Earle's Balanced Salt Solution w/ Sodium bicarbonate and Phenol red w/o Calcium and Magnesium: 1X
Cat No.: DCP-EBSSBR-CM1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] Phenol Red, [+] Sodium Bicarbonate
[-] Calcium Chloride, [-] Magnesium Sulfate
Description:
Earle's balanced salt solution (EBSS) is a fundamental isotonic saline solution formulated by W.R. Earle in 1943. This solution provides an essential environment that maintains cellular structural and physiological integrity in laboratory settings. The carefully balanced composition of inorganic salts, combined with glucose as an energy source, makes EBSS a critical component in various cell culture applications. This specially formulated Earle's Balanced Salt Solution (EBSS) provides an optimal physiological environment for cell maintenance while offering the versatility of a calcium and magnesium-free formulation. Ready-to-use at 1X concentration, this liquid solution combines essential inorganic salts with buffer components for reliable performance in your cell culture applications.
Advantages:
- Convenience: Ready-to-use 1X formulation eliminates preparation time and reduces risk of formulation errors
- Reliability: Manufactured to strict specifications for consistent performance
- Versatility: Supports multiple laboratory applications requiring calcium and magnesium-free conditions
- Quality Assurance: Each lot tested for pH, osmolality, and sterility to ensure optimal performance
Applications:
- Cell Dissociation Protocols: Perfect for washing cells before trypsinization or enzymatic dissociation, as the absence of divalent cations enhances the efficiency of cell detachment
- EDTA-Based Cell Passaging: Complements EDTA treatments by preventing interference from calcium and magnesium ions
- Specialized Cell Culture Applications: Supports experiments where calcium and magnesium may interfere with specific cellular responses or signaling pathways
- Cell Washing: Provides gentle yet effective removal of serum components before experimental procedures
Composition:
| Ingredients |
mg/L |
| Sodium Phosphate Monobasic |
122.00 |
| Potassium Chloride |
400.000 |
| Sodium Bicarbonate |
2200.000 |
| Sodium Chloride |
6800.000 |
| D-Glucose |
1000.000 |
| Phenol Red Sodium Salt |
11.000 |
Quality Control:
- Appearance: Red colored, clear solution.
- pH: 7.6
- Osmolality: Approximately 265-305 mOsm/kg H2O
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 2-8 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
