FluxMPS™ MEM Low Glucose with Earle's Salts w/o Sodium Pyruvate
FluxMPS™ MEM Low Glucose with Earle's Salts w/o Sodium Pyruvate
A quadruple-stage ultra-filtered Minimum Essential Medium formulated with Earle's salts, L-glutamine, and sodium bicarbonate — without sodium pyruvate — for precise low-glucose metabolic control in microfluidic systems, organ-on-chip platforms, and sensitive mammalian cell culture applications requiring sub-mycoplasma purity.
- Quadruple-stage filtration: 0.1 µm × 2 passes + 0.04 µm × 1 pass — sub-mycoplasma final polish (mycoplasma ≥ 0.2 µm excluded)
- Endotoxin: < 1 EU/mL (USP <85> BET); ultrapure Type 1 water (18.2 MΩ·cm) throughout
- Low-glucose formulation (1.0 g/L D-glucose) with Earle's salts for pH and osmotic balance; sodium pyruvate-free
- [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Earle's Salts, [−] Sodium Pyruvate, [−] HEPES, [−] NEAA
- ISO Class 5 (Class 100) aseptic fill; sterility per USP <71> — no bacterial or fungal growth after 14 days
- Manufactured under ISO 13485:2016 QMS; CE-approved supplier; final QA/QC at DiagnoCine R&D in Totowa, NJ, USA
- Custom pH, glucose, HEPES, salts, and nutrient modifications available — contact support@diagnocine.com
- Glucose1.0 g/L (Low)
- L-Glutamine292.0 mg/L (Included)
- Sodium PyruvateNot included
- pH (1X, USP <791>)7.4
- Osmolality (USP <785>)265–305 mOsm/kg H₂O
- Endotoxin (USP <85>)< 1 EU/mL
- Filtration0.1 µm ×2 · 0.04 µm ×1
- Storage2–8 °C, protected from light
- Shelf Life12 months
- ShippingAmbient (cold pack on request)
Engineered where standard media fails
Conventional 0.22 µm-filtered MEM-grade media leaves behind subvisible particulates, mycoplasma fragments, and aggregates that clog microchannels, corrupt metabolic assay signals, and undermine reproducibility in organ-on-chip and high-sensitivity cell culture systems. FluxMPS™ MEM applies a four-stage progressive-filtration architecture — culminating in a final 0.04 µm sterile polish — to deliver a purity level approximately 5× cleaner by particulate count than standard media, with zero compromise to formulation fidelity.
Microchannel-Safe Purity
Final 0.04 µm filtration exceeds USP <788> particulate thresholds — prevents aggregate accumulation that blocks microfluidic channels and distorts flow profiles.
Total Metabolic Control
Low-glucose (1.0 g/L) Earle's salt formulation without sodium pyruvate enables precise Warburg-effect studies and carbon-source-defined metabolic experiments free from pyruvate interference.
Sub-Mycoplasma Polishing
0.04 µm final filter retains the smallest mycoplasma types (≥ 0.2 µm) — delivering ultra-low particulate, microchannel-safe media without UV or antibiotic treatment.
Ultrapure Type 1 Water
All formulation performed with 18.2 MΩ·cm ultrapure Type 1 water — eliminating ionic contaminants that shift osmolality and alter cell signaling in sensitive assay systems.
Regulatory-Grade QMS
Manufactured under ISO 13485:2016 QMS with CE-approved supplier network; 21 CFR Part 820 (cGMP) aligned. Every lot released with endotoxin, sterility, pH, osmolality, and cultural-response testing.
Customization on Demand
pH, glucose concentration, Earle's vs. Hank's salts, HEPES addition, NEAA inclusion, and nutrient modifications available on request — support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ MEM is the only ready-to-use 0.04 µm-polished MEM at this purity level. Each lot passes through four sequential filtration stages in an ISO Class 5 (Class 100) cleanroom environment — delivering validated sub-mycoplasma sterility and ultra-low particulate burden for the most demanding microfluidic and high-sensitivity cell culture applications.
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1
0.1 µm Pre-filtration I
Removes large particulates and aggregates from raw formulation — extends downstream filter service life and reduces bioburden load entering sterile stages.
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2
0.04 µm Pre-filtration II
Fine particulate retention, bacteria removal, and mycoplasma capture — first application of the 0.04 µm membrane ensures near-complete biological burden elimination before sterile filling.
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3
0.1 µm Sterile-filtration I
Second-pass 0.1 µm redundancy confirms sterility of bulk media pool — any particulates released from upstream membranes are captured before final polish.
