Human Keratinocyte Growth Factor (KGF/FGF7), active
Product#: FNK-03-005-5
$945.36
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Human Keratinocyte Growth Factor (KGF/FGF7), active
Cat. No. FNK-03-005-5
Store at -20°C
Size 5 x 50µg
Description
Keratinocyte Growth Factor (KGF), also known as Fibroblast Growth Factor 7 (FGF-7), is a member of fibroblast growth factor (FGF) family. Although FGF-7 has heparin binding activity similar to FGF-1, its mitogenic activity is predominantly exhibited in keratinocytes. It is not effective to fibroblasts and endothelial cells. The human FGF-7 lacking the signal sequence (1-31 aa) was expressed in E. c oli and purified by the chromatographic procedures. This product is an intact enzyme without tag with 19 kDa size (Fig).
Fig. SDS-PAGE of human FGF-7
- Mitogen for epithelial cells
- Western blot control for anti-FGF-7 antibodies
- Acceleration of wound healing is implied.
- Acceleration of hair development is implied
The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96, Promega) with human keratinocyte JCRB141cells was < 10 ng/ml.
Purity >95% as determined by SDS-PAGE (CBB staining)
Form 1.0 mg / ml in PBS (10mM Na-phosphate, 150mM NaCl) pH7.2, 50% glycerol, filter-sterilized
Storage -20℃ (long period, -80℃)
Data Link
GeneID: 2252,
Gene Sequence: M60828.1,
Amino Acid Sequence: P21781
References
Gene Sequence: M60828.1,
Amino Acid Sequence: P21781
- Rubin JS et al.(1989) “Purification and characterization of a newly identified growth factor specific for epithelial cells.” Proc Natl Acad Sci USA 86: 802-806 PMID: 2915979
- Aaronson SA et al. (1991) ”Keratinocyte growth factor. A fibroblast growth factor family member with unusual target cell specificity.” Ann NY Acad Sci 638:62-77 PMID: 1664700
