FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o Sodium Bicarbonate: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Sodium bicarbonate is not added, so buffering is user-defined for CO₂-independent or custom-buffered culture.

• Quadruple-stage filtration to a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2), sterile-processed for microfluidic and organ-on-a-chip perfusion
• Formulated with 3.8255 g/L D-glucose, L-glutamine (474.5 mg/L) and sodium pyruvate (27.525 mg/L), but without sodium bicarbonate — add bicarbonate matched to your CO₂ level or 10–25 mM HEPES for ambient-air culture
• Endotoxin release specification < 0.05 EU/mL (USP <85> BET), tested per manufacturing batch
• Manufactured under an ISO 13485:2016 quality management system; final packaging and QC at DiagnoCine, Totowa, NJ
• Contains phenol red (12.235 mg/L) as a pH indicator — not compatible with phenol-red-free assay platforms such as Agilent Seahorse XF
• 4× BME amino acid and vitamin concentrations supporting extended microphysiological system (MPS) perfusion
• Custom pH, glucose, HEPES, salts & nutrient formulations available — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and complicating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

FluxMPS™ DMEM/F-12 (3:1) w/o Sodium Bicarbonate undergoes a validated four-stage sequential filtration reaching a final pore size of 0.04 µm — capturing sub-micron particulates and mycoplasma-sized organisms that pass a standard 0.22 µm filter.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter cartridge.

2. 2

   0.04 µm Final filtration I

   Retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including mycoplasma-sized organisms (0.2–0.3 µm).

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o Sodium Bicarbonate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

For automated bioreactor and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available — the six-stage cascade beyond this Microfluidics Suitable product.

• Total particulate exclusion beyond the 0.04 µm Microfluidics Suitable tier
• Valve & sensor protection for long-duration automated perfusion systems
• Extended perfusion stability for unattended bioreactor runs

Inquiry Required: the 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request specifications and lead time.

Full technical specification

Every lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o Sodium Bicarbonate: 1X Liquid is released against multi-parameter QC specifications.

Full composition (mg/L)

Total: 50 components across 4 categories — Inorganic Salts, Amino Acids, Vitamins, and Others (grouped into 3 tabs below). All values are per-lot verified and reported on the Certificate of Analysis (CoA). 4× BME amino acid and vitamin concentrations.

Manufacturing & compliance

Every batch is subjected to multi-parameter release testing before distribution.

How DCP-DMF12-BT1X compares

Filtration, mycoplasma-retentive filtration, water quality, and QC depth differences vs conventional media for MPS and microfluidic applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o Sodium Bicarbonate: 1X Liquid (DCP-DMF12-BT1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x