Earle's Balanced Salt Solution w/ Sodium bicarbonate w/o Calcium, Magnesium and Phenol red: 1X
Cat No.: DCP-EBSSB-CMR1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] Sodium Bicarbonate
[-] Phenol Red, [-] Calcium Chloride, [-] Magnesium Sulfate
Description:
Earle's balanced salt solution (EBSS) is a fundamental isotonic saline solution formulated by W.R. Earle in 1943. This solution provides an essential environment that maintains cellular structural and physiological integrity in laboratory settings. The carefully balanced composition of inorganic salts, combined with glucose as an energy source, makes EBSS a critical component in various cell culture applications. This specialized formulation of Earle's Balanced Salt Solution (EBSS) is designed to provide optimal support for cell culture applications requiring a calcium and magnesium-free environment with enhanced optical clarity. Supplied as a ready-to-use 1X liquid solution, this EBSS variant offers convenience and reliability for your research needs.
Advantages:
- Cell washing prior to dissociation procedures
- Short-term cell maintenance during experimental manipulations
- Specialized protocols requiring absence of calcium and magnesium
- Imaging and fluorescence-based assays benefiting from lack of phenol red
Applications:
- Versatility: Supports various cell culture applications
- Optical Clarity: Phenol red-free formulation enhances imaging quality
- Convenience: Ready-to-use format saves time and reduces preparation errors
- Compatibility: Ideal for use with chelating agents and in calcium-sensitive experiments
Composition:
| Ingredients |
mg/L |
| Sodium Phosphate Monobasic |
122.000 |
| Potassium Chloride |
400.000 |
| Sodium Bicarbonate |
2200.000 |
| Sodium Chloride |
6800.000 |
| D-Glucose |
1000.000 |
Quality Control:
- Appearance: Red colored, clear solution.
- pH: 7.6
- Osmolality: Approximately 265-305 mOsm/kg H2O
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 2-8 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
