FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Minimum Essential Medium (MEM) formulated with Earle's Salts, high glucose, non-essential amino acids (NEAA) and 25 mM HEPES, without sodium pyruvate or sodium bicarbonate. Engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

• Quadruple-stage filtration reaching a 0.04 µm final pore size (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, four validated passes)
• Endotoxin release specification: < 0.05 EU/mL (USP <85> BET, tested per manufacturing batch)
• High-glucose (4.5 g/L) MEM with Earle's Salts, NEAA and 25 mM HEPES; bicarbonate-free and sodium-pyruvate-free formulation
• HEPES-buffered for reduced CO₂ dependence — suited to ambient-air or custom-CO₂ culture protocols
• Manufactured under an ISO 13485:2016 quality management system; final packaging and QC at Diagnocine, Totowa, NJ
• 4× BME amino acid and vitamin concentrations for robust long-duration MPS perfusion
• Custom pH, glucose, HEPES, salts & nutrients available — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — capturing particulates and bioburden that standard 0.22 µm filtration misses entirely.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge and preserves microchannel chip geometries.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains fine particulates and sub-micron bioburden, including mycoplasma-sized organisms (0.2–0.3 µm), that pass unimpeded through standard 0.22 µm media.

3. 3

   0.1 µm Prefiltration II

   A second dedicated prefilter, protecting the second 0.04 µm final filter cartridge for full process redundancy.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

For fully automated perfusion systems and robotic liquid handling, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, built on a six-stage cascade beyond the standard 0.04 µm Microfluidics Suitable line.

• Total particulate exclusion at the 10 nm scale
• Valve & sensor protection in automated fluidic systems
• Extended perfusion stability for long-duration unattended runs

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to discuss availability.

Full technical specification

Every batch of FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Available sizes: 500 mL, 1000 mL.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 36 components across 4 categories. All values are per-batch verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch is subjected to multi-parameter batch-release testing before distribution.

How DCP-MEMGH-PB1X compares

Differences in filtration, mycoplasma-retentive filtration, water quality, and QC depth relevant to microfluidic and MPS applications.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid (DCP-MEMGH-PB1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x