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- FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o L-Glutamine: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o L-Glutamine: 1X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) w/o L-Glutamine: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/Ham’s F-12 (3:1) cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 using Ultrapure Type 1 water (18.2 MΩ·cm). Formulated without L-glutamine for user-defined nitrogen-source control, with a dual sodium bicarbonate/HEPES buffering system for extended pH stability.
- Quadruple-stage filtration to a 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2) — mycoplasma-retentive at every stage
- Endotoxin release specification: < 0.05 EU/mL (USP <85> BET), tested per manufacturing batch
- DMEM/Ham’s F-12 (3:1) base with 3825.5 mg/L D-glucose, 27.525 mg/L sodium pyruvate, and dual sodium bicarbonate/HEPES buffering (3070 mg/L NaHCO₃ + 15 mM HEPES)
- Formulated without L-glutamine — add stabilized L-glutamine or a dipeptide substitute (e.g., GlutaMAX) at time of use
- Contains phenol red (12.235 mg/L) as a visual pH indicator
- Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon (TOC) control
- Custom pH, glucose, HEPES, salts & nutrients available — support@diagnocine.com
- Glucose3825.500 mg/L (Present)
- L-GlutamineRemoved ([-])
- Sodium Pyruvate27.525 mg/L ([+])
- CO₂ Requirement~8% (derived from 3070 mg/L NaHCO₃)
- Osmolality (USP <785>)320 - 360 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.
Microchannel-safe purity
0.04 µm final filtration reduces particulate load below what conventional 0.22 µm filtration captures, supporting unobstructed flow across typical microfluidic channel geometries.
Total metabolic control
Precisely defined carbon and nitrogen sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for tight trace-metal and organic-carbon (TOC) control across every batch.
Low background for imaging
Reduced particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
Rich amino acid and vitamin profile from the DMEM/Ham’s F-12 (3:1) blend, produced with micro-batch precision for tight lot-to-lot consistency in long-duration MPS perfusion.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ DCP-DMF12-QT1X undergoes a validated four-stage sequential filtration reaching a final pore size of 0.04 µm — engineered to capture particulates and bioburden that pass through conventional 0.22 µm filtration.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final-filter cartridge.
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2
0.04 µm Final filtration I
Retains fine particulates and mycoplasma-sized organisms (0.2–0.3 µm) entirely absent from standard 0.22 µm media.
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3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge for full redundancy.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill in an ISO Class 5 (Class 100) laminar-flow workstation.
Performance vs. conventional media
The quadruple-stage 0.1 µm / 0.04 µm train removes particulates and bioburden that a single-pass 0.22 µm filtration cannot capture, supporting channel integrity across long-duration microfluidic perfusion.
© Diagnocine® — DCP-DMF12-QT1X
Optimized for next-generation cell biology platforms
FluxMPS™ DCP-DMF12-QT1X is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.
Automated Bioreactors & Robotics
For automated bioreactors and robotic liquid-handling platforms, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation — a separate six-stage process beyond the Microfluidics Suitable 0.04 µm line described on this page.
