FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Calcium Chloride: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Calcium Chloride: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/Ham's F-12 (3:1) medium with 15 mM HEPES, formulated without calcium chloride, and engineered for microfluidic channels, organ-on-a-chip (OoC) and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Approximately 5× lower sub-visible particulate count than conventional 0.22 µm-filtered media (USP <788> Method 1).

• Quadruple-stage filtration to a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2), engineered for organ-on-a-chip and microfluidic perfusion
• 3:1 DMEM/Ham's F-12 base with 15 mM HEPES and 3070 mg/L sodium bicarbonate for dual-buffered pH stability on and off the bench
• 3.8255 g/L D-glucose, 474.500 mg/L L-glutamine and 27.525 mg/L sodium pyruvate provide a complete, ready-to-use carbon and nitrogen source
• Formulated without calcium chloride — suited to calcium-sensitive assays, dissociation protocols and calcium-signalling studies
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per batch prior to release
• Manufactured under an ISO 13485:2016 quality management system with ISO Class 5 aseptic fill & finish
• Custom pH, glucose, HEPES, salts and nutrient adjustments available on request — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and compromising long-duration perfusion. FluxMPS™ closes that gap.

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — two dedicated prefilter + final-filter pairs run in series for full redundancy.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final-filter cartridge.

2. 2

   0.04 µm Final filtration I

   Retains fine particulates and microaggregates, and provides mycoplasma-retentive filtration (typical mycoplasma diameter 0.2–0.3 µm).

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge and enabling full-train redundancy.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Calcium Chloride: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactor and robotic liquid-handling platforms requiring the highest particulate exclusion.

• Total Particulate Exclusion — six-stage 0.01 µm cascade
• Valve & Sensor Protection — minimizes fouling in automated fluidics
• Extended Perfusion Stability — supports unattended long-duration runs

Inquiry Required: the 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com.

Full technical specification

Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Calcium Chloride: 1X Liquid is released against multi-parameter QC specifications. Available pack sizes: 500 mL, 1000 mL.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 51 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others). All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

How DCP-DMF12H-CT1X compares

Comparison of filtration, mycoplasma barrier, water quality, and endotoxin release specification against published supplier specifications for conventional 0.22 µm filtered media.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 15mM HEPES w/o Calcium Chloride: 1X Liquid (DCP-DMF12H-CT1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x