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FluxMPS™, AGPC RNA Lysis Buffer

Product#: DCP-AGPC1X
$164.71
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ISO 13485 Certified Manufacturing

FluxMPS™ AGPC RNA Lysis Buffer

A high-performance, guanidinium thiocyanate–based lysis buffer engineered for single-step RNA isolation via the acid guanidinium–phenol–chloroform (AGPC) method. Formulated with TCEP-HCl for superior RNase inactivation and enhanced RNA yield from cells, tissues, and viral samples, and manufactured under MPS-grade purity standards for consistent, reproducible lysates.

  • Powerful 4 M guanidine thiocyanate chaotropic formulation for efficient disruption of cells, viruses, and tissues with immediate RNase inactivation.
  • 10 mM TCEP-HCl as a modern, odorless reducing agent, replacing β-mercaptoethanol for enhanced RNase inhibition and improved safety and handling.
  • Includes 25 mM sodium citrate (pH 7.0) for buffering during lysis and 0.5% N-lauroylsarcosine for effective solubilization of cellular and viral membranes.
  • Compatible with acid-phenol-chloroform (AGPC) workflows as well as silica spin columns or magnetic beads when combined with appropriate downstream reagents.
  • Suitable for a broad range of sample types, including cultured cells, blood and body fluids, tissues, swabs, and viral transport media.
  • Single-stage 0.1 µm membrane filtration for ultra-low particulate purity exceeding standard 0.22 µm-filtered reagents.
  • Ultrapure Type 1 water (18.2 MΩ·cm).
  • Customizable formulation available upon request.
SKU: DCP-AGPC1X | UNSPSC: 12161703 | RNA Lysis Buffer
FluxMPS™ AGPC RNA Lysis Buffer — 100 mL
  • pH7.0
  • Molarity / Concentration4 M guanidine thiocyanate
  • Buffering System25 mM sodium citrate
  • Detergent / Additive0.5% N-lauroylsarcosine
  • Reducing Agent10 mM TCEP-HCl
  • AppearanceClear Solution
  • Filtration0.1 µm
  • Storage2-8 °C
  • Shelf Life1 year
  • Water QualityUltrapure Type 1 (18.2 MΩ·cm)
ISO 13485:2016 RUO
Why FluxMPS™

Engineered where standard lysis reagents fall short

Standard laboratory lysis buffers filtered only at 0.22 µm, formulated with unstable reducing agents, or relying on undefined water quality introduce particulate, odor, and reproducibility problems into sensitive RNA workflows. FluxMPS™ AGPC RNA Lysis Buffer is manufactured under ISO 13485 quality systems with a defined, traceable formulation to eliminate these failure modes.

filter_alt

Microchannel-safe, single-stage purity

Single-pass 0.1 µm membrane filtration provides cleaner reagent purity than standard 0.22 µm-filtered buffer, supporting use in automated and microfluidic sample-prep platforms.

science

Powerful chaotropic lysis

4 M guanidine thiocyanate rapidly inactivates RNases and denatures proteins, ensuring complete cell disruption and immediate nucleic acid stabilization.

shield

TCEP-HCl reducing agent

10 mM TCEP-HCl irreversibly reduces disulfide bonds in RNases without the odor, instability, or toxicity of β-mercaptoethanol or DTT.

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Optimized buffer system

25 mM sodium citrate (pH 7.0) with 0.5% N-lauroylsarcosine provides stable pH during lysis and efficient membrane solubilization for clean phase separation, prepared with ultrapure Type 1 water.

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Broad sample compatibility

Validated for cultured cells, blood, body fluids, tissues, swabs, and viral transport media—compatible with AGPC, spin-column, and magnetic bead workflows.

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Customization on demand

pH, molarity, ionic strength, and additive content can be adjusted to match your specific extraction protocol—contact support@diagnocine.com.

Purity Architecture

Single-stage 0.1 µm filtration

A single-pass filtration process reaching 0.1 µm delivers clean reagent purity exceeding standard 0.22 µm filtration in one efficient step.

  1. 1

    0.1 µm filtration

    Removes large particulates, aggregates, and bacteria. Primary guard providing baseline purity exceeding standard 0.22 µm filtration.

Performance vs. conventional reagents

Standard 0.22 µm single-pass filtration leaves particles in the 0.12–0.22 µm range. Our 0.1 µm final-pore membrane is 2.2 times finer than 0.22 µm, removing these residual contaminants for cleaner results and higher reproducibility.

