant introduction relevant to sensitive primary cell assays.
Endotoxin release specification
< 0.05 EU/mL per-batch release specification — relevant where LPS-driven TLR4 activation can confound primary cell phenotype, fibroblast-to-myofibroblast transition, and neuronal inflammatory responses.
NEAA reduces metabolic burden
Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation from NEAA synthesis pathways.
Customization on demand
pH, glucose, NEAA concentrations, HEPES, and nutrient modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish. For primary cell culture, sub-mycoplasma filtration is especially relevant — mycoplasma contamination in MEM has been associated with altered cytokine profiles, metabolic shifts, and gene expression changes that can persist undetected for months.
-
1
0.1 µm Prefiltration I
Removes large aggregates and contaminants; protects the first 0.04 µm cartridge and downstream chip geometries from fouling.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains particulates in the mycoplasma size range (0.2–0.3 µm) not addressed by standard 0.22 µm filtration.
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3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge; provides redundancy independent of Stage 1.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill under ISO Class 5 conditions.
Performance vs. conventional MEM
© Diagnocine® — DCP-MEM-QB1X
Primary cell models & OoC applications
FluxMPS™ DCP-MEM-QB1X is built for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s salt–balanced conditions better mimic the physiological microenvironment than DMEM — combined with 0.04 µm filtration purity for particle-free chip perfusion.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor perfusion of primary cell cultures where trace particulates can accelerate chip fouling.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates from primary cell perfusion circuits
- Valve & Sensor Protection: Reduces micro-fouling in delicate neuronal and epithelial chip geometries
- Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture
Inquiry Required: Contact support@diagnocine.com for the MPS Grade 0.01 µm variant.
Neuronal Chips & Brain-on-Chip
MEM + Earle’s Salts + NEAA + low glucose is a physiologically relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.
Epithelium-on-Chip
Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and their barrier integrity in transwell and microfluidic TEER-monitored platforms.
Primary Fibroblast & Stromal Models
MEM with NEAA is a classical culture base for primary dermal, lung, and cardiac fibroblasts — NEAA reduces glutamine-driven ammonia accumulation that can promote myofibroblast differentiation artefacts.
Vascular Cell Culture
Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.
Organ-on-a-Chip & MPS
0.04 µm filtered MEM is intended to reduce particulate clogging in sub-100 µm neuronal and epithelial chip microchannels relative to standard 0.22 µm filtered MEM.
Microscopy & Optical Sensing
Quadruple-stage 0.04 µm filtration provides an ultra-low particulate baseline for confocal, TEER sensor, and biosensor applications on primary cell chips. A phenol red–free formulation is available on request for autofluorescence-sensitive imaging assays.
Analytical release specifications
Every lot released against the full specification matrix below. CoA available on request: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate |
| Appearance | Orange-to-red colored, clear solution |
| Base | MEM + Earle’s Salts + NEAA |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 235–275 mOsm/kg H2O |
| Total ingredients | 35 across 4 categories |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification — see § Manufacturing & Compliance) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1/0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent (sodium bicarbonate-free); supplement with HEPES (15–25 mM) for pH buffering in atmospheric incubation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
MEM with Earle’s Salts and NEAA: 35 ingredients verified per lot with CAS numbers where known. The Amino Acids tab includes both essential amino acids and Non-Essential Amino Acids as one combined group per the source composition table.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.900 |
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Asparagine monohydrate | 5794-13-8 | 15.000 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 31.300 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 51.900 |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 2.000 |
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular care for primary cell–grade purity standards.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, trace-metal and TOC controlled — reduces trace contaminant introduction into sensitive primary cell formulations.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; a Certificate of Analysis is issued for every lot.
Endotoxin — USP <85> BET
LAL assay, assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: 235–275 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-MEM-QB1X compares
FluxMPS™ DCP-MEM-QB1X vs. conventional 0.22 µm–filtered MEM and standard high-glucose DMEM for primary cell OoC applications. Endotoxin figures for other suppliers are published release specifications, attributed below.
| Parameter | DCP-MEM-QB1X (FluxMPS™) | Other suppliers (Conventional MEM & Standard DMEM HG, 0.22 µm filtered) |
|---|---|---|
| Grade | Microfluidics Suitable | Standard grade (not filtration-tiered) |
| Formulation without L-glutamine and sodium bicarbonate, with NEAA and low glucose | check_circle Yes | Conventional MEM: NEAA optional add-on Standard DMEM HG: NEAA not included |
| Glucose | 1.0 g/L (Low, physiological) | Conventional MEM: 1.0 g/L Standard DMEM HG: 4.5 g/L (High) |
| Final filtration pore size | 0.04 µm | Conventional MEM: 0.22 µm Standard DMEM HG: 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | Conventional MEM: 1 Standard DMEM HG: 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1/0.04 µm stages) | Conventional MEM: No Standard DMEM HG: No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
| USP <788> particulate tested | check_circle Yes | Conventional MEM: No Standard DMEM HG: No |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Not specified Not specified |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | Conventional MEM: Risk of clogging Standard DMEM HG: Risk of clogging |
| Custom formulation | check_circle Yes | Conventional MEM: No Standard DMEM HG: No |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-MEM-QB1X — MEM with Earle’s Salts and NEAA.
Supporting literature
Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j