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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Bicarbonate, Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEMH-GBR1X is an MPS-grade, ultra-filtered glucose-free DMEM + 25 mM HEPES formulation engineered for metabolic flux analysis, ¹³C isotope tracing, Warburg-effect research, and any organ-on-a-chip application where carbon source identity must be precisely controlled. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] Glucose, [-] Sodium Bicarbonate, [-] Phenol Red.
- Glucose-free — add D-glucose, ¹³C-glucose, galactose, fructose, or any carbon source at your defined concentration
- 25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO₂; ideal for Seahorse XF, open-top chips, and metabolic sampling outside incubators
- 0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin for clean metabolic baselines
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Formulation: [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] Glucose, [-] Sodium Bicarbonate, [-] Phenol Red
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Custom glucose concentrations, carbon sources, and nutrients available on request
- GlucoseNot included — researcher-defined
- HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
- Formulation[+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES, [-] Glucose, [-] Sodium Bicarbonate, [-] Phenol Red
- AppearancePale Yellow-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)310.00–350.00 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months
When carbon source control is the experiment
Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh at the concentration and identity you choose — while ultra-low particulate delivery ensures chip integrity and < 0.05 EU/mL endotoxin prevents inflammatory artefacts in metabolic readouts.
Complete carbon source control
Add D-glucose, ¹³C-glucose, U-¹³C?-galactose, fructose, or no sugar. Combine with palmitate or other fatty acids. Define carbon source identity and concentration precisely for every experiment.
Seahorse XF & metabolomics ready
Glucose-free base is the Seahorse XF standard for Glycolysis Stress Tests. HEPES maintains pH during real-time injection protocols without CO₂ dependency — no pH drift between ports.
HEPES: CO₂-stable pH
25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.2–7.4 outside CO₂ incubators — critical for metabolic sampling, Seahorse injection events, and open-top chip platforms.
Microchannel-safe purity
0.04 µm final filtration; < 0.05 EU/mL endotoxin ensures metabolic baselines are not confounded by LPS-driven inflammatory glycolysis.
Imaging-compatible variants
Phenol red–free options eliminate autofluorescence and estrogen-receptor agonist activity for live-cell imaging, TEER, and biosensor OoC platforms.
Customization on demand
Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions. Critical for glucose-free metabolic assays where particulate contamination and endotoxin would independently activate innate immune metabolic reprogramming.
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1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates; protects downstream 0.04 µm membranes and chip geometries from early fouling.
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2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) and fine particulates — absent from standard 0.22 µm filtration.
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3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
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4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Why filtration purity matters for metabolic assays
Subvisible particulates and endotoxin (> 0.1 EU/mL) independently upregulate glycolysis via TLR4/NF-κB signalling — confounding glucose uptake, lactate production, and OCR measurements. FluxMPS™ DCP-DMEMH-GBR1X eliminates both variables: < 0.05 EU/mL endotoxin and < 25 particles/mL (≥10 µm).
© Diagnocine® — DCP-DMEMH-GBR1X
Metabolic research and OoC applications
FluxMPS™ DCP-DMEMH-GBR1X is purpose-built for experiments where carbon source identity must be controlled — from classic Seahorse XF assays to advanced ¹³C metabolic flux analysis and glucose-dose response studies on organ-on-a-chip platforms.
Seahorse XF & Real-Time Metabolic Flux
Glucose-free DMEM is the manufacturer-recommended base for Seahorse XF Glycolysis Stress Tests (add glucose, oligomycin, 2-DG) and Mito Stress Tests (add glucose + glutamine + pyruvate, then oligomycin, FCCP, rotenone/antimycin). The 25 mM HEPES in DCP-DMEMH-GBR1X prevents pH drift between injection events without CO₂ re-equilibration.
- Glycolysis Stress Test: Glucose-free base ensures zero background ECAR before glucose injection
- Mito Stress Test: Add glucose + glutamine + pyruvate to defined concentrations — no contamination from pre-existing carbon sources
- ¹³C Isotope Tracing: Add U-¹³C?-glucose at exact specific activity without dilution from background glucose
Inquiry Required: Custom glucose, galactose, or ¹³C-carbon source co-formulations available. Contact support@diagnocine.com.
¹³C Metabolic Flux Analysis
Add U-¹³C?-glucose at any specific activity without background dilution. HEPES maintains pH during NMR sample preparation outside incubators.
Warburg Effect & OXPHOS Switching
Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without media changes.
Glucose Dose-Response on Chip
Perfuse defined glucose gradients through OoC channels to study dose-dependent metabolic responses in endothelial, beta-cell, or hepatocyte chips.
iPSC Metabolic Maturation
Glucose-free base enables galactose-forced OXPHOS maturation of iPSC-derived cardiomyocytes and hepatocytes, improving metabolic phenotype fidelity.
Nutrient Deprivation Studies
Starvation experiments, glucose withdrawal, and nutrient re-feeding protocols with defined timing and concentration control.
Metabolic Imaging & Biosensors
Phenol red–free variants deliver zero autofluorescence for FLIM, NAD(P)H imaging, glucose FRET sensors, and TEER measurements on metabolic chips.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] Glucose, [-] Sodium Bicarbonate, [-] Phenol Red |
| Appearance | Pale Yellow-colored, clear solution |
| Glucose | Not included — add at desired concentration |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| pH USP <791> | 7.4 ± 0.04 |
| Osmolality USP <785> | 310.00–350.00 mOsm/kg H₂O |
| Total ingredients | 30 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Glucose-free DMEM + HEPES scaffold: 30 ingredients verified per lot with CAS numbers. Glucose is intentionally absent — add your carbon source of choice. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| Sodium pyruvate | 113-24-6 | 110.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — eliminates ionic contaminants that would independently affect metabolic flux measurements.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending between batches, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay < 0.05 EU/mL — below TLR4 activation threshold that confounds glycolysis/OXPHOS assays.
Particulate — USP <788> Method 2
Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Target: 310.00–350.00 mOsm/kg H₂O. Glucose-free base has lower osmolality than standard DMEM; add carbon sources to adjust.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-DMEMH-GBR1X compares
FluxMPS™ DCP-DMEMH-GBR1X vs. conventional glucose-free DMEM and standard DMEM for metabolic assays.
| Parameter | DCP-DMEMH-GBR1X (FluxMPS™) | Conventional GF-DMEM (0.22 µm filtered) |
Standard DMEM (fixed glucose, 0.22 µm) |
|---|---|---|---|
| Glucose-free DMEM + HEPES — no Sodium Bicarbonate, no Phenol Red; CO₂-independent and optically clean with pyruvate and glutamine included | check_circle Yes | cancel No | cancel No |
| Glucose content | None — researcher-defined | None | Fixed (1–4.5 g/L) |
| HEPES (25 mM) | check_circle Yes | cancel Usually no | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Mycoplasma barrier | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Seahorse XF compatible | check_circle Yes (GF base + HEPES) | check_circle Yes (no HEPES) | cancel Carbon source fixed |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMH-GBR1X glucose-free DMEM + 25 mM HEPES.
Supporting literature
Key publications supporting glucose-free DMEM in metabolic flux analysis, Seahorse XF assays, isotope tracing, and organ-on-a-chip metabolic studies.
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
- Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
- Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
- Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