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4
0.04 µm Sterile-filtration II — Final Polish
Ultimate polish under ISO Class 5 aseptic fill conditions. Delivers the lowest achievable particulate count for ready-to-use liquid MEM — microchannel-safe and USP <788> compliant.
Performance vs. conventional media
Conventional 0.22 µm-filtered MEM allows passage of subvisible particles and mycoplasma-sized fragments that accumulate in microfluidic channels, interfere with optical biosensors, and corrupt metabolic flux measurements. FluxMPS™ MEM's 0.04 µm final filter delivers a fundamentally cleaner baseline — enabling reproducible, artifact-free data in the most sensitivity-critical experiments.
Where FluxMPS™ MEM performs
FluxMPS™ MEM Low Glucose with Earle's Salts supports a broad spectrum of research applications that demand both precise low-glucose metabolic control and sub-mycoplasma media purity. From microfluidic organ-on-chip platforms to Warburg-effect metabolomics, this formulation delivers consistent, reproducible results across sensitive biological systems.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant of FluxMPS™ MEM is available for automated bioreactor and liquid-handling robotics applications where even 0.04 µm-level particulates may risk valve fouling, sensor drift, or perfusion inconsistency over extended culture runs.
- Total Particulate Exclusion: 10 nm filtration removes nanoscale aggregates incompatible with precision fluidic systems
- Valve & Sensor Protection: Eliminates particulate-driven fouling in microvalves, pressure transducers, and optical sensors
- Extended Perfusion Stability: Sustained ultra-clean media supply over multi-day perfusion culture without filter replacement
Inquiry Required: The 0.01 µm (10 nm) ultra-filtered grade is produced in small batches and is not listed as a standard catalog item. Contact support@diagnocine.com to request this variant.
Micro Physiological System (MPS) & Chip
Ultra-low particulate MEM for organ-on-chip and tissue-chip platforms requiring particle-free, mycoplasma-safe media compatible with PDMS and glass microchannel surfaces.
Warburg Effect & Metabolic Research
Low-glucose, pyruvate-free formulation ideal for isolating glycolytic flux from oxidative phosphorylation — enables clean Warburg-effect modeling without confounding carbon sources.
iPSC-Derived Models
Earle's salt formulation supports delicate iPSC-derived cell types sensitive to osmotic fluctuation and metabolic perturbation during differentiation and functional assay phases.
Endothelial & Primary Cells
MEM with Earle's salts supports primary endothelial and hepatocyte cultures — used in fibroblast culture, virus propagation, HeLa, BHK-21, 293, HEP-2, HT-1080, and MCF-7 applications.
Metabolic Flux Analysis
Pyruvate-free, defined low-glucose base medium enables isotope-tracing metabolic flux experiments without background from undefined carbon sources in the media.
Microscopy & Optical Sensing
Sub-mycoplasma purity and ultra-low particulate burden preserve optical clarity in live-cell imaging, biosensor, and TEER measurement setups where background scatter must be minimized.
Lot-release analytical parameters
Every lot of FluxMPS™ MEM Low Glucose with Earle's Salts is released against the following analytical criteria. Values represent specification limits; certificates of analysis with lot-specific measured values are available on request.
