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FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate, Calcium Chloride: 1X Liquid

Product#: DCP-DMF12H-BCT1X
$52.80
DCP-DMF12H-BCT1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate, Calcium Chloride: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains 15mM HEPES Contains Magnesium Contains 3.8255 g/L Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without Calcium Chloride

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). HEPES-buffered (15 mM) and formulated without sodium bicarbonate or calcium chloride for reduced CO2 dependence.

  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) — 0.04 µm final pore size for microchannel-safe purity
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • 3.8255 g/L glucose (DMEM/F-12, 3:1 base), 474.5 mg/L L-glutamine, 27.525 mg/L sodium pyruvate
  • 15 mM HEPES buffering; formulated without sodium bicarbonate and without calcium chloride
  • Phenol red sodium salt included (12.235 mg/L) as a pH indicator dye
  • Manufactured under an ISO 13485:2016 quality management system; final QC and customization at Diagnocine, Totowa, NJ
  • Custom pH, glucose, HEPES, salts & nutrients available — support@diagnocine.com
DCP-DMF12H-BCT1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate, Calcium Chloride: 1X Liquid
  • Glucose3825.500 mg/L (3.8255 g/L) ([+])
  • L-Glutamine474.500 mg/L ([+])
  • Sodium Pyruvate27.525 mg/L ([+])
  • HEPES15 mM (3574.500 mg/L) ([+])
  • Osmolality (USP <785>)260 - 300 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2 (Quadruple-stage)
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2–8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (typically 0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

filter_alt

Microchannel-safe purity

0.04 µm final filter and USP <788> Method 1 (light obscuration) particulate testing support unobstructed flow in narrow microchannels.

target

Total metabolic control

Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) with rigorous trace-metal and total organic carbon (TOC) control for demanding analytical applications.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.

science

Rich, stable nutrient profile

4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train — two paired 0.1 µm prefilter / 0.04 µm final-filter cycles run in series — reaching a final pore size of 0.04 µm.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow environment.

Performance vs. conventional media

The paired 0.1 µm / 0.04 µm filtration train runs twice in series, reaching a validated 0.04 µm final pore size — well below the 0.22 µm cut-off used in conventional cell culture media, and within range of mycoplasma-scale organisms (0.2–0.3 µm).

4
Sequential filtration stages (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final filter — sub-mycoplasma polishing
Sterility & Mycoplasma: 14-day USP <71> sterility tested per lot. The 0.1 µm stages provide mycoplasma-retentive filtration (not tested per lot); mycoplasma diameter is typically 0.2–0.3 µm.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DMEM/F-12 (3:1) HEPES-buffered, bicarbonate-free, calcium-chloride-free 1X liquid cell culture medium (DCP-DMF12H-BCT1X) - Quadruple-stage filtration system (0.1 micron x2 + 0.04 micron x2) for organ-on-a-chip and microfluidic MPS cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → ISO Class 5 aseptic fill.
© Diagnocine® — DCP-DMF12H-BCT1X
Formulation

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 50 components across 4 category groups (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 tabs below. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

INORGANIC SALTS
Component CAS Number mg/L
Copper sulphate pentahydrate 7758-99-8 0.000625
Disodium hydrogen phosphate anhydrous 7558-79-4 35.510
Ferric nitrate nonahydrate 7782-61-8 0.075
Ferrous sulphate heptahydrate 7782-63-0 0.209
Magnesium chloride hexahydrate 7791-18-6 14.412
Magnesium sulphate anhydrous 7487-88-9 73.290
Potassium chloride 7447-40-7 356.000
Sodium chloride 7647-14-5 6699.750
Sodium dihydrogen phosphate monohydrate 10049-21-5 81.750
Zinc sulphate heptahydrate 7446-20-0 0.215
AMINO ACIDS
Component CAS Number mg/L
Glycine 56-40-6 24.380
L-Alanine 56-41-7 2.227
L-Arginine hydrochloride 1119-34-2 115.520
L-Asparagine monohydrate 5794-13-8 3.752
L-Aspartic acid 56-84-8 3.325
L-Cysteine dihydrochloride 7048-04-6 8.780
L-Cystine hydrochloride monohydrate   46.927
L-Glutamic acid 56-86-0 3.675
L-Glutamine 56-85-9 474.500
L-Histidine hydrochloride monohydrate 5934-29-2 36.740
L-Isoleucine 73-32-5 79.735
L-Leucine 61-90-5 82.025
L-Lysine hydrochloride 657-27-2 118.625
L-Methionine 63-68-3 23.625
L-Phenylalanine 63-91-2 50.740
L-Proline 147-85-3 8.625
L-Serine 56-45-1 34.125
L-Threonine 72-19-5 74.225
L-Tryptophan 73-22-3 12.510
L-Tyrosine disodium salt   79.795
L-Valine 72-18-4 73.425
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 6.490
D-Biotin 58-85-5 0.001825
D-Ca-Pantothenate 137-08-6 3.120
Folic acid 59-30-3 3.330
Nicotinamide 98-92-0 3.000
Pyridoxine hydrochloride 58-56-0 3.015
Riboflavin 83-88-5 0.309
Thiamine hydrochloride 67-03-8 3.085
Vitamin B12 68-19-9 0.340
myo-Inositol 87-89-8 9.900
OTHERS
D-Glucose 50-99-7 3825.500
DL-Thioctic acid 1077-28-7 0.053
HEPES buffer 7365-45-9 3574.500
Hypoxanthine 68-94-0 1.200
Linoleic acid 60-33-3 0.021
Phenol red sodium salt 34487-61-1 12.235
Putrescine hydrochloride 333-93-7 0.040
Sodium pyruvate 113-24-6 27.525
Thymidine 50-89-5 0.183
Custom formulations: pH, glucose, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Technical Specifications

