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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEML-PB1X
$34.10
DCP-DMEML-PB1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains Low Glucose Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Without Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-DMEML-PB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM Low Glucose formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. Processed through a validated four-stage filtration train reaching a 0.04 µm final polish, it supports unobstructed microchannel flow, 0.1 µm mycoplasma-retentive filtration, and low-background optical measurements. Formulation: [+] Low Glucose, [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate.

  • Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final cut-off
  • Low Glucose (1.0 g/L) base formulated without sodium pyruvate and without sodium bicarbonate — suited to CO₂-independent or user-buffered microfluidic culture
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch prior to release
  • Contains phenol red (15.900 mg/L) as a visual pH indicator — this is not a phenol red–free formulation
  • 4× Basal Medium Eagle (BME) amino acid and vitamin concentrations across 31 total ingredients
  • Manufactured under an ISO 13485:2016 quality management system with per-lot Certificate of Analysis
  • Custom pH, glucose concentration, salts, and nutrient adjustments available on request
DCP-DMEML-PB1X | Size: 500 mL and 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-Glutamine584 mg/L
  • Sodium PyruvateNot included
  • Sodium BicarbonateNot included
  • Phenol Red15.900 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage / Shelf Life2–8°C, protect from light / 12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma (0.2–0.3 µm diameter), subvisible particulates, and endotoxin fragments that accumulate inside microfluidic channels, clog chip geometries, and corrupt sensor readings and metabolic signals. FluxMPS™ was designed specifically for these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration removes sub-micron particles that clog channels below 100 µm. USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion in fine chip geometries.

target

Total metabolic control

Low glucose (1.0 g/L) base supplied without sodium pyruvate and without sodium bicarbonate lets researchers define the carbon source and buffer system precisely for Warburg-effect and metabolic flux studies.

water_drop

Ultrapure-grade water

Every batch is prepared using Ultrapure Type 1 water (18.2 MΩ·cm), supporting low trace-metal and organic-carbon background in the finished medium.

visibility

Low background for imaging

Ultra-low particulate count and (in phenol red–free variants) no autofluorescent dye delivers a cleaner particulate baseline for confocal, widefield, and biosensor measurements on chip.

science

Rich, stable nutrient profile

4× BME amino acid & vitamin concentrations, micro-batch manufacturing, and lot-to-lot QC support reproducible cell growth across multi-day perfusion experiments.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and nutrient composition are adjustable per your protocol. Contact support@diagnocine.com to specify your formulation.

Purity Architecture

Quadruple-stage filtration system

A validated quadruple-stage filtration train reaching a final 0.04 µm polish, engineered to exclude sub-mycoplasma particulates from microfluidic channels while preserving full nutrient integrity.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and contaminants; protects the first 0.04 µm final filter and extends its service life.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter does not.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated 0.1 µm prefilter protecting the second 0.04 µm cartridge — the pairing runs in series, not as a single descending cascade.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish, completing the quadruple-stage purity architecture.

Performance vs. conventional media

FluxMPS™ DCP-DMEML-PB1X is processed to a materially finer specification than standard 0.22 µm–filtered DMEM, addressing microchannel fouling and particulate-driven signal noise in sensitive MPS workflows.

4
Sequential filtration
passes (0.1 µm ×2 +
0.04 µm ×2)
0.04
µm Final filtration
pore size — sub-mycoplasma
polishing
Sterility & Mycoplasma: No bacterial or fungal growth observed after 14 days incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms typically measure 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEML-PB1X Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid - Quadruple-stage filtration system diagram showing four sequential stages: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, and 0.04 micron Final filtration II - engineered for organ-on-a-chip and microfluidic MPS cell culture media | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system: four serial stages (0.1 µm ×2 + 0.04 µm ×2) delivering microfluidics-suitable purity for microphysiological systems and organ-on-a-chip applications.
© Diagnocine® — DCP-DMEML-PB1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEML-PB1X supports demanding cell culture platforms — from single-channel microfluidic chips to multi-organ body-on-a-chip systems — where media purity, metabolic precision, and optical clarity matter.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available on request for automated bioreactor perfusion systems, robotic liquid handlers, and long-duration closed-loop platforms where even trace particulates cause valve failure or sensor drift.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC methods
  • Valve & Sensor Protection: Reduces micro-fouling of solenoid valves, peristaltic pump tubing, and inline optical sensors
  • Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture without filter replacement in the chip circuit

