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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-DMEMH-B1X is a Microfluidics Suitable, ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and CO2-independent or open-top microfluidic platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO2-independent pH buffering. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Bicarbonate.
- High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and other aerobically active cell types
- 25 mM HEPES (pKa 7.3 at 37°C) provides robust pH buffering independent of CO2 tension, for open-top and CO2-free platforms
- Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final cut-off for microfluidic channels below 100 µm
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- Contains phenol red sodium salt (15.9 mg/L) as a pH indicator; a phenol red–free variant is available for autofluorescence-sensitive imaging
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Sodium bicarbonate intentionally excluded — HEPES alone maintains pH 7.2–7.4 at 37°C without CO2 supplementation
- Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
- Formulation[+] Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] HEPES, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Bicarbonate
- AppearanceOrange-colored (phenol red indicator), clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)250–290 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- FiltrationQuadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized organisms, subvisible particulates, and does not buffer pH once chips are handled outside a CO2 incubator. FluxMPS™ addresses both failure modes simultaneously.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion in sub-100 µm channel geometries.
High-energy cell support
4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.
HEPES: CO2-stable pH
25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.2–7.4 regardless of CO2 fluctuation — useful for open-top chips, point-of-care devices, and atmospheric incubation.
Low background for imaging
Ultra-low particulate baseline from 0.04 µm final filtration suits confocal and biosensor platforms on chip. This formulation contains phenol red as a pH indicator; a phenol red–free variant is available on request for autofluorescence-sensitive assays.
Rich, stable nutrient profile
33 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.
Customization on demand
pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — a repeated 0.1 µm prefilter + 0.04 µm final-filter pair, run twice in series — reaching a final 0.04 µm polish under ISO Class 5 aseptic fill.
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1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates and contaminants; protects the first 0.04 µm cartridge and downstream chip geometries.
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2
0.04 µm Final Filtration I — Mycoplasma-Retentive Filtration
First 0.04 µm pass; retains mycoplasma-sized organisms (0.2–0.3 µm) and fine particulates not captured by standard 0.22 µm filtration.
-
3
0.1 µm Prefiltration II — Second-Pass Protection
Dedicated second prefilter protecting the second 0.04 µm cartridge in the paired filtration train.
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4
0.04 µm Final Filtration II — Polish
Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.
Performance vs. conventional media
© Diagnocine® — DCP-DMEMH-B1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMH-B1X combines high-glucose energy support with HEPES pH stability — supporting platforms from open-top microfluidic chips and CO2-free bioreactors to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
HEPES buffering removes CO2 dependency in automated bioreactor perfusion. An optional 0.01 µm (10 nm) MPS Grade variant — the same six-stage ultra nano-filtered line referenced in the Grade note above — is available on request for robotic liquid handlers where trace particulates cause valve failure.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates from bioreactor media lines
- pH-Stable Automated Delivery: HEPES maintains pH during robotic media exchanges without CO2 re-equilibration delays
- Extended Perfusion Stability: Consistent high-glucose delivery with stable pH over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Open-Top & CO2-Free Chips
HEPES buffering enables pH-stable culture in open-top microfluidic devices, atmospheric incubators, and multi-compartment chips with heterogeneous CO2 environments.
Primary Neurons & Brain-on-Chip
High glucose (4.5 g/L) plus HEPES stability supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion without pH drift.
Cardiomyocyte & Heart-on-Chip
High-glucose DMEM plus HEPES is a common base for iPSC-CM maturation and heart-on-chip functional assays requiring stable pH and high energy substrate.
Warburg Effect & Cancer Models
High glucose supports aerobic glycolysis in cancer lines; HEPES stabilizes pH during rapid glucose consumption spikes in Warburg-active tumour models.
Metabolic Flux Analysis
Defined high-glucose formulation for 13C isotope tracing and NMR metabolomics; HEPES allows sampling outside incubators without pH artefacts. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium.
Microscopy & Optical Sensing
HEPES-stabilized pH supports extended live-cell imaging sessions outside CO2 incubators. This formulation contains phenol red as a pH indicator; request our phenol red–free variant for applications requiring minimal optical background.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
Available pack sizes: 500 mL, 1000 mL.
| Parameter | Specification |
|---|---|
| Formulation | [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Bicarbonate |
| Appearance | Orange-colored (phenol red indicator), clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 250–290 mOsm/kg H2O |
| Total ingredients | 33 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 33 ingredients verified per lot with CAS numbers for raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the OTHERS group.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 7.200 |
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified facilities. Final QA and testing at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity feedwater; trace-metal and organic-carbon (TOC) control minimizes lot-to-lot raw-material variability.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every batch — no blending, no averaged QC results.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 250–290 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every batch on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMH-B1X compares
FluxMPS™ DCP-DMEMH-B1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMH-B1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG + HEPES (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard (0.22 µm filtered), no grade tier | Standard (0.22 µm filtered), no grade tier |
| HEPES-only buffering — fully CO2-independent, atmospheric incubation compatible | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1/0.04 µm train) | cancel No | cancel No |
| HEPES buffer (25 mM) | check_circle Yes | cancel No | check_circle Yes |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested (Method 1) | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMH-B1X DMEM High Glucose + 25mM HEPES.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