| Parameter | Specification |
|---|---|
| Formulation | MEM, [+] Earle's Salts, [+] Low Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [−] Sodium Pyruvate, [−] HEPES, [−] NEAA |
| Appearance | Orange-to-Red, clear solution (phenol red indicator) |
| pH USP <791> | 7.4 at 1X |
| Osmolality USP <785> | 265–305 mOsm/kg H₂O |
| D-Glucose | 1,000 mg/L (1.0 g/L) |
| L-Glutamine | 292.0 mg/L |
| Sodium Pyruvate | Not present |
| Phenol Red | 11.0 mg/L (sodium salt) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | NMT 1 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | Prevented by 0.04 µm filtration (USP <63> equivalent) |
| Particulate ≥ 10 µm USP <788> M2 | Compliant |
| Particulate ≥ 25 µm USP <788> M2 | Compliant |
| Water Purity | Ultrapure Type 1 (18.2 MΩ·cm) |
| Manufacturing Std. | ISO 13485:2016; CE-approved supplier |
| Fill Environment | ISO Class 5 (Class 100) aseptic |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2–8 °C, away from bright light |
| Freeze-Thaw | Do not freeze |
| Shelf Life | 12 months from manufacture date |
| Shipping Condition | Cold pack |
| CO₂ Requirement | 5% CO₂ atmosphere recommended with Earle's salts formulation |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Cell culture / reagent grade or higher |
| Traceability | Full lot-level documentation; CoA available on request |
| Manufacturing QMS ISO 13485 | ISO 13485:2016 certified supplier; CE-approved |
| Regulatory Alignment | 21 CFR Part 820 (cGMP) aligned; USP <85>, <71>, <785>, <788>, <791> |
| Production Method | Compounding + quadruple-stage membrane filtration; ISO Class 5 fill |
| Intended Use | Research Use Only (RUO). Not for clinical, diagnostic, or therapeutic use. |
Full composition (mg/L)
Complete formulation at 1X concentration. Composition data reproduced character-for-character from lot specification. Every ingredient is released per-lot against this reference. Customization of any component is available on request — contact support@diagnocine.com.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2200.00 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 31.300 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 1007-42-7 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt | 69847-55-8 | 51.900 |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Niacinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| i-Inositol | 87-89-8 | 2.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
Manufacturing standards & compliance
FluxMPS™ MEM is produced by ISO 13485:2016-certified, CE-approved manufacturing partners — with all final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center. Customization and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering design controls, supplier qualification, production, and post-market surveillance — aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
All formulation uses 18.2 MΩ·cm ultrapure water throughout — eliminating ionic, organic, and microbial contaminants at the water source.
ISO Class 5 Fill & Finish
Final aseptic fill performed in ISO Class 5 (Class 100) cleanroom environment — ensures particle and microorganism exclusion at the point of packaging.
Micro-Batch Precision
Small-batch compounding with full lot traceability — each batch is individually tested for pH, osmolality, endotoxin, sterility, and cultural response before release.
Endotoxin — USP <85> BET
Lot-release endotoxin testing by Limulus Amebocyte Lysate (LAL) method per USP <85>. Specification: Not More Than (NMT) 1 EU/mL.
Particulate Matter — USP <788> Method 2
Light obscuration particle count testing per USP <788> Method 2 — confirms ultra-low particulate burden consistent with microfluidic-grade media.
Sterility — USP <71>
14-day incubation sterility test per USP <71> specification. No bacterial or fungal growth observed for released lots.
Cultural Response Testing
Growth promotion capacity assessed qualitatively (cell morphology) and quantitatively (cell count vs. control medium) for each production lot.
FluxMPS™ vs. conventional MEM
Standard 0.22 µm-filtered MEM formulations from conventional suppliers leave subvisible particulates and mycoplasma-sized fragments that accumulate in sensitive systems. FluxMPS™ MEM addresses these gaps with a validated four-stage architecture.
| Feature | FluxMPS™ MEM (DCP-MEM-PN1X) | Standard MEM (0.22 µm-filtered) |
|---|---|---|
| Final filter pore size | 0.04 µm (40 nm) | 0.22 µm |
| Filtration stages | 4 stages (0.1 µm × 2 + 0.04 µm × 2) | 1 stage |
| Mycoplasma prevention | check_circle 0.04 µm retention (USP <63> equiv.) | cancel Not addressed |
| Particulate count | check_circle ~5× lower vs. 0.22 µm media | cancel Conventional level |
| ISO Class 5 fill | check_circle Yes | cancel Not specified |
| ISO 13485:2016 QMS | check_circle Certified supplier | cancel Varies |
| Endotoxin spec | < 1 EU/mL (lot-tested USP <85>) | Typically ≤ 1–5 EU/mL, not always lot-tested |
| Ultrapure Type 1 water | check_circle 18.2 MΩ·cm | cancel Not specified |
| Microfluidic / OoC grade | check_circle Validated | cancel Not validated |
| Customization | check_circle pH, salts, supplements, glucose | cancel Fixed formulation |
Frequently asked questions
Supporting literature
The following publications provide foundational context for MEM formulation, Earle's salt buffering, microfluidic cell culture media requirements, and USP-grade media quality standards referenced in this product description.
- Eagle H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955;89(3):362–364. doi:10.3181/00379727-89-21807
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432–437. doi:10.1126/science.130.3373.432
- Bhatt DL, et al. Microfluidic organ-on-a-chip platforms for drug discovery. Nat Rev Drug Discov. 2022. Media purity requirements for organ-on-chip systems reviewed.
- United States Pharmacopeia. USP <71> Sterility Tests; USP <85> Bacterial Endotoxins Test; USP <788> Particulate Matter in Injections; USP <791> pH; USP <785> Osmolality. USP-NF. Current edition.
