FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Sodium Pyruvate: 1X Liquid
FluxMPS™ DCP-DMEML-P1X is an MPS-grade, ultra-filtered DMEM Low Glucose formulation engineered for microphysiological systems, organ-on-a-chip (OoC), and microfluidic tissue models. Processed through a proprietary Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers particulate counts approximately 5× lower than conventional 0.22 µm–filtered media — ensuring unobstructed microchannel flow, mycoplasma-free media, and low-background optical measurements.
- 0.04 µm final nano-filtration for sub-mycoplasma particle removal — critical for chip geometries below 100 µm
- Quadruple-stage sequential filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Endotoxin < 0.05 EU/mL (LAL, USP <85>) — 20× below typical cell culture grade
- Formulation: [+] Low Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate | [-] Sodium Pyruvate
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) per USP <85>
- ISO Class 5 (Class 100) aseptic fill & finish, ISO 13485:2016 QMS
- Custom pH, glucose, salts, and nutrient adjustments available on request
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- L-Glutamine584 mg/L
- Sodium PyruvateNot included
- Sodium Bicarbonate3700 mg/L
- Phenol Red15.900 mg/L
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage / Shelf Life2–8°C / 12 months
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma (0.1–0.3 µm diameter), subvisible particulates, and endotoxin fragments that accumulate inside microfluidic channels, clog chip geometries, and corrupt sensor readings and metabolic signals. FluxMPS™ was designed specifically for these failure modes.
Microchannel-safe purity
0.04 µm final filtration removes sub-micron particles that clog channels below 100 µm. USP <788> particulate compliance ensures safe perfusion in every chip geometry.
Total metabolic control
Low glucose (1.0 g/L) base with selectable pyruvate, bicarbonate, and phenol red allows researchers to define carbon sources precisely for Warburg-effect and metabolic flux studies.
Ultrapure-grade water
Every batch is prepared using Ultrapure Type 1 water (18.2 MΩ·cm), meeting USP <85> resistivity — eliminating ionic contaminants that interfere with electrophysiology and TEER measurements.
Low background for imaging
Ultra-low particulate count and (in phenol red–free variants(SHU: DCP-DMEML-PR1X)) no autofluorescent dye delivers cleaner confocal, widefield, and biosensor baselines on chip.
Rich, stable nutrient profile
4× BME amino acid & vitamin concentrations, micro-batch manufacturing, and lot-to-lot QC ensure reproducible cell growth across multi-day perfusion experiments.
Customization on demand
pH, glucose concentration, salts, HEPES, and nutrient composition are adjustable per your protocol. Contact support@diagnocine.com to specify your formulation.
Quadruple-stage filtration system
The only ready-to-use DMEM at this purity level — four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions. No other commercially available DMEM formulation offers this level of particulate exclusion while maintaining full nutrient integrity.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates, cell debris, and contaminants. Extends the service life of downstream 0.04 µm membranes and protects chip geometries from early fouling.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains fine particulates, bacteria, and critically mycoplasma (typical diameter 0.1–0.3 µm) — a key step absent from standard 0.22 µm filtration.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass removal of residual contaminants and bioburden. Provides sterility redundancy, ensuring no breakthrough from Stage 1.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate polish for ultra-pure, particle-free, mycoplasma-free media. Final fill performed in a validated ISO Class 5 (Class 100) laminar-flow workstation under aseptic conditions.
Performance vs. conventional media
FluxMPS™ DCP-DMEML-P1X delivers measurably cleaner media than any standard 0.22 µm–filtered DMEM, addressing the root cause of microchannel fouling, signal noise, and mycoplasma contamination in sensitive MPS workflows.
0.22 µm media by
particulate count
pore size — sub-mycoplasma
polishing
© Diagnocine® — DCP-DMEML-P1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEML-P1X supports the most demanding cell culture platforms — from single-channel microfluidic chips to multi-organ body-on-a-chip systems — where media purity, metabolic precision, and optical clarity are non-negotiable.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant is available on request for automated bioreactor perfusion systems, robotic liquid handlers, and long-duration closed-loop platforms where even trace particulates cause valve failure or sensor drift.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC methods
- Valve & Sensor Protection: Eliminates micro-fouling of solenoid valves, peristaltic pump tubing, and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture without filter replacement in the chip circuit
Inquiry Required: The 0.01 µm grade is a custom product. Contact support@diagnocine.com to request this variant for your automated system.
