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FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid

Product#: DCP-DMF12H-QBCT1X
$52.80
DCP-DMF12H-QBCT1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid

Contains Phenol Red Contains 15mM HEPES Contains Magnesium Contains 3.8255 g/L Glucose Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate Without Calcium Chloride

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/F-12 (3:1) medium buffered with 15 mM HEPES, engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 using Ultrapure Type 1 water (18.2 MΩ·cm). This formulation omits L-glutamine, sodium bicarbonate, and calcium chloride, giving you full control over supplementation and buffering strategy. Approximately 5× fewer particles ≥10 µm than conventional 0.22 µm-filtered media (USP <788> Method 1).

  • Quadruple-stage filtration to a 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2); Sterile, USP <71> tested per lot
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • DMEM/F-12 (3:1) base with 15 mM HEPES buffering; formulated without L-glutamine, sodium bicarbonate, or calcium chloride for custom supplementation
  • Glucose 3.8255 g/L and sodium pyruvate 27.525 mg/L included as standard carbon-source components
  • Contains phenol red (12.235 mg/L) as a pH indicator — a phenol-red-free variant is available on request
  • Manufactured under an ISO 13485:2016 quality management system; micro-batch production finished at Diagnocine, Totowa NJ
  • Prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
  • Custom pH, glucose, HEPES, salts & nutrients — support@diagnocine.com
DCP-DMF12H-QBCT1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride: 1X Liquid
  • Glucose3.8255 g/L ([+])
  • L-GlutamineNot added ([-])
  • Sodium Pyruvate27.525 mg/L ([+])
  • CO₂ RequirementHEPES-buffered; reduced CO₂ dependence
  • Osmolality (USP <785>)260 - 300 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2
  • Storage2-8°C, away from bright light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2–8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and confounding metabolic assays.[1,2] FluxMPS™ closes that gap with a validated multi-stage filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filter and USP <788> Method 1 (Light Obscuration) particulate testing support unobstructed flow through narrow microfluidic channels.

target

Total metabolic control

Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing.[3]

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic-carbon content, supporting consistent formulation lot to lot.

visibility

Low background for imaging

Ultra-low particulate baseline reduces debris interference in confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip. Note: this formulation contains phenol red, which may contribute assay background — a phenol-red-free variant is available on request.

science

Rich, stable nutrient profile

4× BME amino acid and vitamin concentrations with micro-batch production for tight lot-to-lot consistency across long-duration MPS perfusion.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaches a final pore size of 0.04 µm, using two dedicated prefilter + final-filter pairs run in series.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish.

Performance vs. conventional media

By USP <788> Method 1 (Light Obscuration), FluxMPS™ delivers approximately 5× fewer particles ≥10 µm vs standard 0.22 µm filtered media.

5×
Fewer particles ≥10 µm vs 0.22 µm media
0.04
µm final filter across 4 sequential passes
Sterility & Mycoplasma: 14-day USP <71> sterility tested per lot. Mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration (not tested per lot). Reference mycoplasma diameter: 0.2–0.3 µm.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DMEM/F-12 (3:1) with 15mM HEPES, w/o L-Glutamine, Sodium Bicarbonate, Calcium Chloride (DCP-DMF12H-QBCT1X) - Quadruple-stage filtration system (0.1 micron x2 + 0.04 micron x2) for organ-on-a-chip and microfluidic MPS cell culture | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → aseptic fill.
© Diagnocine® — DCP-DMF12H-QBCT1X
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ DCP-DMF12H-QBCT1X is validated for applications where microchannel cleanliness, signal fidelity, and buffering flexibility are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For long-duration automated perfusion and robotic bioreactor systems, an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available — the separate six-stage tier described in the Grade note above.

  • Total Particulate Exclusion — minimizes valve and sensor fouling in closed-loop systems
  • Valve & Sensor Protection — reduces micro-debris interference with optical and electrochemical sensors
  • Extended Perfusion Stability — supports multi-week unattended culture runs

Inquiry Required: the 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to discuss your automated system requirements.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low particulate media for perfusion in microfluidic chips, tissue chips, and multi-organ devices.

OoCToCBoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined glucose and pyruvate content supports Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.[3]

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Calcium-chloride-free, particle-managed perfusion medium suited to TEER measurement, endothelial monolayer integrity, and primary cell culture protocols where extracellular calcium must be user-controlled.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium — this formulation contains phenol red.

¹³C tracingNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate medium for confocal microscopy, fluorescent biosensors, and automated imaging on chip. Contains phenol red; a phenol-red-free variant is available for fluorescence-sensitive assays.

ConfocalBiosensorsTEER
Technical Specifications

Full technical specification

Every lot of FluxMPS™ DCP-DMF12H-QBCT1X is released against multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, 15mM HEPES, Magnesium, 3.8255 g/L Glucose, Sodium Pyruvate / [-] L-Glutamine, Sodium Bicarbonate, Calcium Chloride
Appearance Red-pink, clear liquid (phenol red present as pH indicator)
Osmolality USP <785> 260 - 300 mOsm/kg
Glucose 3.8255 g/L ([+])
L-Glutamine Not added ([-])
Sodium Pyruvate 27.525 mg/L ([+])
Phenol Red 12.235 mg/L
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release spec)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, away from bright light
Freeze–thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2–8°C)
CO₂ requirement HEPES-buffered; reduced CO₂ dependence (validate per cell line)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production Micro-batch; Totowa, NJ, USA
Intended use RUO only

Available pack sizes: 500 mL, 1000 mL.

Formulation

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 49 components across 4 ingredient categories (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 tabs below. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Component CAS Number mg/L
INORGANIC SALTS
Copper sulphate pentahydrate 7758-99-8 0.000625
Disodium hydrogen phosphate anhydrous 7558-79-4 35.510
Ferric nitrate nonahydrate 7782-61-8 0.075
Ferrous sulphate heptahydrate 7782-63-0 0.209
Magnesium chloride hexahydrate 7791-18-6 14.412
Magnesium sulphate anhydrous 7487-88-9 73.290
Potassium chloride 7447-40-7 356.000
Sodium chloride 7647-14-5 6699.750
Sodium dihydrogen phosphate monohydrate   81.750
Zinc sulphate heptahydrate 7446-20-0 0.215
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 24.380
L-Alanine 56-41-7 2.227
L-Arginine hydrochloride 1119-34-2 115.520
L-Asparagine monohydrate 5794-13-8 3.752
L-Aspartic acid 56-84-8 3.325
L-Cysteine dihydrochloride 7048-04-6 8.780
L-Cystine hydrochloride monohydrate   46.927
L-Glutamic acid 56-86-0 3.675
L-Histidine hydrochloride monohydrate 5934-29-2 36.740
L-Isoleucine 73-32-5 79.735
L-Leucine 61-90-5 82.025
L-Lysine hydrochloride 657-27-2 118.625
L-Methionine 63-68-3 23.625
L-Phenylalanine 63-91-2 50.740
L-Proline 147-85-3 8.625
L-Serine 56-45-1 34.125
L-Threonine 72-19-5 74.225
L-Tryptophan 73-22-3 12.510
L-Tyrosine disodium salt   79.795
L-Valine 72-18-4 73.425
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 6.490
D-Biotin 58-85-5 0.001825
D-Ca-Pantothenate 137-08-6 3.120
Folic acid 59-30-3 3.330
Nicotinamide 98-92-0 3.000
Pyridoxine hydrochloride 58-56-0 3.015
Riboflavin 83-88-5 0.309
Thiamine hydrochloride 67-03-8 3.085
Vitamin B12 68-19-9 0.340
myo-Inositol 87-89-6 9.900
OTHERS
D-Glucose 50-99-7 3825.500
DL-Thioctic acid 1200-22-2 0.053
HEPES buffer 7365-45-9 3574.500
Hypoxanthine 68-94-0 1.200
Linoleic acid 60-33-3 0.021
Phenol red sodium salt 34487-61-1 12.235
Putrescine hydrochloride 333-93-7 0.040
Sodium pyruvate 113-24-6 27.525
Thymidine 50-89-5 0.183
Custom formulations: pH, glucose, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, low TOC, used throughout formulation. Controlled trace-metal and organic-carbon content supports consistent lot-to-lot formulation.

