Epidermal Growth Factor, Human, Active EGF (5 x 50 µg)
Epidermal Growth Factor, Human, Active <EGF>
Cat. No. FNK-03-001-5
Store at -20°C
Size 5x50µg
Description
EGF is a polypeptide growth hormone which plays an important role in the regulation of growth, proliferation, and differentiation of a wide range of cells by binding to its receptor EGFR (epidermal growth factor receptor). Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. The full-length human EGF was expressed in E.coli and highly purified by the propriety chromatographic procedures. This product has the same amino acid sequence with the mature human EGF (6.35 kDa) and has no tag attached.
Fig. 1. SDS-PAGE of human EGF
Applications
1. EGF is commonly used as a supplement in serum-free or reduced serum media for culture of mammalian cells.
2. Studies of the human EGF receptor, transmembrane signaling and protein phosphorylation
3. Western blotting control for anti-EGF antibodies
4. Acceleration of the healing process of epidermis damage on skin, cornea, etc.
5. Widely used in cosmetics, such as whitening, anti-crinkle, anti-aging, etc.
Specification
Activity
The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96 AQueous Assay; Promega) with NHEK cells (normal human epidermal keratinocytes) was < 0.1 ng/ml, corresponding to a specific activity of < 1 x 107 units/mg.
Purity
>95% as determined by SDS-PAGE (CBB staining)
Form
0.74 mg/ml in PBS (10mM Na-phosphate, 150mM NaCl) pH7.2, 50% glycerol, filter-sterilyzed
Storage
-20℃ (for long-term storage, -70℃)
References
- Carpenter G & Cohen S (1990) “Epidermal growth factor.” J Biol Chem 265:7709-7712 PMID: 2186024
- Cohen S, Carpenter G (1975) “Human epidermal growth factor: Isolation and chemical and biological properties.” Proc Natl Acad Sci USA 72: 1317-1321 PMID: 1055407
