FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o Sodium Pyruvate: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o Sodium Pyruvate: 1X Liquid
FluxMPS™ DCP-DMEM-P1X is a Microfluidics Suitable, quadruple-stage ultra-filtered DMEM High Glucose (4.5 g/L) formulation engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and energy-demanding cell models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] Sodium Pyruvate.
- High Glucose (4.5 g/L) — supports energy-demanding cell types: primary neurons, cardiomyocytes, cancer lines
- 0.04 µm final filtration — pore size well below the 0.2–0.3 µm mycoplasma size range, supporting microfluidic channel safety below 100 µm
- Four-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II (Polish)
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] Sodium Pyruvate
- Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and TOC content; ISO 13485:2016 QMS; ISO Class 5 (Class 100) aseptic fill
- Custom pH, salts, glucose concentration, and nutrient adjustments available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] High Glucose | [-] Sodium Pyruvate
- AppearanceRed-colored (phenol red indicator), clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protected from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma-scale contaminants, subvisible particulates, and endotoxin fragments that can accumulate in microfluidic channels and disrupt sensor signals. FluxMPS™ High Glucose is engineered to reduce these risks while delivering 4.5 g/L glucose for energy-demanding cell types and complex tissue models.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 particulate compliance supports safe perfusion in chip geometries, including sub-100 µm channels.
High-energy cell support
4.5 g/L glucose (approximately 4× standard low-glucose DMEM) sustains primary neurons, cardiomyocytes, cancer lines, and other aerobically active cells in prolonged perfusion.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon (TOC) content, minimizing extraneous inputs into sensitive cell-based assays.
Low background for imaging
Ultra-low particulate baseline (0.04 µm final filtration) supports confocal microscopy and biosensor platforms. This formulation contains phenol red; request our phenol-red-free variant for autofluorescence-sensitive imaging assays.
Rich, stable nutrient profile
32 ingredients verified per lot; 4× BME amino acid/vitamin concentration; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, HEPES, and nutrients adjustable. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four sequential filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm pore size under aseptic fill conditions.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris and protein aggregates; protects the first 0.04 µm final-filter cartridge from early fouling.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates — including material near the 0.2–0.3 µm mycoplasma size range — that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge from fouling.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill under ISO Class 5 (Class 100) conditions.
Performance vs. conventional media
© Diagnocine® — DCP-DMEM-P1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEM-P1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. High glucose (4.5 g/L) makes it particularly suited to energy-intensive cell types and aerobically active cultures.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers — a separate tier from the Microfluidics Suitable (0.04 µm) product on this page.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and optical sensors
- Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Organ-on-a-Chip & MPS
0.04 µm-filtered media helps reduce microchannel clogging risk in complex multi-organ chip architectures.
Warburg Effect & Cancer Models
High glucose (4.5 g/L) supports aerobic glycolysis phenotyping in cancer cell lines requiring elevated energy substrates.
Primary Neurons & Brain-on-Chip
High glucose supports the metabolic demands of primary neurons and iPSC-derived neuronal networks in microfluidic perfusion.
Cardiomyocyte & Heart-on-Chip
High-glucose DMEM is a standard basal medium for iPSC-derived cardiomyocyte maturation and heart-on-chip functional assays.
Metabolic Flux Analysis
Defined high-glucose formulation without sodium pyruvate supports ¹³C isotope tracing and NMR metabolomics profiling. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate baseline supports confocal microscopy and biosensor platforms. Contains phenol red; request our phenol-red-free variant for autofluorescence-sensitive assays.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) | [-] Sodium Pyruvate |
| Appearance | Red-colored (phenol red indicator), clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H2O |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| L-Glutamine | 584 mg/L (~4 mM) |
| Sodium Pyruvate | Not added (excluded; supplement as needed) |
| Phenol Red | 15.9 mg/L (pH indicator) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (release specification, per batch) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approximately 10% CO2 (calculated from 3700 mg/L / 44 mM sodium bicarbonate via Henderson-Hasselbalch to maintain pH 7.4); verify empirically for your system |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations, plus glycine, serine, and ferric nitrate. 32 ingredients verified per lot with CAS numbers for full raw-material traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA and testing at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity with controlled trace-metal and TOC content, supporting reproducible cell culture performance.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per manufacturing batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 310–350 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-DMEM-P1X compares
FluxMPS™ DCP-DMEM-P1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEM-P1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard Alt. DMEM HG (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| High Glucose DMEM without Sodium Pyruvate — researcher-defined secondary carbon source | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle 0.1 µm / 0.04 µm train | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Higher clogging risk | cancel Higher clogging risk |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEM-P1X DMEM High Glucose.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