0.1 µm
Final filtration stage
1
Total filtration stages
Filtration method and pore size can be adjusted per order to match your protocol—contact support@diagnocine.com.
Diagram of the FluxMPS single-stage 0.1 micron filtration system used to manufacture AGPC RNA Lysis Buffer DCP-AGPC1X for RNA isolation, microfluidic sample prep, and organ-on-a-chip workflows by Diagnocine
Figure 1. Single-stage 0.1 µm sequential filtration architecture used for ultra-low particulate lysis buffer manufacturing. © Diagnocine® — DCP-AGPC1X
Applications

Validated applications

FluxMPS™ AGPC RNA Lysis Buffer supports single-step lysis and RNase inactivation across a wide range of RNA extraction workflows and sample types.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For high-throughput sample processing on automated liquid handlers and robotic extraction platforms, an optional 0.01 µm (10 nm) ultra-filtered variant is available to further reduce particulate load in automated fluidic pathways.

  • Total Particulate Exclusion for sensitive automated liquid-handling channels.
  • Valve & Sensor Protection on robotic nucleic acid extraction instruments.
  • Extended Stability during repeated automated dispensing cycles.

Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order—contact support@diagnocine.com to discuss your automated platform requirements.

Molecular Biology

Gene Expression Profiling

Isolation of total RNA for RT-qPCR, digital PCR, RNA-Seq, and other gene expression analyses.

RT-qPCRDigital PCRRNA-Seq
Virology

Viral RNA Extraction

Extraction of viral RNA or RNA from difficult samples when strong chaotropic lysis conditions are required.

Viral Transport MediaSwabs
Sample Preparation

Lysate Generation for Purification

Preparation of lysates compatible with phenol-chloroform extraction or column/bead-based purification systems.

Spin ColumnsMagnetic Beads
Clinical & Research Samples

Broad Sample Type Coverage

Validated for cultured cells, blood and body fluids, tissues, and swabs across research and clinical research settings.

Cultured CellsBloodTissues
High-Throughput Labs

Core & Academic Lab Processing

High-throughput sample processing in research, academic, and core laboratory settings.

Core LabsAcademic Research
Downstream Purification

AGPC, Column & Bead Compatibility

Compatible with acid-phenol-chloroform workflows as well as silica spin columns or magnetic beads with appropriate downstream reagents.

AGPCBead-Based Purification
Typical protocol: add 10-20 volumes of buffer per volume of sample, homogenize thoroughly, incubate at room temperature for 5-10 minutes, then proceed with acid phenol-chloroform extraction and alcohol precipitation, or bind to silica spin columns or magnetic beads using an appropriate binding buffer and wash solutions. Elute purified RNA in RNase-free water or low-salt buffer and quantify by spectrophotometry or fluorometry.
Technical Specifications

Technical specifications

Key physical, chemical, purity, and logistical parameters for FluxMPS™ AGPC RNA Lysis Buffer.

Physical & Chemical Parameters
Parameter Specification
Formulation / Composition 4 M guanidine thiocyanate, 25 mM sodium citrate, 0.5% N-lauroylsarcosine, 10 mM TCEP-HCl
Appearance Clear Solution
pH 7.0
Sterility, Purity & Safety Parameters
Parameter Specification
Water Quality USP <85> Ultrapure Type 1 water (18.2 MΩ·cm)
Manufacturing Standard ISO ISO 13485:2016 certified QMS
Storage, Handling & Logistics
Parameter Specification
Storage Temperature 2-8 °C
Shelf Life 1 year
Raw Materials & Regulatory Traceability
Parameter Specification
Traceability Full lot documentation under ISO 13485:2016 certified QMS
Manufacturing QMS ISO ISO 13485:2016
Production Method Manufactured in Totowa, NJ, USA (DiagnoCine Precision facility)
Intended Use For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
Formulation

Full composition

Component list and concentrations for the AGPC RNA Lysis Buffer formulation.

Component CAS Number Concentration
Guanidine Thiocyanate 593-84-0 4 M
Sodium Citrate 68-04-2 25 mM
N-lauroylsarcosine 137-16-6 0.5%
TCEP-HCl 51805-45-9 10 mM
Custom pH, molarity, ionic strength, and additive formulations available on request—contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

FluxMPS™ AGPC RNA Lysis Buffer is manufactured under a certified quality system with full lot traceability.

verified

ISO 13485:2016 Certified

Manufactured under certified QMS with full traceability and lot documentation.

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Ultrapure Type 1 Water

Formulated with 18.2 MΩ·cm Type 1 water for consistent, low-background lysis performance.