Micro Physiological System (MPS) & Chip
Ultra-clean, 0.04 µm–filtered media prevents microchannel clogging and maintains laminar flow fidelity in complex multi-organ chip architectures.
Warburg Effect & Metabolic Research
Low glucose (1.0 g/L) base with selectable pyruvate enables precise control of aerobic glycolysis, supporting authentic Warburg phenotype studies.
iPSC-Derived Models
Ultra-low endotoxin (< 0.05 EU/mL) and mycoplasma-free certification make this medium safe for sensitive iPSC-differentiation protocols.
Endothelial & Primary Cells
Particle-free, endotoxin-controlled DMEM supports HUVEC and primary hepatocyte monolayer integrity, essential for barrier-function assays and TEER monitoring.
Metabolic Flux Analysis
Defined low-glucose formulation with optional pyruvate omission provides a clean metabolic background for ¹³C isotope tracing and extracellular flux measurement.
Microscopy & Optical Sensing
Ultra-low particulate count and optional phenol red–free variants deliver minimal autofluorescence for confocal imaging, biosensor arrays, and TEER measurements on chip.
Analytical release specifications
Every lot is released against the full specification matrix below. CoA available on request: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Low Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate | [-] Sodium Pyruvate |
| Appearance | Red-colored, clear solution |
| pH USP <791> | 7.4 ± 0.2 |
| Osmolality USP <785> | 310–350 mOsm/kg H₂O |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | Not included |
| Phenol Red | 15.900 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ (bicarbonate-containing formulations) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
FluxMPS™ DMEM is a modification of Basal Medium Eagle (BME) containing 4× BME concentrations of amino acids and vitamins, plus glycine, serine, and ferric nitrate. Total ingredients: 32. Every ingredient listed below is present in the released product; CAS numbers provided for traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous, multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. All final packaging, QA, and testing are conducted at the DiagnoCine R&D and Quality Testing Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
All formulations use only Ultrapure Type 1 water (18.2 MΩ·cm) meeting USP <85> resistivity — eliminating ionic contaminants that compromise cell signaling and sensor measurements.
ISO Class 5 Fill & Finish
Final aseptic fill performed in validated ISO Class 5 (Class 100) laminar-flow workstations, ensuring container-closure integrity and eliminating environmental contamination at the point of fill.
Micro-Batch Precision
Small-batch production with full per-lot traceability. Each batch is individually tested and released — no blending of lots, no averaging of QC results. A Certificate of Analysis is issued for every lot.
Endotoxin — USP <85> BET
Limulus Amebocyte Lysate (LAL) assay. Release limit: < 0.05 EU/mL. Tested on every production lot.
Particulate — USP <788> Method 2
Light obscuration particle count. Release limits: ≤25/mL (≥10 µm) and ≤3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target range: 310–350 mOsm/kg H₂O. Ensures physiological tonicity across all cell culture platforms.
Documentation & CoA
Full Certificate of Analysis with raw-material traceability, in-process records, and final-release test results available for every lot upon request.
How DCP-DMEML-P1X compares
FluxMPS™ DCP-DMEML-P1X vs. conventional 0.22 µm–filtered DMEM formulations.
| Parameter | DCP-DMEML-P1X (FluxMPS™) | Conventional DMEM (0.22 µm filtered) |
Standard Alt. DMEM (0.22 µm filtered) |
|---|---|---|---|
| No Sodium Pyruvate formulation — ideal for cells sensitive to pyruvate or metabolic flux studies requiring defined carbon sources | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP particulate compliance | check_circle USP <788> | cancel Not tested | cancel Not tested |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed formulation | cancel Fixed formulation |
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEML-P1X and MPS-grade DMEM formulations.
Supporting literature
Key peer-reviewed publications supporting the use of MPS-grade, ultra-filtered media in organ-on-a-chip, microfluidic, and metabolic research applications.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
