FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1): 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1): 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM/F-12 (3:1) engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Formulated with sodium bicarbonate, L-glutamine, sodium pyruvate and a phenol red pH indicator for robust, chemically defined culture performance.

• Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) with a 0.04 µm final polish, engineered for microfluidic channel compatibility
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
• DMEM/F-12 (3:1) formulation with 3825.5 mg/L glucose, 474.5 mg/L L-glutamine, 27.525 mg/L sodium pyruvate, and 3070 mg/L sodium bicarbonate
• Phenol red pH indicator (12.235 mg/L) included for visual pH monitoring
• Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
• 0.1 µm mycoplasma-retentive filtration stages (mycoplasma organisms typically 0.2–0.3 µm) — a filtration-based control, not a per-lot mycoplasma assay
• Custom pH, glucose, HEPES, salts & nutrients available — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and complicating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

A validated four-pass sequential filtration train reaching a final pore size of 0.04 µm, run as two dedicated prefilter + final-filter pairs rather than a single descending cascade.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

2. 2

   0.04 µm Final filtration I

   Retains sub-micron particulates and microaggregates, including the mycoplasma size range (0.2–0.3 µm), that pass a standard 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   A second, dedicated prefilter protecting the second 0.04 µm cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1): 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are important.

Automated Bioreactors & Robotics

For automated perfusion systems and robotic bioreactor platforms, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation.

• Total Particulate Exclusion — six-stage cascade down to 0.01 µm
• Valve & Sensor Protection — minimizes fouling in automated fluidic hardware
• Extended Perfusion Stability — supports longer unattended run times

Inquiry Required: the 0.01 µm MPS Grade variant is produced to order — contact support@diagnocine.com to discuss availability for this formulation.

Full technical specification

Every lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1): 1X Liquid is released against comprehensive multi-parameter QC specifications.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 51 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

How DCP-DMF12-T1X compares

Advantages in filtration architecture, mycoplasma-retentive filtration, water quality, and QC depth relative to conventional DMEM/F-12 media for MPS and microfluidic applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1): 1X Liquid (DCP-DMF12-T1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x