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- FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
MPS-grade, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Approximately 5× lower particulate count than conventional 0.22 µm-filtered media.
- Quadruple-stage 0.04 µm final filtration — mycoplasma-free, sub-micron particle–free
- Endotoxin: NMT 5EU/ml (USP <85> BET lot release)
- Ultrapure Type 1 water (18.2 MΩ·cm) — zero ionic background for biosensor chips
- USP <788> Method 2 particulate compliance — microchannel-safe down to 50 µm
- ISO Class 5 (Class 100) laminar-flow aseptic fill & finish
- 4× BME amino acid & vitamin concentrations for robust MPS perfusion
- Custom pH, glucose, HEPES, salts & nutrients — support@diagnocine.com
- GlucosePresent
- L-Glutamine292 mg/L ([+])
- Sodium PyruvateRemoved ([-])
- pH (USP <791>)7.4
- Osmolality (USP <785>)235.00 -275.00 mOsm/kg
- Endotoxin (USP <85>)NMT 5EU/ml
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, away from bright light
- Shelf Life2-8°C, away from bright light
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.1–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.
Microchannel-safe purity
0.04 µm final filter and USP <788> particulate compliance ensure unobstructed flow in channels as narrow as 50 µm.
Total metabolic control
Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing without background noise.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm, USP <85>) — eliminating ionic interference in TEER and electrochemical biosensors.
Low background for imaging
Ultra-low particulate baseline for pristine confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
The only ready-to-use cell culture medium with a validated four-stage sequential filtration reaching a final pore size of 0.04 µm — capturing what 0.22 µm filtration misses entirely.
-
1
0.1 µm Pre-filtration I
Removes large particulates, cell debris, and protein aggregates. Protects downstream fine filters and preserves microchannel chip geometries.
-
2
0.04 µm Pre-filtration II
Retains fine particulates, bacteria, and mycoplasma (typical diameter 0.1–0.3 µm) — entirely absent from standard 0.22 µm media.
-
3
0.1 µm Sterile-filtration I
Second-pass redundant sterile filtration for validated double bioburden clearance.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate polishing filter; aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstation.
Performance vs. conventional media
By USP <788> Method 2, FluxMPS™ delivers ~5× fewer particles ≥10 µm vs standard 0.22 µm filtered media — the difference between successful long-duration perfusion and channel occlusion within 48 h.
© Diagnocine® — DCP-MEMGH-PB1X
Full Composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 36 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
INORGANIC SALTS
| Component | CAS Number | mg/L |
|---|---|---|
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 10049-21-5 | 122.000 |
AMINO ACIDS
| Component | CAS Number | mg/L |
|---|---|---|
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.900 |
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Asparagine monohydrate | 5794-13-8 | 15.000 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 31.300 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 51.900 | |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| i-Inositol | 87-89-8 | 2.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4500.000 |
| HEPES | 7365-45-9 | 5958.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
Full technical specification
Every lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is released against comprehensive multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] Earle's Salts, NEAA, High Glucose, L-Glutamine, 25mM HEPES / [-] Sodium Pyruvate, Sodium Bicarbonate |
| Appearance | Clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 235.00 -275.00 mOsm/kg H₂O |
| Glucose | Present |
| L-Glutamine | 584 mg/L ([+]) |
| Sodium Pyruvate | Removed ([-]) |
| Phenol Red | 15.9 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | NMT 5EU/ml |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma USP <63> | Prevented — dual 0.04 µm |
| Particulate ≥10 µm <788> | ~5× below 0.22 µm media |
| Particulate ≥25 µm <788> | Compliant |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, away from bright light |
| Freeze–thaw | Do not freeze |
| Shelf life | 2-8°C, away from bright light |
| Shipping | Cold pack (2–8°C) |
| CO₂ requirement | 5% CO₂ incubator required |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory | 21 CFR Part 820 aligned; CE-approved |
| Production | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Optimized for next-generation cell biology platforms
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.
Organ-on-a-Chip (OoC) & MPS
Ultra-low particulate, mycoplasma-free media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Glucose-controlled or glucose-free formulations for Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-free perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined formulations for ¹³C metabolic tracing, Seahorse XF respiratory assays, and NMR metabolomics.
Microscopy & Optical Sensing
Optically clear, low-autofluorescence medium for confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Manufacturing & compliance
Every batch subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified, CE-approved suppliers. Final packaging, QA and testing at DiagnoCine R&D Center; customization at DiagnoCine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, <0.001 ppm TOC, USP <85> endotoxin-tested. Eliminates ionic background that corrupts biosensor chips and TEER systems.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves all filtration gains in the final container.
Micro-Batch Precision
Small-batch production: osmolality (±5 mOsm/kg), pH (±0.05), endotoxin tight — critical for reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL kinetic turbidimetric method. Lot release: NMT 5EU/ml. FluxMPS target: <0.05 EU/mL.
Particulate — USP <788> Method 2
~5× lower particles ≥10 µm vs 0.22 µm filtered media by light-obscuration count.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 235.00 -275.00 mOsm/kg H₂O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
How DCP-MEMGH-PB1X compares
Critical advantages in filtration, mycoplasma barrier, water quality, and QC depth that conventional media cannot match for MPS and microfluidic applications.
| Parameter | DCP-MEMGH-PB1X (FluxMPS™) | Standard DMEM (0.22 µm) | Competitor DMEM (0.22 µm) |
|---|---|---|---|
| Formulation definition | [+] Earle's Salts, , NEAA, High Glucose, L-Glutamine, 25mM HEPES / [-] Sodium Pyruvate, Sodium Bicarbonate | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle | cancel | cancel |
| Endotoxin spec | NMT 5EU/ml | Typically <1 EU/mL | Varies |
| USP <788> particulate | check_circle ~5× lower | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel validated | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Frequently asked questions
Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid (DCP-MEMGH-PB1X).
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
