Flamma® 675 Azide
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 675 Azide
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 675/691 nm
2. High extinction coefficient: ≥ 232,000 M?¹cm?¹
3. Azide reactive group connected through an amino propyl linkage
4. Spectral similarity to Alexa 680, Cy5.5, IRDye 680LT, and DyLight 680
Applications:
1. Cellular imaging and detection
2. Nucleotide functionalization
3. Bioorthogonal labeling in complex biological environments
4. In vivo imaging studies
5. Deep-tissue imaging
6. Metabolic labeling
7. Protein and peptide modification
Advantages:
1. NIR spectral properties allow for deep tissue penetration and minimal autofluorescence
2. Efficient excitation using a 633 nm laser line
3. Forms 1,4-disubstituted 1,2,3-triazoles through CuAAC reactions
4. Minimal interference with native biochemical processes
5. High sensitivity for detecting low-abundance biological structures
6. Improved signal-to-noise ratios in complex biological samples
7. Compatibility with existing imaging systems
Flamma® Fluors 675 Azide features an azide reactive group connected to the dye through an amino propyl linkage. This design facilitates the formation of 1,4-disubstituted 1,2,3-triazoles when coupled with an alkyne in living systems. The CuAAC reaction occurs efficiently without interfering with native biochemical processes, making it suitable for sensitive biological applications.
- Fluorophore: Flamma® Fluors 675
- Reactive group: Azide
- Excitation/Emission Max.(nm): 674/691
- Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT
- Extinction coefficient: ≥ 130,000 cm-1M-1
- CF280: 0.1
- Appearance: Blue Solid
- Molecular Weight: 1027.21 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWZ1001 | Flamma® 496 Azide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWZ1122 | Flamma® 552 Azide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWZ1415 | Flamma® 581 Azide | 581 | 596 | Alexa594, DyLight594 |
| PWZ1215 | Flamma® 648 Azide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWZ1515 | Flamma® 675 Azide | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWZ1301 | Flamma® 749 Azide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWZ1603 | Flamma® 774 Azide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Catalyst Utilization: The use of copper(I) as a catalyst enables efficient coupling reactions.
- Biocompatibility: The reaction can be performed under physiological conditions without disrupting cellular functions.
- High Specificity: The formation of triazoles provides high specificity in labeling biomolecules.
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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