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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Phenol Red: 1X Liquid

Product#: DCP-DMEMH-R1X
$71.50
DCP-DMEMH-R1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Phenol Red: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Phenol Red

FluxMPS™ DCP-DMEMH-R1X is a Microfluidics Suitable, ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and CO2-independent or open-top microfluidic platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it reaches a 0.04 µm final polish for microchannel-safe purity. HEPES (25 mM, pKa 7.3 at 37°C) provides robust pH buffering independent of CO2 tension.

  • High Glucose (4.5 g/L) — supports energy-demanding cell types: neurons, cardiomyocytes, and cancer lines in long-duration perfusion
  • 25 mM HEPES (pKa 7.3 at 37°C) — robust pH buffering independent of CO2 tension, ideal for open-top and CO2-free platforms
  • Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II (Polish)
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Formulated without phenol red for reduced background absorbance in optical and biosensor assays
  • Manufactured under an ISO 13485:2016 quality management system with full lot traceability
  • 33 ingredients verified per lot across 4 composition categories; 4× BME amino acid/vitamin concentrations
  • Custom pH, glucose, HEPES concentration, salts, and nutrient adjustments available on request
CAT. NO.
DCP-DMEMH-R1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Phenol Red: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate, [-] Phenol Red
  • AppearancePale yellow to colorless, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)355–395 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized particles, subvisible particulates, and fine debris that can accumulate in microfluidic channels. Standard DMEM also lacks HEPES buffering, causing pH instability whenever chips are handled outside incubators. FluxMPS™ addresses both failure modes simultaneously.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance supports safe perfusion in sub-100 µm chip geometries.

bolt

High-energy cell support

4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.

water_drop

HEPES: CO2-stable pH

25 mM HEPES (pKa 7.3 at 37°C) maintains stable pH regardless of CO2 fluctuation — useful for open-top chips, point-of-care devices, and atmospheric incubation.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy and biosensor platforms on chip; this formulation contains no phenol red.

science

Rich, stable nutrient profile

33 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.

tune

Customization on demand

pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates. The 0.1 µm pore size provides mycoplasma-retentive filtration (mycoplasma range 0.2–0.3 µm), protecting the downstream 0.04 µm final filter.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated 0.1 µm mycoplasma-retentive prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 conditions.

Performance vs. conventional media

FluxMPS™ DCP-DMEMH-R1X is processed through two paired prefilter + final-filter stages, giving full redundancy and a 0.04 µm final pore size — well below the 0.22 µm standard used for conventional cell culture media.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final pore size
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved via 0.1 µm mycoplasma-retentive filtration at two stages of the train (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS(TM) DCP-DMEMH-R1X Dulbecco's Modified Eagle Medium (DMEM), High Glucose and 25mM HEPES w/o Phenol Red: 1X Liquid -- Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish -- Microfluidics Suitable DMEM High Glucose HEPES for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish.
© Diagnocine® — DCP-DMEMH-R1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEMH-R1X combines high-glucose energy support with HEPES pH stability — supporting demanding platforms from open-top microfluidic chips and CO2-free bioreactors to multi-organ body-on-a-chip systems.

Automated Bioreactors & Robotics

Next-Generation System Uptime

HEPES buffering reduces CO2 dependency during automated bioreactor perfusion. An optional 0.01 µm (10 nm) MPS Grade variant — the six-stage ultra nano-filtered line — is available on request for robotic liquid handlers where even trace particulates cause valve failure.

  • Reduced Particulate Load: The quadruple-stage 0.04 µm train removes fine particulate from bioreactor media lines
  • pH-Stable Automated Delivery: HEPES helps maintain pH during robotic media exchanges without immediate CO2 re-equilibration
  • Extended Perfusion Stability: Consistent high-glucose delivery with buffered pH over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Open-Top & CO2-Free Chips

HEPES buffering enables pH-stable culture in open-top microfluidic devices, atmospheric incubators, and multi-compartment chips with heterogeneous CO2 environments.