Full technical specification

Every lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate, Calcium Chloride: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, Phenol Red, 15mM HEPES, Magnesium, 3.8255 g/L Glucose, Sodium Pyruvate / [-] Sodium Bicarbonate, Calcium Chloride
Appearance Clear solution with characteristic phenol red coloration (pH indicator dye)
Osmolality USP <785> 260 - 300 mOsm/kg H₂O
Glucose 3825.500 mg/L (3.8255 g/L) ([+])
L-Glutamine 474.500 mg/L ([+])
Sodium Pyruvate 27.525 mg/L ([+])
Phenol Red 12.235 mg/L ([+])
HEPES 15 mM (3574.500 mg/L) ([+])
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> < 0.05 EU/mL (batch release specification — see Manufacturing & Compliance)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm <788> M1 Compliant (USP <788> Method 1, light obscuration)
Particulate ≥25 µm <788> M1 Compliant (USP <788> Method 1)
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, protect from light
Freeze–thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2–8°C)
CO2 requirement HEPES-buffered; reduced CO2 dependence (validate per cell line)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Traceability Full lot traceability; CoA available per batch
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch; Totowa, NJ, USA
Intended use RUO only

Available pack sizes: 500 mL, 1000 mL

Applications

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate, Calcium Chloride: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated perfusion systems and robotic liquid handling, an optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available — see the Grade note in the Purity Architecture section above.

  • Total particulate exclusion for long-duration closed-loop perfusion
  • Valve & sensor protection in automated fluidic handling systems
  • Extended perfusion stability for multi-day, unattended culture runs

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to discuss requirements.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low particulate media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.

OoCToCBoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined glucose and pyruvate levels support Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-free perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium.

¹³C tracingNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate medium for confocal microscopy, fluorescent biosensors, and automated imaging on chip.

ConfocalBiosensorsTEER
Quality Assurance

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity with low total organic carbon (TOC) control, supporting trace-metal-sensitive assays and biosensor-based readouts.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains through final fill.

assignment

Micro-Batch Precision

Small-batch production supports tight lot-to-lot consistency in osmolality, endotoxin, and nutrient concentration — critical for reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL assay. Assay sensitivity: 0.005 EU/mL. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count method. Compliant with USP <788> Method 1 limits.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 260 - 300 mOsm/kg H₂O.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
CoA Request: Available at no charge for any production lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate, Calcium Chloride: 1X Liquid (DCP-DMF12H-BCT1X). Email support@diagnocine.com.
Product Comparison

How DCP-DMF12H-BCT1X compares

Critical advantages in filtration, mycoplasma-risk mitigation, water quality, and QC depth relative to conventional media for MPS and microfluidic applications.

Parameter DCP-DMF12H-BCT1X (FluxMPS™) Standard DMEM/F-12 (0.22 µm) Competitor DMEM/F-12 (0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
Formulation [+] L-Glutamine, Phenol Red, 15mM HEPES, Magnesium, 3.8255 g/L Glucose, Sodium Pyruvate / [-] Sodium Bicarbonate, Calcium Chloride Standard Standard
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Method 1 compliant cancel cancel
Water quality Ultrapure Type 1, 18.2 MΩ·cm Unspecified Unspecified
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle On request cancel cancel

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Sodium Bicarbonate, Calcium Chloride: 1X Liquid (DCP-DMF12H-BCT1X).

DCP-DMF12H-BCT1X is engineered for organ-on-a-chip (OoC), microfluidic, and microphysiological system (MPS) applications. Its quadruple-stage filtration reaches a 0.04 µm final pore size, reducing sub-micron particulates and mycoplasma-scale organisms (0.2–0.3 µm) that can accumulate in narrow microchannels typical of chip-based culture systems.
Four sequential filtration stages (0.1 µm ×2 + 0.04 µm ×2) target particulates and mycoplasma-scale organisms (0.2–0.3 µm) that pass through conventional 0.22 µm filters. Particulate content is evaluated under USP <788> Method 1 (light obscuration).
This formulation replaces sodium bicarbonate buffering with 15 mM HEPES (already included) and omits calcium chloride, supporting applications that benefit from reduced CO2 dependence or calcium-sensitive assay conditions such as cell dissociation. If your application requires sodium bicarbonate buffering or added calcium, contact support@diagnocine.com to discuss a custom formulation.
No standard CO2 requirement applies: this formulation is buffered with 15 mM HEPES rather than sodium bicarbonate, allowing reduced dependence on CO2 for pH stabilization. Validate CO2 conditions for your specific cell line. Custom sodium bicarbonate-buffered formulations are available on request.
Yes. DCP-DMF12H-BCT1X is a basal medium compatible with FBS, human serum, GlutaMAX, growth factors, and antibiotics. Filter serum and protein-containing supplements through a 0.2 µm low-protein-binding PES or PVDF filter before combining; defined, protein-free additions may be filtered at 0.1 µm. Do not use 0.04 µm filters for supplements — this pore size retains serum proteins, lipoproteins, and immunoglobulins.
Every batch is tested by LAL assay (USP <85>, Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before distribution. Endotoxin is controlled per manufacturing batch, and lot-specific results are reported on the Certificate of Analysis available at support@diagnocine.com.
Yes — a full Certificate of Analysis is available per lot, including lot number, expiry, appearance, osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), mycoplasma-retentive filtration status, and particulate count (USP <788> Method 1). Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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