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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEMLH-PR1X is a Microfluidics Suitable, ultra-filtered DMEM Low Glucose + HEPES formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (Low, 1 g/L) | [-] Sodium Pyruvate, [-] Phenol Red. The 25 mM HEPES buffer (pKa 7.3 at 37°C) supplements the sodium bicarbonate buffer system, extending pH stability during open-bench handling and in open-top microfluidic chip formats.
- 0.04 µm final nano-filtration — ultra-low particulate and mycoplasma-retentive purity for microfluidic channels below 100 µm
- Quadruple-stage filtration train: Prefiltration I (0.1 µm) → Final filtration I (0.04 µm) → Prefiltration II (0.1 µm) → Final filtration II (0.04 µm, polish)
- Endotoxin < 0.05 EU/mL (LAL, USP <85>), released per manufacturing batch with full Certificate of Analysis
- Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (Low, 1 g/L) | [-] Sodium Pyruvate, [-] Phenol Red
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 aseptic fill
- 25 mM HEPES buffer (pKa 7.3 at 37°C) supplements sodium bicarbonate buffering for stability during open-bench handling
- Custom pH, salts, and nutrient adjustments available on request
- Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (Low) | [-] Sodium Pyruvate, [-] Phenol Red
- AppearancePale yellow to colorless, clear solution (phenol red-free)
- pH (USP <791>)7.4
- Osmolality (USP <785>)310 – 350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Total ingredients32
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma-scale particles, subvisible particulates, and process-derived debris that can accumulate in microfluidic channels and interfere with optical sensors. FluxMPS™ is built to reduce these failure modes with a four-stage sub-0.04 µm filtration train. The 25 mM HEPES buffer supplements the sodium bicarbonate system, adding pH stability for open-top chip architectures and brief atmospheric handling.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion across a wide range of chip geometries.
Total metabolic control
Selective inclusion/exclusion of pyruvate, phenol red, bicarbonate, and HEPES gives precise nutrient and buffer definition for your protocol.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) produced under tight trace-metal and total organic carbon (TOC) control for consistent, reproducible culture performance.
Low background for imaging
Ultra-low particulate baseline (0.04 µm final filtration) supports confocal imaging and biosensor applications; note that riboflavin in this formulation contributes native fluorescence typical of DMEM-based media.
Rich, stable nutrient profile
32 ingredients verified per lot; micro-batch production with full raw-material traceability.
Customization on demand
pH, glucose, salts, HEPES, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
-
1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates, cell debris and protein aggregates; protects the first 0.04 µm final filter.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains mycoplasma-scale particles (0.2–0.3 µm) and sub-micron aggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter protecting the second 0.04 µm final filter cartridge; provides full redundancy across the train.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill and finish under ISO Class 5 conditions.
Performance vs. conventional media
© Diagnocine® — DCP-DMEMLH-PR1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMLH-PR1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. The integrated 25 mM HEPES buffer makes it particularly suited to open-top microfluidic devices and brief atmospheric handling.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
Ultra-clean 0.04 µm–filtered media reduces the risk of microchannel clogging in complex multi-organ chip architectures.
CHO & Mammalian Cell Culture
Suited to CHO, cancer, and primary cell lines in serum-free or low-serum culture conditions.
iPSC-Derived Models
Low endotoxin (< 0.05 EU/mL release specification) and mycoplasma-retentive filtration for sensitive iPSC protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled media for HUVEC monolayer integrity and TEER monitoring.
Metabolic Flux Analysis
Defined formulation provides a clean background for 13C isotope tracing and NMR-based metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate baseline supports confocal microscopy and biosensor platforms; riboflavin in this formulation contributes native autofluorescence typical of DMEM-based media.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (Low, 1 g/L) | [-] Sodium Pyruvate, [-] Phenol Red |
| Appearance | Pale yellow to colorless, clear solution (phenol red-free) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310 – 350 mOsm/kg H2O |
| Glucose | 1000 mg/L (Low Glucose, 5.55 mM) |
| L-Glutamine | 584 mg/L (4.0 mM) |
| Sodium Pyruvate | Not added |
| Phenol Red | Not added |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| Total ingredients | 32 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Risk-mitigated via 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | ~10% CO2 (derived from 3700 mg/L NaHCO3 buffering at pH 7.4); supplemental 25 mM HEPES extends buffering stability for open-bench handling |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
32 ingredients verified per lot with CAS numbers for full raw-material traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity, produced under tight trace-metal and total organic carbon (TOC) control for consistent, reproducible culture performance.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every batch.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay, release specification: < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 310–350 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every batch on request.
How DCP-DMEMLH-PR1X compares
FluxMPS™ DCP-DMEMLH-PR1X vs. conventional 0.22 µm–filtered DMEM Low Glucose + HEPES formulations.
| Parameter | DCP-DMEMLH-PR1X (FluxMPS™) | Conventional DMEM Low Glucose + HEPES (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| HEPES-buffered — supplemental buffering alongside sodium bicarbonate, no Pyruvate/Phenol Red for imaging-clean carbon control | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMLH-PR1X.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered, microfluidics-suitable media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