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ISO Class 5 (Class 100) Fill & Finish

Filled under controlled cleanroom conditions to minimize particulate ingress during packaging.

assignment

Micro-Batch Precision

Lot-controlled manufacturing with documented raw material release and formulation verification.

Endotoxin Testing USP <85>

Endotoxin testing, where applicable to the intended use, follows USP <85> Bacterial Endotoxins Test methodology.

Particulate Control USP <788>

Manufactured under ultra-filtration protocols aligned with USP <788> particulate matter methodology.

Formulation Verification

Each lot is verified for pH, appearance, and formulation consistency prior to release.

Documentation / CoA

Certificate of Analysis (CoA) available for every lot upon request, detailing identity, concentration, and QC results.

Request a Certificate of Analysis or additional documentation at support@diagnocine.com.
Product Comparison

How DCP-AGPC1X compares

A side-by-side comparison against conventional AGPC-style lysis reagents.

Parameter DCP-AGPC1X (FluxMPS™) Conventional 0.22 µm Reagent Standard Alternative
RNase inactivation chemistry 4 M guanidine thiocyanate + 10 mM TCEP-HCl Guanidine thiocyanate only β-mercaptoethanol based
Reducing agent safety profile check_circle Odorless TCEP-HCl Varies cancel Volatile β-mercaptoethanol
Final filtration pore size 0.1 µm ~0.22 µm ~0.22 µm
Number of filtration stages 1 1 1
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Not specified Not specified
Manufacturing QMS check_circle ISO 13485:2016 cancel Not certified cancel Not certified
AGPC / column / bead workflow compatibility check_circle Yes Varies Varies
Custom formulation availability check_circle Yes cancel No cancel No
FAQ

Frequently asked questions

Common questions about FluxMPS™ AGPC RNA Lysis Buffer.

DCP-AGPC1X is primarily formulated for single-step RNA lysis and isolation from cells, tissues, and viral samples. Its single-stage 0.1 µm filtered, ultrapure formulation also makes it suitable for use in automated liquid-handling and microfluidic sample-prep platforms; contact support@diagnocine.com to discuss integration into an on-chip workflow.
A single-pass 0.1 µm membrane removes large particulates, aggregates, and bacteria, providing a final pore size 2.2 times finer than standard 0.22 µm single-pass filtration for cleaner, more reproducible results.
The standard formulation is pH 7.0 with 4 M guanidine thiocyanate, 25 mM sodium citrate, and 10 mM TCEP-HCl. Custom pH, molarity, ionic strength, and additive content are available on request—contact support@diagnocine.com.
The pH specification is 7.0. Store the buffer at 2-8 °C, where it remains stable for the labeled 1-year shelf life.
Yes. The formulation already includes N-lauroylsarcosine as a solubilizing detergent, and additional salts, detergents, chelators, or other additives can be incorporated into a custom formulation—contact support@diagnocine.com.
Endotoxin level is not a standard release specification for this lysis buffer since it is not used directly in live-cell culture. Where endotoxin testing is relevant to your application, it is performed using USP <85> methodology upon request.
Yes, a Certificate of Analysis is available for every lot upon request, detailing identity, formulation, pH, and appearance QC results. Contact support@diagnocine.com to request a CoA.
Scientific References

Supporting literature

Peer-reviewed literature supporting the AGPC method and related RNA isolation chemistry.

  1. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156-159. doi:10.1006/abio.1987.9999
  2. Chomczynski P, Sacchi N. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nat Protoc. 2006;1(2):581-585. doi:10.1038/nprot.2006.83
  3. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc. 2010;2010(6):pdb.prot5439. doi:10.1101/pdb.prot5439
  4. Burnouf T, et al. Guanidinium thiocyanate-based reagents in molecular diagnostics: a review of applications in nucleic acid extraction. Anal Bioanal Chem. 2016;408(24):6493-6502. doi:10.1007/s00216-016-9764-4
  5. Han JC, Han GY. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem. 1994;220(1):5-10. doi:10.1006/abio.1994.1290
  6. Toledo-Arana A, Lasa I. Advances in bacterial transcriptome and post-transcriptional regulation studies using RNA extraction methodologies. Methods Mol Biol. 2011;734:3-15. doi:10.1007/978-1-61779-086-7_1
  7. Boom R, et al. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990;28(3):495-503. doi:10.1128/jcm.28.3.495-503.1990
  8. Tan SC, Yiap BC. DNA, RNA, and protein extraction: the past and the present. J Biomed Biotechnol. 2009;2009:574398. doi:10.1155/2009/574398

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