OoCToCBoCLoCMPS
Neuroscience

Primary Neurons & Brain-on-Chip

High glucose (4.5 g/L) plus HEPES stability supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion.

iPSC-NeuronsPrimary neuronsBrain-on-chip
Cardiac Biology

Cardiomyocyte & Heart-on-Chip

High-glucose DMEM plus HEPES is a common base for iPSC-CM maturation and heart-on-chip functional assays requiring buffered pH and high energy substrate.

iPSC-CMHeart-on-chipTEER
Cancer Biology

Warburg Effect & Cancer Models

High glucose supports aerobic glycolysis in cancer lines; HEPES helps stabilize pH during rapid glucose consumption in Warburg-active tumour models.

MCF-7MDA-MB-231HeLaA549
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation for ¹³C isotope tracing and NMR metabolomics; HEPES allows sampling outside incubators without immediate pH artefacts. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

HEPES-stabilized pH during imaging sessions; this phenol red–free formulation reduces background absorbance for confocal and biosensor platforms.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate, [-] Phenol Red
Appearance Pale yellow to colorless, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 355–395 mOsm/kg H2O
Total ingredients 33 across 4 categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement HEPES-buffered (25 mM); CO2-independent operation supported. Compatible with standard 5% CO2 incubation via sodium bicarbonate co-buffering.
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 33 ingredients verified per lot across 4 categories with CAS numbers for raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the OTHERS group.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 4500.000
Sodium pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-DMEMH-R1X custom specifications — pH, glucose, HEPES concentration, salts, or nutrient modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA and testing at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity with low trace-metal and organic-carbon (TOC) content, minimizing extraneous contaminant load in the finished medium.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.

assignment

Micro-Batch Precision

Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.

Endotoxin — USP <85> BET

LAL assay; batch release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 355–395 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-R1X compares

FluxMPS™ DCP-DMEMH-R1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEMH-R1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard DMEM HG + HEPES
(0.22 µm filtered)
Grade Microfluidics Suitable Not applicable Not applicable
HEPES-buffered High Glucose DMEM, phenol red–free formulation (reduced background absorbance, no estrogen-agonist interference) check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes — 0.1 µm retentive filtration cancel No cancel No
HEPES buffer (25 mM) check_circle Yes cancel No check_circle Yes
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-R1X DMEM High Glucose + 25mM HEPES.

Yes. DCP-DMEMH-R1X combines Quadruple-stage 0.04 µm filtration for reduced particulate delivery in microfluidic channels with 25 mM HEPES buffering for CO2-tolerant pH. High glucose (4.5 g/L) supports energy-demanding cell types — neurons, cardiomyocytes, and cancer lines — in long-duration chip perfusion. The HEPES component is particularly useful for open-top chips where CO2 concentration fluctuates.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish) — reaching a 0.04 µm final pore size, well below the 0.22 µm standard used for conventional media, with 0.1 µm mycoplasma-retentive filtration at each prefiltration stage.
Phenol red is a weak estrogen-receptor agonist at µM concentrations and can contribute background absorbance/fluorescence around 520–560 nm. Removing it reduces optical background for confocal microscopy, TEER sensors, and biosensor arrays on chip (residual background from riboflavin remains), while HEPES maintains pH stability independent of CO2. This formulation already contains glucose, sodium pyruvate, L-glutamine, and sodium bicarbonate; no further carbon-source or buffer supplementation is required for most standard applications. Contact support@diagnocine.com for cell-type-specific guidance.
HEPES-buffered (25 mM); CO2-independent operation is supported. This formulation is also compatible with standard 5% CO2 incubation via its sodium bicarbonate co-buffer, making it suitable for both open-top chips and conventional incubators.
Yes. Add FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or custom nutrients as required. When filtering serum-containing additions, use a 0.2 µm low-protein-binding PES or PVDF filter — a 0.04 µm membrane will strip serum of proteins, lipoproteins, and growth factors and should never be used for supplement filtration. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by LAL assay (USP <85>) before release and must meet the release specification of < 0.05 EU/mL. A Certificate of Analysis reporting the lot-specific result is available on request at support@diagnocine.com.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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