- Total particulate exclusion for closed automated perfusion loops
- Valve & sensor protection in robotic liquid-handling systems
- Extended perfusion stability for multi-day unattended runs
Inquiry Required: The 0.01 µm MPS Grade variant is available on request. Contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultra-low particulate, mycoplasma-retentive media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Defined glucose and pyruvate content supports Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-reduced perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Reduced particulate baseline for confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Full technical specification
Every lot of FluxMPS™ DCP-DMF12-QT1X is released against comprehensive multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] Sodium Bicarbonate, Phenol Red, HEPES, Calcium, Magnesium, Glucose, Sodium Pyruvate / [-] L-Glutamine |
| Appearance | Clear, red-pink solution (phenol red indicator) |
| Osmolality USP <785> | 320 - 360 mOsm/kg H₂O |
| Glucose | 3825.500 mg/L (Present) |
| L-Glutamine | Removed ([-]) |
| Sodium Pyruvate | 27.525 mg/L ([+]) |
| Phenol Red | 12.235 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL (batch release) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO₂ requirement | ~8% CO₂ recommended (derived from 3070 mg/L NaHCO₃; validate empirically per incubator/application) |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
| Pack Sizes | 500 mL, 1000 mL |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 51 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| Calcium chloride dihydrate | 10035-04-8 | 209.775 |
| Copper sulfate pentahydrate | 7758-99-8 | 0.000625 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 35.510 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.075 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.209 |
| Magnesium chloride hexahydrate | 7791-18-6 | 14.412 |
| Magnesium sulfate anhydrous | 7487-88-9 | 73.290 |
| Potassium chloride | 7447-40-7 | 356.000 |
| Sodium bicarbonate | 144-55-8 | 3070.000 |
| Sodium chloride | 7647-14-5 | 6699.750 |
| Sodium dihydrogen phosphate monohydrate | 10049-21-5 | 81.750 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.215 |
| Component | CAS Number | mg/L |
|---|---|---|
| Glycine | 56-40-6 | 24.380 |
| L-Alanine | 56-41-7 | 2.227 |
| L-Arginine hydrochloride | 1119-34-2 | 115.520 |
| L-Asparagine monohydrate | 5794-13-8 | 3.752 |
| L-Aspartic acid | 56-84-8 | 3.325 |
| L-Cysteine dihydrochloride | 8.780 | |
| L-Cystine hydrochloride monohydrate | 46.927 | |
| L-Glutamic acid | 56-86-0 | 3.675 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 36.740 |
| L-Isoleucine | 73-32-5 | 79.735 |
| L-Leucine | 61-90-5 | 82.025 |
| L-Lysine hydrochloride | 657-27-2 | 118.625 |
| L-Methionine | 63-68-3 | 23.625 |
| L-Phenylalanine | 63-91-2 | 50.740 |
| L-Proline | 147-85-3 | 8.625 |
| L-Serine | 56-45-1 | 34.125 |
| L-Threonine | 72-19-5 | 74.225 |
| L-Tryptophan | 73-22-3 | 12.510 |
| L-Tyrosine disodium salt | 79.795 | |
| L-Valine | 72-18-4 | 73.425 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 6.490 |
| D-Biotin | 58-85-5 | 0.001825 |
| D-Ca-Pantothenate | 137-08-6 | 3.120 |
| Folic acid | 59-30-3 | 3.330 |
| Nicotinamide | 98-92-0 | 3.000 |
| Pyridoxine hydrochloride | 58-56-0 | 3.015 |
| Riboflavin | 83-88-5 | 0.309 |
| Thiamine hydrochloride | 67-03-8 | 3.085 |
| Vitamin B12 | 68-19-9 | 0.340 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 3825.500 |
| DL-Thioctic acid | 1077-28-7 | 0.053 |
| HEPES buffer | 7365-45-9 | 3574.500 |
| Hypoxanthine | 68-94-0 | 1.200 |
| Linoleic acid | 60-33-3 | 0.021 |
| myo-Inositol | 87-89-8 | 9.900 |
| Phenol red sodium salt | 34487-61-1 | 12.235 |
| Putrescine hydrochloride | 0.040 | |
| Sodium pyruvate | 113-24-6 | 27.525 |
| Thymidine | 50-89-5 | 0.183 |
Manufacturing & compliance
Every batch subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, produced with tight trace-metal and organic-carbon (TOC) control for every batch.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves filtration gains in the final container.
Micro-Batch Precision
Small-batch production for tight lot-to-lot consistency in osmolality, endotoxin, and other release parameters — critical for reproducible long-duration MPS experiments.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay. Batch release specification: < 0.05 EU/mL. Assay sensitivity 0.005 EU/mL.
Sterility — USP <71>
No growth after 14-day incubation, tested per lot.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 320 - 360 mOsm/kg H₂O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
How DCP-DMF12-QT1X compares
Critical advantages in filtration, mycoplasma-retentive processing, water quality, and QC depth for microfluidic and MPS applications.
| Parameter | DCP-DMF12-QT1X (FluxMPS™) | Standard DMEM/F-12 (0.22 µm) | Competitor DMEM/F-12 (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not applicable | Not applicable |
| Formulation | [+] Sodium Bicarbonate, Phenol Red, HEPES, Calcium, Magnesium, Glucose, Sodium Pyruvate / [-] L-Glutamine | Standard formulation | Standard formulation |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate compliance | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMF12-QT1X.
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
