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FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid

Product#: DCP-DMF12H-QBCT1X
$52.80
DCP-DMF12H-QBCT1X
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verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid

[+] 3.8255 g/L Glucose[+] Sodium Pyruvate[+] Sodium Bicarbonate[+] 15mM HEPES [-] L-Glutamine[-] Sodium Bicarbonate[-] Calcium Chloride

MPS-grade, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Approximately 5× lower particulate count than conventional 0.22 µm-filtered media.

  • Quadruple-stage 0.04 µm final filtration — mycoplasma-free, sub-micron particle–free
  • Endotoxin: NMT 1EU/ml (USP <85> BET lot release)
  • Ultrapure Type 1 water (18.2 MΩ·cm) — zero ionic background for biosensor chips
  • USP <788> Method 2 particulate compliance — microchannel-safe down to 50 µm
  • ISO Class 5 (Class 100) laminar-flow aseptic fill & finish
  • 4× BME amino acid & vitamin concentrations for robust MPS perfusion
  • Custom pH, glucose, HEPES, salts & nutrients — support@diagnocine.com
DCP-DMF12H-QBCT1X · UNSPSC 41122100 · Cell Culture Media
Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid 
  • GlucosePresent
  • L-GlutamineRemoved ([-])
  • Sodium Pyruvate27.525 mg/L ([+])
  • pH (USP <791>)1
  • Osmolality (USP <785>)260.00 - 300.00 mOsm/kg
  • Endotoxin (USP <85>)NMT 1EU/ml
  • Filtration0.1µm ×2 + 0.04µm ×2
  • Storage2-8°C, away from bright light
  • Shelf Life2-8°C, away from bright light
  • ShippingCold pack (2–8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.1–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

filter_alt

Microchannel-safe purity

0.04 µm final filter and USP <788> particulate compliance ensure unobstructed flow in channels as narrow as 50 µm.

target

Total metabolic control

Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing without background noise.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm, USP <85>) — eliminating ionic interference in TEER and electrochemical biosensors.

visibility

Low background for imaging

Ultra-low particulate baseline for pristine confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.

science

Rich, stable nutrient profile

4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

The only ready-to-use cell culture medium with a validated four-stage sequential filtration reaching a final pore size of 0.04 µm — capturing what 0.22 µm filtration misses entirely.

  1. 1

    0.1 µm Pre-filtration I

    Removes large particulates, cell debris, and protein aggregates. Protects downstream fine filters and preserves microchannel chip geometries.

  2. 2

    0.04 µm Pre-filtration II

    Retains fine particulates, bacteria, and mycoplasma (typical diameter 0.1–0.3 µm) — entirely absent from standard 0.22 µm media.

  3. 3

    0.1 µm Sterile-filtration I

    Second-pass redundant sterile filtration for validated double bioburden clearance.

  4. 4

    0.04 µm Sterile-filtration II — Final Polish

    Ultimate polishing filter; aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstation.

Performance vs. conventional media

By USP <788> Method 2, FluxMPS™ delivers ~5× fewer particles ≥10 µm vs standard 0.22 µm filtered media — the difference between successful long-duration perfusion and channel occlusion within 48 h.

5×
Fewer particles ≥10 µm vs 0.22 µm media
0.04
µm Final filter — sub-mycoplasma polishing
Sterility & Mycoplasma: 14-day USP <71> sterility tested per lot. Dual 0.04 µm filtration provides USP <63>-equivalent mycoplasma barrier. Smallest known mycoplasma ≈ 0.2 µm — well above the 0.04 µm cut-off.
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid (DCP-DMF12H-QBCT1X) ? Quadruple-stage filtration system (0.1 μm ×2 + 0.04 μm ×2) for organ-on-a-chip and microfluidic MPS cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Pre-I → 0.04µm Pre-II → 0.1µm Sterile-I → 0.04µm Sterile-II (Final Polish) → ISO Class 5 aseptic fill.
© Diagnocine® — DCP-DMF12H-QBCT1X
Formulation

Full Composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 49 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

