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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEMH-GBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered glucose-free DMEM + 25 mM HEPES formulation engineered for metabolic flux analysis, ¹³C isotope tracing, Warburg-effect research, and organ-on-a-chip applications where carbon source identity must be precisely controlled. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Glutamine, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Sodium Bicarbonate, [-] Phenol Red, [-] Glucose.
- Glucose-free — add D-glucose, ¹³C-glucose, galactose, fructose, or any carbon source at your defined concentration
- 25 mM HEPES (pKa 7.3 at 37°C) provides CO2-independent pH buffering — ideal for Seahorse XF, open-top chips, and metabolic sampling outside incubators
- Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final cut-off
- Endotoxin specification < 0.05 EU/mL (LAL, USP <85>) supports clean metabolic baselines for glycolysis/OCR measurements
- Formulation: [+] L-Glutamine, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Sodium Bicarbonate, [-] Phenol Red, [-] Glucose
- Manufactured under an ISO 13485:2016 quality management system with per-lot Certificate of Analysis; final QA at Diagnocine, Totowa, NJ
- Custom glucose concentrations, alternative carbon sources, and nutrient modifications available on request
- GlucoseNot included — researcher-defined
- HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
- Formulation[+] L-Glutamine, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate, [-] Sodium Bicarbonate, [-] Phenol Red, [-] Glucose
- AppearancePale yellow, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, protected from light
- Shelf Life12 months from date of manufacture, unopened
When carbon source control is the experiment
Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh at the concentration and identity you choose, while the quadruple-stage filtration and < 0.05 EU/mL endotoxin specification support clean metabolic readouts.
Complete carbon source control
Add D-glucose, ¹³C-glucose, U-¹³C₂-galactose, fructose, or no sugar. Combine with palmitate or other fatty acids to define carbon source identity and concentration precisely for every experiment.
Seahorse XF & metabolomics ready
Glucose-free base is the standard for Seahorse XF Glycolysis Stress Tests. HEPES maintains pH during real-time injection protocols without CO2 dependency — no pH drift between ports.
HEPES: CO2-stable pH
25 mM HEPES (pKa 7.3 at 37°C) maintains stable pH outside CO2 incubators — useful for metabolic sampling, Seahorse injection events, and open-top chip platforms.
Microchannel-safe purity
0.04 µm final filtration and a < 0.05 EU/mL endotoxin specification support metabolic baselines free of particulate and endotoxin confounds.
Low background for imaging
This phenol red-free formulation removes phenol red autofluorescence and estrogen-receptor agonist activity, supporting confocal imaging, TEER, and biosensor OoC platforms against a low-particulate baseline. (Riboflavin, common to all DMEM-based media, remains present.)
Customization on demand
Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions.
-
1
0.1 µm Prefiltration I
Removes large aggregates and protein aggregates; protects the first 0.04 µm cartridge from early fouling.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; pore size below the 0.2–0.3 µm mycoplasma size range, providing mycoplasma-retentive filtration (not tested per lot) and retaining sub-micron particulates beyond standard 0.22 µm filtration.
-
3
0.1 µm Prefiltration II
A second dedicated 0.1 µm prefilter, protecting the second 0.04 µm final filter cartridge.
-
4
0.04 µm Final filtration II — Polish
Final 0.04 µm polishing pass; ISO Class 5 (Class 100) aseptic fill & finish.
Why filtration purity matters for metabolic assays
Subvisible particulates and elevated endotoxin are independently associated with TLR4/NF-κB signalling that upregulates glycolysis — a potential confound for glucose uptake, lactate production, and OCR measurements. FluxMPS™ DCP-DMEMH-GBR1X is specified to < 0.05 EU/mL endotoxin and NMT 25 particles/mL (≥10 µm).
© Diagnocine® — DCP-DMEMH-GBR1X
Metabolic research and OoC applications
FluxMPS™ DCP-DMEMH-GBR1X is purpose-built for experiments where carbon source identity must be controlled — from Seahorse XF assays to ¹³C metabolic flux analysis and glucose dose-response studies on organ-on-a-chip platforms.
