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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEML-PR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Dulbecco's Modified Eagle Medium (DMEM), formulated at low glucose (1.0 g/L) without sodium pyruvate or phenol red, engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. The four-stage train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional sterile-filtered media — supporting unobstructed microchannel flow and low-particulate optical baselines.
- Low Glucose (1.0 g/L) DMEM base formulated without sodium pyruvate and without phenol red, giving researchers full control over carbon-source supplementation and removing phenol red's estrogen-receptor agonist and autofluorescence interference
- Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- Sodium bicarbonate–buffered (3700 mg/L, ≈44 mM); requires an atmosphere of approximately 10% CO₂ to maintain pH 7.4 — not a standard 5% CO₂ incubator setting
- Manufactured under an ISO 13485:2016 quality management system, with final QC and aseptic fill at Diagnocine, Totowa, NJ
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for low trace-metal and total organic carbon background
- Custom pH, glucose concentration, salts, and nutrient adjustments available on request
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- L-Glutamine584 mg/L
- Sodium PyruvateNot included
- pH (USP <791>)7.4
- Osmolality (USP <785>)310 – 350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma (typical diameter 0.2–0.3 µm), subvisible particulates, and endotoxin fragments that can accumulate inside microfluidic channels, foul chip geometries, and add background to sensor readings. FluxMPS™ DCP-DMEML-PR1X was built specifically to address these failure modes.
Microchannel-safe purity
0.04 µm final filtration removes sub-micron particulates that can obstruct channels below 100 µm. USP <788> Method 1 (light obscuration) particulate release limits support safe perfusion across chip geometries.
Total metabolic control
A low glucose (1.0 g/L) base without sodium pyruvate lets researchers define the carbon source, its concentration, and its timing precisely — supporting Warburg-effect and metabolic flux protocols that require a defined starting point.
Ultrapure-grade water
Every batch is prepared using Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696) with controlled trace-metal and total organic carbon content, minimizing the feed-water contribution to background chemistry in sensitive assays.
Low background for imaging
An ultra-low particulate baseline suits confocal, widefield, and biosensor measurements on chip. This phenol red–free formulation removes phenol red's own optical interference; note that riboflavin (0.400 mg/L), present in all DMEM-based media, contributes its own inherent fluorescence and is not removed by filtration.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations, micro-batch manufacturing, and per-lot QC support reproducible cell growth across multi-day perfusion experiments.
Customization on demand
pH, glucose concentration, salts, HEPES, and nutrient composition are adjustable per your protocol. Contact support@diagnocine.com to specify your formulation.
Quadruple-stage filtration system
DCP-DMEML-PR1X is processed through four serial filtration stages — two dedicated 0.1 µm prefilter / 0.04 µm final-filter pairs run in series — reaching a final 0.04 µm polish under aseptic fill conditions.
-
1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates, cell debris, and protein clusters, protecting the first 0.04 µm final-filter cartridge from early fouling.
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2
0.04 µm Final Filtration I
First 0.04 µm pass; retains sub-micron particulates and bacteria and provides mycoplasma-retentive filtration (mycoplasma typical diameter approximately 0.2–0.3 µm).
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3
0.1 µm Prefiltration II
A second, dedicated 0.1 µm prefilter protecting the second 0.04 µm final-filter cartridge and providing redundant particulate and bioburden reduction ahead of the final polish.
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4
0.04 µm Final Filtration II — Polish
Ultimate 0.04 µm polishing pass performed during aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow environment.
Performance vs. conventional media
FluxMPS™ DCP-DMEML-PR1X is manufactured with a validated quadruple-stage filtration train reaching a 0.04 µm final cut-off — a finer pore size than the 0.22 µm membranes used in conventional sterile-filtered DMEM. This finer cut-off supports unobstructed flow in microfluidic channels and chip geometries below 100 µm.
than conventional 0.22 µm
sterile filtration
across two 0.1 µm + 0.04 µm
filter pairs
© Diagnocine® — DCP-DMEML-PR1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEML-PR1X supports demanding cell culture platforms — from single-channel microfluidic chips to multi-organ body-on-a-chip systems — where media purity, metabolic precision, and optical clarity matter.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for automated bioreactor perfusion systems, robotic liquid handlers, and long-duration closed-loop platforms where even trace particulates can affect valves or sensors. This is a distinct grade from the 0.04 µm Microfluidics Suitable product described on this page — see the Grade note above.