Inquiry Required: The 0.01 µm MPS Grade variant is a custom product. Contact support@diagnocine.com to request it for your automated system.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm–filtered media supports laminar flow fidelity and helps avoid microchannel clogging in complex multi-organ chip architectures.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Low glucose (1.0 g/L) base without sodium pyruvate enables precise control of aerobic glycolysis, supporting Warburg phenotype studies.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-low endotoxin (< 0.05 EU/mL release specification) and 0.1 µm mycoplasma-retentive filtration support sensitive iPSC-differentiation protocols.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-controlled, endotoxin-tested DMEM supports HUVEC and primary hepatocyte monolayer integrity, useful for barrier-function assays and TEER monitoring.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined low-glucose formulation without sodium pyruvate provides a clean metabolic background for ¹³C isotope tracing and extracellular flux measurement. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate count delivers a cleaner particulate baseline for confocal imaging, biosensor arrays, and TEER measurements on chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot is released against the full specification matrix below. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Low Glucose, [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate
Appearance Orange-colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
L-Glutamine 584 mg/L
Sodium Pyruvate Not included
Phenol Red 15.900 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Not required — bicarbonate-free formulation; use HEPES or an equivalent buffer for CO2-independent culture
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

FluxMPS™ DMEM is a modification of Basal Medium Eagle (BME) containing 4× BME concentrations of amino acids and vitamins, plus glycine, serine, and ferric nitrate. Total ingredients: 31. Every ingredient listed below is present in the released product; CAS numbers provided for traceability.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
i-Inositol 87-89-8 7.200
OTHERS
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 15.900
Custom formulation: pH, glucose concentration, salts, HEPES, and nutrient composition are available on request. Contact support@diagnocine.com for custom co-formulation of DCP-DMEML-PB1X.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. All final packaging, QA, and testing are conducted at the Diagnocine R&D and Quality Testing Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

All formulations use Ultrapure Type 1 water (18.2 MΩ·cm) to minimize trace-metal and organic-carbon background across every batch.

biotech

ISO Class 5 Fill & Finish

Final aseptic fill performed in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity at the point of fill.

assignment

Micro-Batch Precision

Small-batch production with full per-lot traceability. Each batch is individually tested and released — no blending of lots, no averaging of QC results. A Certificate of Analysis is issued for every batch.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay. Release specification: < 0.05 EU/mL. Every manufacturing batch is tested prior to release.

Particulate — USP <788> Method 1

Light obscuration particle count. Release limits: NMT 25/mL (≥10 µm) and NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target range: 310–350 mOsm/kg H2O.

Documentation & CoA

Full Certificate of Analysis with raw-material traceability, in-process records, and final-release test results available for every batch upon request.

Certificate of Analysis: Request the CoA for any lot of DCP-DMEML-PB1X by emailing support@diagnocine.com.
Product Comparison

How DCP-DMEML-PB1X compares

FluxMPS™ DCP-DMEML-PB1X vs. conventional 0.22 µm–filtered DMEM formulations.

Parameter DCP-DMEML-PB1X (FluxMPS™) Conventional DMEM
(0.22 µm filtered)		 Standard Alt. DMEM
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm) Standard grade Standard grade
No sodium pyruvate, no sodium bicarbonate — supports CO2-independent systems with a researcher-defined carbon source check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel Not tested cancel Not tested
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed formulation cancel Fixed formulation

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEML-PB1X and Microfluidics Suitable DMEM formulations.

Yes. DCP-DMEML-PB1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, supporting low particulate levels that help prevent microchannel clogging. It is a Microfluidics Suitable product engineered for microphysiological systems (MPS), OoC, tissue-on-a-chip (ToC), and lab-on-a-chip (LoC) platforms where sub-micron particle accumulation can cause flow obstruction and signal artifacts.
Conventional 0.22 µm filtration retains bacteria but does not target mycoplasma (0.2–0.3 µm) or finer subvisible particulates. FluxMPS™ uses four sequential filters: 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II, reaching a 0.04 µm final cut-off with 0.1 µm mycoplasma-retentive filtration at each pass.
Sodium bicarbonate is omitted for use in CO2-independent or open-top microfluidic environments where HEPES (10–25 mM recommended) or another organic buffer controls pH. Sodium pyruvate is omitted so researchers can add it fresh at a defined concentration (typically 1 mM / 110 mg/L) when oxidative support is needed, without the instability of pyruvate stored in liquid media. This combination suits multi-organ chip perfusion systems where different chip compartments operate at different CO2 tensions.
No. DCP-DMEML-PB1X is supplied without sodium bicarbonate, so it does not rely on a CO2/bicarbonate buffering system. Use HEPES (10–25 mM) or another organic buffer for pH control in ambient or open microfluidic environments, eliminating the need for a CO2 incubator.
Yes. This medium is a defined base formulation. Add FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or custom nutrients as required. Filter serum-containing or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane — never 0.04 µm, which retains serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch by LAL assay per USP <85> (assay sensitivity 0.005 EU/mL). Every batch is tested before release and must meet the release specification of < 0.05 EU/mL. A Certificate of Analysis for the specific batch is available on request.
Yes. A full CoA is issued for every batch and includes: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration confirmation, particulate count (USP <788> Method 1), and raw-material traceability. Request via support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip, microfluidic, and metabolic research applications.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  7. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  8. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  9. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  10. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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