Seahorse XF & Real-Time Metabolic Flux
Glucose-free DMEM is the recommended base for Seahorse XF Glycolysis Stress Tests (add glucose, oligomycin, 2-DG) and Mito Stress Tests (add glucose + glutamine + pyruvate, then oligomycin, FCCP, rotenone/antimycin). The 25 mM HEPES in DCP-DMEMH-GBR1X supports pH stability between injection events without CO2 re-equilibration.
- Glycolysis Stress Test: Glucose-free base minimizes background ECAR before glucose injection
- Mito Stress Test: Add glucose + glutamine + pyruvate to defined concentrations
- ¹³C Isotope Tracing: Add U-¹³C?-glucose at exact specific activity without dilution from background glucose
Inquiry Required: Custom glucose, galactose, or ¹³C-carbon source co-formulations available. Contact support@diagnocine.com.
¹³C Metabolic Flux Analysis
Add U-¹³C?-glucose at any specific activity without background dilution. HEPES maintains pH during NMR sample preparation outside incubators.
Warburg Effect & OXPHOS Switching
Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without media changes.
Glucose Dose-Response on Chip
Perfuse defined glucose gradients through OoC channels to study dose-dependent metabolic responses in endothelial, beta-cell, or hepatocyte chips.
iPSC Metabolic Maturation
Glucose-free base enables galactose-forced OXPHOS maturation of iPSC-derived cardiomyocytes and hepatocytes.
Nutrient Deprivation Studies
Starvation experiments, glucose withdrawal, and nutrient re-feeding protocols with defined timing and concentration control.
Metabolic Imaging & Biosensors
This phenol red-free formulation removes phenol red autofluorescence for FLIM, NAD(P)H imaging, glucose FRET sensors, and TEER measurements on metabolic chips.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Sodium Bicarbonate, [-] Phenol Red, [-] Glucose |
| Appearance | Pale yellow, clear solution |
| Glucose | Not included — add at desired concentration |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H2O |
| Total ingredients | 31 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm/0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | CO2-independent — 25 mM HEPES alone maintains pH 7.2–7.4 at 37°C without gas supplementation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Pack sizes: 500 mL, 1000 mL
Full composition (mg/L)
Glucose-free DMEM + HEPES scaffold: 31 ingredients verified per lot with CAS numbers. Glucose is intentionally absent — add your carbon source of choice. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| Sodium pyruvate | 113-24-6 | 110.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm ultrapure water supports low trace-metal and total organic carbon (TOC) background for metabolic flux measurements.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending between batches, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: 310–350 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMH-GBR1X compares
FluxMPS™ DCP-DMEMH-GBR1X vs. conventional glucose-free DMEM and standard DMEM for metabolic assays.
| Parameter | DCP-DMEMH-GBR1X (FluxMPS™) | Conventional GF-DMEM (0.22 µm filtered) |
Standard DMEM (fixed glucose, 0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm) | Standard grade (0.22 µm) | |
| Glucose-free DMEM + HEPES — no Sodium Bicarbonate, no Phenol Red; CO2-independent with pyruvate and glutamine included | check_circle Yes | cancel No | cancel No |
| Glucose content | None — researcher-defined | None | Fixed (1–4.5 g/L) |
| HEPES (25 mM) | check_circle Yes | cancel Usually no | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Mycoplasma-retentive filtration | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Seahorse XF compatible | check_circle Yes (GF base + HEPES) | check_circle Yes (no HEPES) | cancel Carbon source fixed |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMH-GBR1X glucose-free DMEM + 25 mM HEPES.
Supporting literature
Key publications supporting glucose-free DMEM in metabolic flux analysis, Seahorse XF assays, isotope tracing, and organ-on-a-chip metabolic studies.
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
- Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
- Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
- Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