- Extended Particulate Exclusion: 0.01 µm filtration targets nanoparticulate aggregates below the resolution of standard QC methods
- Valve & Sensor Protection: Reduces micro-fouling risk to solenoid valves, peristaltic pump tubing, and inline optical sensors
- Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture without in-line filter replacement
Inquiry Required: The 0.01 µm MPS Grade variant is a custom product. Contact support@diagnocine.com to request it for your automated system.
Micro Physiological System (MPS) & Chip
Ultra-clean, 0.04 µm–filtered media supports laminar flow fidelity and reduces microchannel clogging risk in complex multi-organ chip architectures.
Warburg Effect & Metabolic Research
Low glucose (1.0 g/L) base without sodium pyruvate enables precise control of the glycolytic substrate pool, supporting Warburg-phenotype studies.
iPSC-Derived Models
A low-endotoxin release specification (< 0.05 EU/mL) and mycoplasma-retentive filtration make this medium suitable for sensitive iPSC-differentiation protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled DMEM supports HUVEC and primary hepatocyte monolayer integrity, relevant to barrier-function assays and TEER monitoring.
Metabolic Flux Analysis
A defined low-glucose, pyruvate-free formulation provides a clean metabolic background for ¹³C isotope tracing and extracellular flux measurement. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium — this formulation contains sodium bicarbonate (3700 mg/L).
Microscopy & Optical Sensing
Low particulate counts and the absence of phenol red reduce optical background for confocal imaging, biosensor arrays, and TEER measurements on chip.
Analytical release specifications
Every lot is released against the specification matrix below. Available pack sizes: 500 mL, 1000 mL. CoA available on request: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Low Glucose | [-] Phenol Red, [-] Sodium Pyruvate |
| Appearance | Colorless to pale yellow, clear solution (no phenol red indicator) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310 – 350 mOsm/kg H2O |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | Not included |
| Phenol Red | Not included |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification — see §Manufacturing & Compliance) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approximately 10% CO₂ (derived from 44 mM / 3700 mg/L sodium bicarbonate via Henderson–Hasselbalch to maintain pH 7.4; do not use a standard 5% CO₂ incubator setting without verifying pH) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
FluxMPS™ DMEM is a modification of Basal Medium Eagle (BME) containing 4× BME concentrations of amino acids and vitamins, plus glycine, serine, and ferric nitrate. Total ingredients: 31, released per lot. CAS numbers are provided for traceability where known.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| i-Inositol | 87-89-8 | 7.200 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified facilities. Final packaging, QA, and testing are conducted at the Diagnocine R&D and Quality Testing Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
All formulations use Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696) with controlled trace-metal and total organic carbon content.
ISO Class 5 Fill & Finish
Final aseptic fill performed in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity at the point of fill.
Micro-Batch Precision
Small-batch production with full per-lot traceability. Each batch is individually tested and released — no blending of lots. A Certificate of Analysis is issued for every lot.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count. Release limits: NMT 25/mL (≥10 µm) and NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target range: 310 – 350 mOsm/kg H2O.
Documentation & CoA
Full Certificate of Analysis with raw-material traceability, in-process records, and final-release test results available for every lot upon request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEML-PR1X compares
FluxMPS™ DCP-DMEML-PR1X vs. conventional 0.22 µm–filtered DMEM formulations.
| Parameter | DCP-DMEML-PR1X (FluxMPS™) | Conventional DMEM (0.22 µm filtered) |
Standard Alt. DMEM (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Reagent-grade (unspecified) | Reagent-grade (unspecified) |
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Low Glucose | [-] Phenol Red, [-] Sodium Pyruvate | Standard high/low glucose, phenol red included, fixed formulation | Standard high/low glucose, phenol red included, fixed formulation |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle Yes (0.1 µm) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel Not specified | cancel Not specified |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Higher clogging risk | cancel Higher clogging risk |
| Custom formulation | check_circle Available | cancel Fixed formulation | cancel Fixed formulation |
Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEML-PR1X and Microfluidics Suitable DMEM formulations.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip, microfluidic, and metabolic research applications.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
