- Home
- FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/ L-Alanyl-Glutamine: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/ L-Alanyl-Glutamine: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/ L-Alanyl-Glutamine: 1X Liquid
FluxMPS™ DCP-DMEMYA1X is an MPS-grade, ultra-filtered DMEM High Glucose — L-Alanyl-Glutamine formulation engineered for specialized research applications where precise formulation control is essential. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] High Glucose (4500 mg/L), [+] L-Alanyl-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate.
- L-Alanyl-L-Glutamine (dipeptide) — stable at 37°C, no pyrrolidone-5-carboxylic acid degradation
- No ammonia accumulation — prevents growth inhibition and glycosylation artefacts in long-duration perfusion
- Cleaved to L-glutamine + L-alanine by cell-surface dipeptidases — bioequivalent nitrogen source to free L-glutamine
- Ideal for organ-on-a-chip, bioreactor perfusion, and any culture lasting >7 days where glutamine stability matters
- 0.04 µm final nano-filtration — sub-mycoplasma purity for microfluidic channels below 100 µm
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Endotoxin < 0.05 EU/mL (LAL, USP <85>)
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Custom formulation modifications available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- Key featureL-Alanyl-Glutamine
- Formulation[+] High Glucose, [+] L-Alanyl-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months
Stable glutamine — no degradation, no ammonia accumulation
Standard DMEM contains free L-glutamine, which spontaneously degrades in aqueous solution to pyrrolidone-5-carboxylic acid and ammonia at 37°C (half-life ~3 weeks at 4°C; days at 37°C). Ammonia accumulation inhibits cell growth, alters glycosylation, and confounds metabolic assays. L-Alanyl-L-Glutamine (dipeptide, CAS 39537-23-0) is a stable dipeptide that does not degrade — it is cleaved to L-glutamine + L-alanine by dipeptidases at the cell surface, providing a sustained, stable nitrogen source. FluxMPS™ delivers this stable-glutamine formulation with 0.04 µm filtration for long-duration OoC perfusion where glutamine stability is critical.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance ensures safe perfusion in every chip geometry, including sub-100 µm channels.
Precise formulation control
L-Alanyl-Glutamine — defined omission enabling specific research applications that require exact control of this medium component.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm), USP <85> — eliminates ionic contaminants that interfere with specialized assays.
Below TLR4 endotoxin threshold
< 0.05 EU/mL endotoxin — prevents LPS-driven inflammatory signaling that would confound specialized cell culture outcomes.
High-glucose energy support
4.5 g/L glucose sustains the energy demands of the cell types and protocols targeted by this formulation.
Customization on demand
pH, glucose, salts, and additional component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma purity unavailable from conventional 0.22 µm filtered media.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates and contaminants; protects downstream 0.04 µm membranes and chip geometries from fouling.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) and fine particulates — absent from standard 0.22 µm filtration.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ DCP-DMEMYA1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered DMEM, with a confirmed mycoplasma barrier at every production stage.
© Diagnocine® — DCP-DMEMYA1X
Specialized applications requiring L-Alanyl-Glutamine
FluxMPS™ DCP-DMEMYA1X is designed for research protocols where l-alanyl-glutamine conditions are essential — combined with MPS-grade filtration purity for organ-on-a-chip and microfluidic platform compatibility.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered variant available for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
- Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion circuits
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
Organ-on-a-Chip & MPS
Ultra-clean 0.04 µm filtration prevents microchannel clogging. L-Alanyl-Glutamine formulation delivered particle-free for specialized OoC protocols.
Organ-on-a-Chip Long-Duration Perfusion
FluxMPS™ DCP-DMEMYA1X delivers L-Alanyl-Glutamine conditions with sub-mycoplasma filtration purity for demanding organ-on-a-chip long-duration perfusion platforms.
Bioreactor Continuous Culture
FluxMPS™ DCP-DMEMYA1X delivers L-Alanyl-Glutamine conditions with sub-mycoplasma filtration purity for demanding bioreactor continuous culture platforms.
iPSC Differentiation
FluxMPS™ DCP-DMEMYA1X delivers L-Alanyl-Glutamine conditions with sub-mycoplasma filtration purity for demanding ipsc differentiation platforms.
Glycoprotein Production
FluxMPS™ DCP-DMEMYA1X delivers L-Alanyl-Glutamine conditions with sub-mycoplasma filtration purity for demanding glycoprotein production platforms.
CHO Long-Term Culture
FluxMPS™ DCP-DMEMYA1X delivers L-Alanyl-Glutamine conditions with sub-mycoplasma filtration purity for demanding cho long-term culture platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] High Glucose (4500 mg/L), [+] L-Alanyl-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate |
| Appearance | Red-colored, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| Key feature | L-Alanyl-Glutamine |
| pH USP <791> | 7.4 ± 0.04 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 33 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ required (sodium bicarbonate buffering) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose — L-Alanyl-Glutamine: 33 ingredients verified per lot with CAS numbers for full raw-material traceability. L-Alanyl-L-Glutamine (dipeptide, CAS 39537-23-0) replaces free L-glutamine. Use at the same molar concentration as free L-glutamine (2 mM = equivalent to 292 mg/L L-glutamine). Do not add additional free L-glutamine unless specifically required.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Alanyl-Glutamine | 39537-23-0 | 862.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — eliminates ionic contaminants that would interfere with specialized assay outcomes.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay < 0.05 EU/mL per lot.
Particulate — USP <788> Method 2
Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-DMEMYA1X compares
FluxMPS™ DCP-DMEMYA1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMYA1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG (0.22 µm filtered) |
|---|---|---|---|
| High Glucose DMEM with L-Alanyl-Glutamine — stable nitrogen source for long-duration OoC and bioreactor perfusion | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMYA1X — DMEM High Glucose L-Alanyl-Glutamine.
Supporting literature
Key publications supporting the research applications of FluxMPS™ DCP-DMEMYA1X.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216–4220. doi:10.1073/pnas.77.7.4216
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j