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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEMLH-QR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM Low Glucose + 25 mM HEPES formulation, engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. The formulation omits L-glutamine and phenol red for fresh-supplementation and imaging-clean workflows, and combines 44 mM sodium bicarbonate with 25 mM HEPES for buffering flexibility across incubator and open-bench handling. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Formulated with low glucose (1.0 g/L; 5.55 mM), 110 mg/L sodium pyruvate, and 3700 mg/L sodium bicarbonate, plus 25 mM HEPES (pKa 7.3 at 37°C) for dual-buffer flexibility
- Manufactured without L-glutamine (add fresh at time of use) and without phenol red, for imaging-clean, autofluorescence-conscious workflows
- Quadruple-stage filtration train — 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm — reaching a 0.04 µm final cut-off validated for microfluidic channel geometries
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Sterility confirmed by 14-day USP <71> incubation with no growth
- Manufactured under an ISO 13485:2016 quality management system; final QC and release at Diagnocine, Totowa, NJ
- Custom pH, glucose, salt, and buffer adjustments available on request — contact support@diagnocine.com
- Formulation[+] Sodium Bicarbonate, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Low Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red
- AppearancePale yellow, clear solution (phenol red-free)
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Total ingredients32
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma, subvisible particulates, and endotoxin fragments that clog microfluidic channels and corrupt sensor signals. FluxMPS™ targets these failure modes with four-stage sub-0.04 µm filtration. The dual bicarbonate/HEPES buffer system supports both closed-incubator and open-top chip handling.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion across chip geometries.
Total metabolic control
Selective inclusion/exclusion of glutamine, pyruvate, bicarbonate, and HEPES for precise nutrient and buffer definition. Cross-check the composition table before supplementing.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) minimizes trace-metal and total organic carbon (TOC) background introduced during formulation.
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy and biosensor applications requiring minimal scatter background.
Rich, stable nutrient profile
32 ingredients verified per lot; micro-batch production with full raw-material traceability.
Customization on demand
pH, glucose, salts, HEPES, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish, delivering purity levels not achieved by conventional single-pass 0.22 µm filtered media.
-
1
0.1 µm Prefiltration I
Removes large aggregates and particulates; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-DMEMLH-QR1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMLH-QR1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. The dual bicarbonate/HEPES buffer system suits both closed-incubator and open-top microfluidic devices.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers — the six-stage ultra nano-filtered line described in the Purity Architecture section above.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
- Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
0.04 µm-filtered media reduces microchannel clogging risk in complex multi-organ chip architectures.
CHO & Mammalian Cell Culture
Suited to CHO, cancer cell lines, primary cells, and clonal growth protocols requiring defined bicarbonate/HEPES buffering.
iPSC-Derived Models
Endotoxin release specification < 0.05 EU/mL and 0.1 µm mycoplasma-retentive filtration support sensitive iPSC protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled media for HUVEC monolayer integrity and TEER monitoring.
Metabolic Flux Analysis
Defined, glutamine-free formulation supports ¹³C isotope tracing and NMR metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Low particulate baseline supports confocal and biosensor platforms; note that riboflavin (a naturally fluorescent vitamin) remains part of this formulation.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Sodium Bicarbonate, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Low Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red |
| Appearance | Pale yellow, clear solution (phenol red-free) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H2O |
| Glucose | Low Glucose, 1000 mg/L (5.55 mM) |
| L-Glutamine | None / Not added |
| Sodium Pyruvate | 110 mg/L |
| Phenol Red | None / Not added |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| Total ingredients | 32 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1 (ASTM D1193 / ISO 3696), 18.2 MΩ·cm, low trace-metal/TOC |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | ~10% CO2 recommended for this 44 mM (3700 mg/L) NaHCO3 formulation to reach pH 7.4 (Henderson-Hasselbalch); 25 mM HEPES provides supplemental buffering during bench-top handling |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
32 ingredients verified per lot with CAS numbers for full raw-material traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 1000.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016–certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity, low trace-metal and TOC background — controls formulation-borne ionic and organic contaminants.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 310–350 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMLH-QR1X compares
FluxMPS™ DCP-DMEMLH-QR1X vs. conventional 0.22 µm–filtered DMEM Low Glucose + HEPES formulations.
| Parameter | DCP-DMEMLH-QR1X (FluxMPS™) | Conventional DMEM Low Glucose + HEPES (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not applicable | Not applicable |
| HEPES-buffered, phenol red-free formulation for imaging-clean workflows | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMLH-QR1X.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
