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- FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/ L-Alanyl-Glutamine: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/ L-Alanyl-Glutamine: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/ L-Alanyl-Glutamine: 1X Liquid
FluxMPS™ DCP-DMEMYA1X is a Microfluidics Suitable, ultra-filtered DMEM High Glucose — L-Alanyl-Glutamine formulation engineered for long-duration organ-on-a-chip and bioreactor perfusion, where a stable, ammonia-free nitrogen source is essential. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] High Glucose (4500 mg/L), [+] L-Alanyl-Glutamine (dipeptide), [+] Sodium Pyruvate, [+] Sodium Bicarbonate.
- L-Alanyl-L-Glutamine (dipeptide, CAS 39537-23-0) — stable at 37°C, does not degrade to pyrrolidone-5-carboxylic acid and ammonia the way free L-glutamine does
- No ammonia accumulation — avoids ammonia-driven growth inhibition and glycosylation artefacts during long-duration perfusion
- Cleaved to L-glutamine + L-alanine by cell-surface dipeptidases — bioequivalent nitrogen source to free L-glutamine
- Suited to organ-on-a-chip, bioreactor perfusion, and any culture lasting more than 7 days where glutamine stability matters
- Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- Manufactured under an ISO 13485:2016 quality management system with ISO Class 5 (Class 100) aseptic fill
- Custom formulation modifications available on request — contact support@diagnocine.com
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- Key featureL-Alanyl-Glutamine (dipeptide)
- Formulation[+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Stable glutamine — no degradation, no ammonia accumulation
Standard DMEM contains free L-glutamine, which spontaneously degrades in aqueous solution to pyrrolidone-5-carboxylic acid and ammonia at 37°C (half-life approximately 3 weeks at 4°C; days at 37°C). Ammonia accumulation inhibits cell growth and alters glycosylation, confounding metabolic assays. L-Alanyl-L-Glutamine (dipeptide, CAS 39537-23-0) is a stable dipeptide that does not degrade this way — it is cleaved to L-glutamine + L-alanine by dipeptidases at the cell surface, providing a sustained, stable nitrogen source. FluxMPS™ pairs this stable-glutamine formulation with 0.04 µm final filtration for long-duration OoC perfusion where glutamine stability is critical.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in chip geometries, including sub-100 µm channels.
Precise formulation control
L-Alanyl-Glutamine in place of free L-glutamine — a defined substitution enabling research applications that require a stable, non-degrading nitrogen source.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic-carbon (TOC) content, reducing background contributed by the water itself.
Low endotoxin release specification
< 0.05 EU/mL endotoxin release specification, tested by LAL assay (USP <85>) per manufacturing batch.
High-glucose energy support
4.5 g/L glucose sustains the energy demands of glycolytically active cell types and extended perfusion protocols.
Customization on demand
pH, glucose, salts, and additional component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages, run as two prefilter-plus-final-filter pairs, reaching a final 0.04 µm polish under aseptic conditions — delivering ultra-low particulate levels beyond what conventional 0.22 µm filtered media can achieve.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris and protein clumps; protects the first 0.04 µm final-filter cartridge from fouling.
-
2
0.04 µm Final filtration I
First 0.04 µm final-filtration pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter. Together with the upstream 0.1 µm stage, this provides mycoplasma-retentive filtration (organisms in the 0.2–0.3 µm range); not tested per lot.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge and providing redundant large-particulate removal.
-
4
0.04 µm Final filtration II — Polish
Ultimate 0.04 µm polishing filter; aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-DMEMYA1X
Specialized applications requiring stable-glutamine DMEM
FluxMPS™ DCP-DMEMYA1X is designed for research protocols where L-Alanyl-Glutamine stability is essential — combined with Microfluidics Suitable filtration purity for organ-on-a-chip and microfluidic platform compatibility.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor perfusion and robotic liquid handlers — the separate ultra nano-filtered tier described in the grade note above.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
- Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion circuits
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Organ-on-a-Chip & MPS
0.04 µm filtration reduces microchannel clogging risk. L-Alanyl-Glutamine formulation delivered with low particulate content for specialized OoC protocols.
Organ-on-a-Chip Long-Duration Perfusion
FluxMPS™ DCP-DMEMYA1X delivers stable L-Alanyl-Glutamine nutrition with Microfluidics Suitable filtration purity for demanding long-duration perfusion platforms.
Bioreactor Continuous Culture
Ammonia-free nitrogen delivery over extended culture periods supports continuous bioreactor operation without periodic glutamine re-dosing.
iPSC Differentiation
Stable nitrogen source and low-particulate filtration support extended differentiation protocols sensitive to metabolic byproduct accumulation.
Glycoprotein Production
Avoiding ammonia accumulation helps preserve consistent N-glycosylation patterns in glycoprotein-producing cell lines over long culture runs.
CHO Long-Term Culture
Stable-glutamine formulation reduces ammonia-driven growth inhibition in CHO cultures maintained over multi-day to multi-week production runs.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate |
| Appearance | Red-colored, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| Key feature | L-Alanyl-Glutamine (dipeptide, 862 mg/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| L-Glutamine (free) | Not added — replaced by L-Alanyl-Glutamine dipeptide |
| Sodium Pyruvate | 110 mg/L |
| Phenol Red | 15.900 mg/L (phenol red sodium salt) |
| Total ingredients | 33 (7 Inorganic Salts, 15 Amino Acids, 8 Vitamins, 3 Others) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification; see Manufacturing & Compliance) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Controlled by 0.1 µm + 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approximately 10% CO2 (calculated from 3700 mg/L sodium bicarbonate to maintain pH 7.4; validate per incubator and cell line) |
| Pack sizes | 500 mL, 1000 mL |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose — L-Alanyl-Glutamine: 33 ingredients verified per lot with CAS numbers for raw-material traceability. L-Alanyl-L-Glutamine (dipeptide, CAS 39537-23-0) replaces free L-glutamine at an equivalent molar nitrogen contribution. Do not add additional free L-glutamine unless specifically required.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Alanyl-Glutamine | 39537-23-0 | 862.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016–certified quality system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm with controlled trace-metal and TOC content.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — Certificate of Analysis available for every lot.
Endotoxin — USP <85> BET
LAL assay per manufacturing batch; release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMYA1X compares
FluxMPS™ DCP-DMEMYA1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMYA1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm final cut-off) | Standard grade (no filtration tier designation) | Standard grade (no filtration tier designation) |
| Stable-glutamine formulation (L-Alanyl-Glutamine, ammonia-free) | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1/0.04 µm) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle Method 1 tested | cancel Not specified | cancel Not specified |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Higher clogging risk | cancel Higher clogging risk |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMYA1X — DMEM High Glucose with L-Alanyl-Glutamine.
Supporting literature
Key publications supporting the research applications of FluxMPS™ DCP-DMEMYA1X.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ozturk SS, Riley MR, Palsson BO. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production. Biotechnol Bioeng. 1992;39:418–431. doi:10.1002/bit.260390408
- Christie A, Butler M. Glutamine-based dipeptides are utilized by mammalian cells by extracellular hydrolysis catalyzed by a specific peptidase. J Biotechnol. 1994;37:277–290. doi:10.1016/0168-1656(94)90032-9
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Andersen DC, Goochee CF. The effect of ammonia on the O-linked glycosylation of granulocyte colony-stimulating factor produced by Chinese hamster ovary cells. Biotechnol Bioeng. 1995;47:96–105. doi:10.1002/bit.260470112
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
