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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEMH-GQB1X
$44.00
DCP-DMEMH-GQB1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid

Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate Without Glucose

FluxMPS™ DCP-DMEMH-GQB1X is a Microfluidics Suitable, ultra-filtered glucose-free DMEM + 25 mM HEPES formulation engineered for metabolic flux analysis, ¹³C isotope tracing, Warburg-effect research, and organ-on-a-chip applications where carbon source identity must be precisely controlled. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Note: this formulation contains phenol red and is not recommended for Agilent Seahorse XF assays, which require phenol-red-free medium — contact us for the compatible variant. Formulation: [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Glucose, [-] L-Glutamine, [-] Sodium Bicarbonate.

  • Glucose-free — add D-glucose, ¹³C-glucose, galactose, fructose, or any carbon source at your defined concentration
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO₂-independent pH stability; suited to open-top chips and metabolic sampling outside incubators
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off for microchannel-safe purity
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>) for clean metabolic baselines
  • Formulation: [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Glucose, [-] L-Glutamine, [-] Sodium Bicarbonate
  • Contains phenol red as a visual pH indicator — for phenol-red-free imaging or Seahorse XF work, contact support@diagnocine.com for the compatible variant
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom glucose concentrations, carbon sources, and nutrients available on request
CAT. NO.
DCP-DMEMH-GQB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid
  • GlucoseNot included — researcher-defined
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Glucose, [-] L-Glutamine, [-] Sodium Bicarbonate
  • AppearanceOrange-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310 - 350 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

When carbon source control is the experiment

Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh at the concentration and identity you choose, while ultra-low particulate delivery supports chip integrity and a < 0.05 EU/mL endotoxin release specification helps avoid inflammatory artefacts in metabolic readouts.

science

Complete carbon source control

Add D-glucose, ¹³C-glucose, U-¹³C6-galactose, fructose, or no sugar. Combine with palmitate or other fatty acids. Define carbon source identity and concentration precisely for every experiment.

analytics

Metabolic flux & isotope tracing

Glucose-free base lets you add U-¹³C6-glucose, galactose, or fructose at defined specific activity for flux analysis, NMR metabolomics, and LC-MS/MS workflows without background dilution from endogenous glucose.

water_drop

HEPES: CO₂-stable pH

25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.2–7.4 outside CO₂ incubators — useful for metabolic sampling and open-top chip platforms.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and a < 0.05 EU/mL endotoxin release specification support clean metabolic baselines for glucose uptake, lactate, and OCR measurements.

visibility

Imaging-compatible variants

Phenol red–free options reduce autofluorescence and estrogen-receptor agonist activity for live-cell imaging, TEER, and biosensor OoC platforms. This SKU contains phenol red; contact us for a phenol-red-free variant.

tune

Customization on demand

Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under aseptic fill conditions — two dedicated prefilter + final-filter pairs run in series for redundant assurance.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and protein aggregates; protects the first 0.04 µm final filter from early fouling.

  2. 2

    0.04 µm Final filtration I

    Retains mycoplasma (0.2–0.3 µm) and fine particulates — not achievable with standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm cartridge, providing redundant assurance.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish.

Why filtration purity matters for metabolic assays

Elevated particulate counts and endotoxin are known to activate innate immune signalling (e.g., TLR4/NF-κB) that can upregulate glycolysis independently of experimental treatment, confounding glucose uptake, lactate production, and OCR measurements. FluxMPS™ DCP-DMEMH-GQB1X is manufactured to a batch release specification of < 0.05 EU/mL endotoxin (USP <85>) and processed through the Quadruple-stage filtration train described above.

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEMH-GQB1X Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine, Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I (mycoplasma-retentive), 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? Microfluidics Suitable glucose-free DMEM with HEPES for organ-on-a-chip and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DMEMH-GQB1X
Applications

Metabolic research and OoC applications

FluxMPS™ DCP-DMEMH-GQB1X is purpose-built for experiments where carbon source identity must be controlled — from ¹³C metabolic flux analysis to glucose-dose response studies on organ-on-a-chip platforms.

Metabolic Flux Analysis & Isotope Tracing

Precision Carbon Source Control

Glucose-free DMEM + HEPES gives full control over carbon source identity for ¹³C metabolic flux analysis, Warburg-vs-OXPHOS switching studies, and NMR/LC-MS metabolomics workflows. The 25 mM HEPES buffer maintains stable pH during extended sample handling outside CO₂ incubators.

  • ¹³C Isotope Tracing: Add U-¹³C6-glucose at exact specific activity without dilution from background glucose
  • Warburg/OXPHOS Switching: Add glucose, galactose, or no sugar to control glycolytic vs. oxidative flux
  • NMR & LC-MS Metabolomics: HEPES buffering supports stable pH during extended sample processing outside incubators

Inquiry Required: Custom glucose, galactose, or ¹³C-carbon source co-formulations available. Contact support@diagnocine.com.

Metabolomics

¹³C Metabolic Flux Analysis

Add U-¹³C6-glucose at any specific activity without background dilution. HEPES maintains pH during NMR sample preparation outside incubators.