biotech

Quadruple-Stage Filtration

0.1 µm ×2 + 0.04 µm ×2 sequential filtration prior to aseptic fill and finish.

assignment

Micro-Batch Precision

Small-batch production with per-lot osmolality, endotoxin, and sterility testing — supporting reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL assay. Release specification: < 0.05 EU/mL. Controlled per manufacturing batch, not per unit.

Particulate — USP <788> Method 1

Light Obscuration Particle Count Test. Approximately 5× fewer particles ≥10 µm vs 0.22 µm filtered media.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 260 - 300 mOsm/kg.

Documentation / CoA

Full Certificate of Analysis per lot. Request at support@diagnocine.com with lot number.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMF12H-QBCT1X compares

Key differences in grade, filtration, mycoplasma-risk mitigation, endotoxin specification, and QC depth versus conventional 0.22 µm filtered DMEM/F-12 media.

Parameter DCP-DMF12H-QBCT1X (FluxMPS™) Conventional DMEM/F-12 (0.22 µm) Standard alternative (0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
Buffering 15 mM HEPES, bicarbonate-free Sodium bicarbonate Sodium bicarbonate
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration (0.1 µm) check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate check_circle ~5× fewer particles ≥10 µm (Method 1) cancel cancel
Water quality Ultrapure Type 1, 18.2 MΩ·cm Unspecified Unspecified
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle On request cancel cancel

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMF12H-QBCT1X.

Yes. DCP-DMF12H-QBCT1X is engineered for organ-on-a-chip, microfluidic, and MPS applications, and is Microfluidics Suitable at a 0.04 µm final filtration cut-off. This filtration reduces particulates and organisms that can obstruct microchannel flow paths in standard 0.22 µm media.
Four sequential stages (0.1 µm ×2 + 0.04 µm ×2) target particulates and organisms that pass through conventional 0.22 µm filters, including mycoplasma-range organisms (0.2–0.3 µm), evaluated per USP <788> Method 1 (Light Obscuration).
L-Glutamine is omitted so it can be added fresh at time of use, or replaced with a stable dipeptide substitute such as GlutaMAX. Sodium Bicarbonate is omitted because this formulation is buffered instead with 15 mM HEPES for reduced CO₂ dependence; contact us if a bicarbonate-buffered variant is required. Calcium Chloride is omitted for protocols requiring calcium-free or user-defined calcium conditions — add calcium chloride to your required concentration if your cell type needs it.
No. This formulation is buffered with 15 mM HEPES and contains no sodium bicarbonate, so it does not require a 5% CO₂ atmosphere for pH stability. It is suited to ambient-air (CO₂-independent) incubation; actual requirements should be validated for your specific cell line. Contact support@diagnocine.com to request a bicarbonate-buffered variant if 5% CO₂ culture is preferred.
Yes. This is a basal medium compatible with FBS (2–10%), human serum, GlutaMAX, growth factors (EGF, FGF, VEGF), and antibiotics. Pre-filter serum and other protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter; defined, protein-free additions may be filtered at 0.1 µm. Do not use a 0.04 µm filter for supplements — it retains serum proteins and lipoproteins and clogs rapidly.
Endotoxin is controlled per manufacturing batch to a release specification of < 0.05 EU/mL, tested by LAL assay per USP <85> (assay sensitivity 0.005 EU/mL). Every batch is tested before release; lot-specific results are available on the Certificate of Analysis at support@diagnocine.com.
Yes. A full CoA per lot includes: lot number, expiry, appearance, osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), mycoplasma control statement, particulate count (USP <788>), and cultural response. Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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