INORGANIC SALTS

Component CAS Number mg/L
Copper sulphate pentahydrate 7758-99-8 0.000625
Disodium hydrogen phosphate anhydrous 7558-79-4 35.510
Ferric nitrate nonahydrate 7782-61-8 0.075
Ferrous sulphate heptahydrate 7782-63-0 0.209
Magnesium chloride hexahydrate   14.412
Magnesium sulphate anhydrous 7487-88-9 73.290
Potassium chloride 7447-40-7 356.000
Sodium chloride 7647-14-5 6699.750
Sodium dihydrogen phosphate monohydrate   81.750
Zinc sulphate heptahydrate 7446-20-0 0.215

AMINO ACIDS

Component CAS Number mg/L
Glycine 56-40-6 24.380
L-Alanine 56-41-7 2.227
L-Arginine hydrochloride 1119-34-2 115.520
L-Asparagine monohydrate 5794-13-8 3.752
L-Aspartic acid 56-84-8 3.325
L-Cysteine dihydrochloride   8.780
L-Cystine hydrochloride monohydrate   46.927
L-Glutamic acid 56-86-0 3.675
L-Histidine hydrochloride monohydrate 5934-29-2 36.740
L-Isoleucine 73-32-5 79.735
L-Leucine 61-90-5 82.025
L-Lysine hydrochloride 657-27-2 118.625
L-Methionine 63-68-3 23.625
L-Phenylalanine 63-91-2 50.740
L-Proline 147-85-3 8.625
L-Serine 56-45-1 34.125
L-Threonine 72-19-5 74.225
L-Tryptophan 73-22-3 12.510
L-Tyrosine disodium salt   79.795
L-Valine 72-18-4 73.425
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 6.490
D-Biotin   0.001825
D-Ca-Pantothenate 137-08-6 3.120
Folic acid 59-30-3 3.330
Nicotinamide 98-92-0 3.000
Pyridoxine hydrochloride 58-56-0 3.015
Riboflavin 83-88-5 0.309
Thiamine hydrochloride 67-03-8 3.085
Vitamin B12 68-19-9 0.340
myo-Inositol   9.900
OTHERS
D-Glucose 50-99-7 3825.500
DL-Thioctic acid   0.053
HEPES buffer   3574.500
Hypoxanthine 68-94-0 1.200
Linoleic acid 60-33-3 0.021
Phenol red sodium salt 34487-61-1 12.235
Putrescine hydrochloride   0.040
Sodium pyruvate 113-24-6 27.525
Thymidine 50-89-5 0.183
Custom formulations: pH, glucose, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Technical Specifications

Full technical specification

Every lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 3.8255 g/L Glucose, Sodium Pyruvate, Sodium Bicarbonate, 15mM HEPES / [-] L-Glutamine, Sodium Bicarbonate, Calcium Chloride
Appearance Clear solution
pH USP <791> 1
Osmolality USP <785> 260.00 - 300.00 mOsm/kg H₂O
Glucose Present
L-Glutamine Removed ([-])
Sodium Pyruvate 110 mg/L ([+])
Phenol Red 15.9 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> NMT 1EU/ml
Sterility USP <71> No growth after 14 days
Mycoplasma USP <63> Prevented — dual 0.04 µm
Particulate ≥10 µm <788> ~5× below 0.22 µm media
Particulate ≥25 µm <788> Compliant
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage & Logistics
Parameter Specification
Storage 2-8°C, away from bright light
Freeze–thaw Do not freeze
Shelf life 2-8°C, away from bright light
Shipping Cold pack (2–8°C)
CO₂ requirement 5% CO₂ incubator required
Regulatory & Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Manufacturing QMS ISO 13485:2016 ISO
Regulatory 21 CFR Part 820 aligned; CE-approved
Production Micro-batch; Totowa, NJ, USA
Intended use RUO only
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Microfluidics

Organ-on-a-Chip (OoC) & MPS

Ultra-low particulate, mycoplasma-free media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.

OoCToCBoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Glucose-controlled or glucose-free formulations for Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-free perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined formulations for ¹³C metabolic tracing, Seahorse XF respiratory assays, and NMR metabolomics.

¹³C tracingSeahorse XFNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Optically clear, low-autofluorescence medium for confocal microscopy, fluorescent biosensors, and automated imaging on chip.

ConfocalBiosensorsTEER
Quality Assurance

Manufacturing & compliance

Every batch subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified, CE-approved suppliers. Final packaging, QA and testing at DiagnoCine R&D Center; customization at DiagnoCine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, <0.001 ppm TOC, USP <85> endotoxin-tested. Eliminates ionic background that corrupts biosensor chips and TEER systems.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves all filtration gains in the final container.

assignment

Micro-Batch Precision

Small-batch production: osmolality (±5 mOsm/kg), pH (±0.05), endotoxin tight — critical for reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL kinetic turbidimetric method. Lot release: NMT 1EU/ml. FluxMPS target: <0.05 EU/mL.

Particulate — USP <788> Method 2

~5× lower particles ≥10 µm vs 0.22 µm filtered media by light-obscuration count.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 260.00 - 300.00 mOsm/kg H₂O.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

CoA Request: Available at no charge for any production lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid (DCP-DMF12H-QBCT1X). Email support@diagnocine.com.
Product Comparison

How DCP-DMF12H-QBCT1X compares

Critical advantages in filtration, mycoplasma barrier, water quality, and QC depth that conventional media cannot match for MPS and microfluidic applications.

Parameter DCP-DMF12H-QBCT1X (FluxMPS™) Standard DMEM/F-12 (0.22 µm) Competitor DMEM/F-12 (0.22 µm)
Formulation definition [+] 3.8255 g/L Glucose, Sodium Pyruvate, Sodium Bicarbonate, 15mM HEPES / [-] L-Glutamine, Sodium Bicarbonate, Calcium Chloride Standard Standard
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier check_circle cancel cancel
Endotoxin spec NMT 1EU/ml Typically <1 EU/mL Varies
USP <788> particulate check_circle ~5× lower cancel cancel
Water quality Ultrapure Type 1, 18.2 MΩ·cm Unspecified Unspecified
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel validated check_circle cancel cancel
Custom formulation check_circle On request cancel cancel
FAQ

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid (DCP-DMF12H-QBCT1X).

DCP-DMF12H-QBCT1X is specifically engineered for organ-on-a-chip, microfluidic, and MPS applications. The 0.04 µm final filtration removes mycoplasma and sub-micron particulates that cause microchannel clogging in standard 0.22 µm media.
Four sequential stages (0.1 µm ×2 + 0.04 µm ×2) remove mycoplasma (0.1–0.3 µm diameter), sub-visible particulates, and bioburden entirely missed by conventional 0.22 µm filters — resulting in ~5× fewer particles by USP <788> Method 2.
DCP-DMF12H-QBCT1X lacks sodium bicarbonate to allow CO₂-independent incubation or custom buffering. Add 10–25 mM HEPES for ambient-air culture, or supplement bicarbonate matched to your CO₂ level. Contact support@diagnocine.com for advice.
Yes — DCP-DMF12H-QBCT1X contains sodium bicarbonate and requires 5% CO₂/95% air incubator for physiological pH 7.4. For CO₂-independent use, add 10–25 mM HEPES or request the bicarbonate-free variant.
DCP-DMF12H-QBCT1X is a basal medium fully compatible with FBS (2–10%), human serum, GlutaMAX, EGF, FGF, VEGF, and antibiotics. Pre-filter all additions through a 0.04 µm or 0.1 µm syringe filter before combining to maintain MPS-grade purity.
Lot release: NMT 1EU/ml by USP <85> LAL kinetic turbidimetric method. FluxMPS™ target: <0.05 EU/mL. Lot-specific endotoxin value on CoA — request at support@diagnocine.com.
Yes — full CoA per lot includes: lot number, expiry, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), mycoplasma clearance, particulate count (USP <788>), and cultural response. Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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