¹³C tracingNMR metabolomicsLC-MS/MS
Cancer Biology

Warburg Effect & OXPHOS Switching

Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without media changes.

MCF-7MDA-MB-231HeLaA549
Microfluidics

Glucose Dose-Response on Chip

Perfuse defined glucose gradients through OoC channels to study dose-dependent metabolic responses in endothelial, beta-cell, or hepatocyte chips.

OoCToCDiabetes-on-chipLiver-on-chip
Stem Cell Biology

iPSC Metabolic Maturation

Glucose-free base enables galactose-forced OXPHOS maturation of iPSC-derived cardiomyocytes and hepatocytes, improving metabolic phenotype fidelity.

iPSC-CMiPSC-HepiPSC-β cells
Nutrient Biology

Nutrient Deprivation Studies

Starvation experiments, glucose withdrawal, and nutrient re-feeding protocols with defined timing and concentration control.

AutophagymTOR signalingAMPK activation
Live-Cell Imaging

Metabolic Imaging & Biosensors

Phenol red–free variants reduce background autofluorescence for FLIM, NAD(P)H imaging, glucose FRET sensors, and TEER measurements on metabolic chips. This SKU contains phenol red.

FLIMGlucose sensorsTEERConfocal
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] Glucose, [-] L-Glutamine, [-] Sodium Bicarbonate
Appearance Orange-colored, clear solution
Glucose Not included — add at desired concentration
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 310 - 350 mOsm/kg H₂O
Total ingredients 31 (across 4 categories)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (per-batch release spec — see §Manufacturing)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation
Pack sizes 500 mL, 1000 mL
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glucose-free DMEM + HEPES scaffold: 31 ingredients verified per lot with CAS numbers, across 4 category groups. Glucose is intentionally absent — add your carbon source of choice. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
Phenol red sodium salt 34487-61-1 15.900
Sodium pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Carbon source supplementation: Glucose is not included. For standard culture add D-glucose (sterile-filtered stock) at 1000 mg/L (low) or 4500 mg/L (high). For ¹³C tracing add U-¹³C6-glucose. For OXPHOS forcing add D-galactose (2000 mg/L). Contact support@diagnocine.com for custom carbon source pre-formulations.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water supports low trace-metal and organic-carbon background for metabolic flux measurements.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending between batches, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310 - 350 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GQB1X compares

FluxMPS™ DCP-DMEMH-GQB1X vs. conventional glucose-free DMEM and standard DMEM for metabolic assays.

Parameter DCP-DMEMH-GQB1X (FluxMPS™) Conventional GF-DMEM
(0.22 µm filtered)		 Standard DMEM
(fixed glucose, 0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
Glucose-free DMEM + HEPES — no Glucose, no L-Glutamine, no Sodium Bicarbonate; CO₂-independent pH check_circle Yes cancel No cancel No
Glucose content None — researcher-defined None Fixed (1–4.5 g/L)
HEPES (25 mM) check_circle Yes cancel Usually no cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (0.1 µm ×2 + 0.04 µm ×2) 1 1
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Mycoplasma barrier filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
Water quality Ultrapure Type 1, 18.2 MΩ·cm Not specified Not specified
Agilent Seahorse XF compatible cancel No — contains phenol red check_circle Typically yes (phenol-red-free) cancel Fixed carbon source
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-GQB1X glucose-free DMEM + 25 mM HEPES.

Yes. The Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) and < 0.05 EU/mL endotoxin release specification support microchannel-safe delivery for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and other microphysiological system (MPS) platforms where particulate load and endotoxin must be minimized.
The medium passes through two dedicated 0.1 µm prefilter + 0.04 µm final-filter pairs (four stages total), reaching a 0.04 µm final cut-off versus the 0.22 µm typical of conventional cell culture media. This delivers approximately 5× lower particulate counts by count and retains mycoplasma-sized organisms (0.2–0.3 µm) that a 0.22 µm filter does not.
Glucose is removed to give researchers complete control over carbon source identity and concentration — essential for ¹³C-glucose tracer studies, fatty acid oxidation experiments, and cancer metabolic reprogramming studies. Add D-glucose (0–4500 mg/L), galactose, fructose, or no sugar at all depending on your metabolic question. L-glutamine is omitted for fresh addition at time of use; sodium bicarbonate is removed so HEPES alone provides CO₂-independent pH buffering.
No. This medium is CO₂-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation, which is useful for open-top chips and metabolic sampling outside standard incubators.
Yes. Serum and other protein-containing supplements should be sterile-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — never through a 0.04 µm membrane, which retains IgM, lipoproteins, and much of the functional serum fraction. Defined, protein-free additions (e.g., glucose stocks) may use a 0.1 µm filter. Note: this formulation contains phenol red and is not recommended for Agilent Seahorse XF assays.
FluxMPS™ DCP-DMEMH-GQB1X is produced to a batch release specification of < 0.05 EU/mL by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release, and a Certificate of Analysis is available on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting glucose-free DMEM in metabolic flux analysis, isotope tracing, and organ-on-a-chip metabolic studies.

  1. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  2. Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
  3. Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
  4. Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
  5. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  7. Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
  8. Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
  9. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x

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