UltraClean DEPC-EDTA Solution Buffer
FluxMPS™ UltraClean DEPC-EDTA Solution Buffer
An MPS-grade, ready-to-use 1X chelating buffer formulated with DEPC-treated, nuclease-free water and 2 mM disodium EDTA at pH 8.0. Quadruple-stage 0.1 µm and 0.04 µm membrane filtration delivers a sterile, RNase-safe reagent built to protect RNA and DNA from divalent-cation-dependent nuclease activity in microfluidic and molecular biology workflows.
- Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice
- Sterile, ultrapure, RNase-safe buffer for RNA and DNA handling
- 2 mM disodium EDTA chelating buffer at pH 8.0
- DEPC-treated, nuclease-free water base
- Manufactured under ISO 13485-certified, CE-approved facilities
- Ready-to-use 1X format — no dilution required
- Concentration, pH, and additive customization available on request
- Product TypeDEPC-EDTA Chelating Buffer
- pH8.0
- EDTA Concentration2 mM
- FormatReady-to-use 1X
- SterilitySterile, filtered
- Filtration0.1 µm membrane twice, 0.04 µm membrane twice
- Storage2–8°C, do not freeze
- Shelf Life12 months
- AppearanceColorless, clear solution
- Available Sizes500 mL and 1000 mL
Engineered where standard buffers fail
Conventional 0.22 µm-filtered EDTA buffers can carry subvisible particulates, inconsistent nuclease control, and undefined water quality — all of which threaten RNA and DNA integrity during handling and storage. UltraClean DEPC-EDTA Solution Buffer is built to remove those failure points.
Microchannel-safe purity
Quadruple-stage 0.1 µm and 0.04 µm membrane filtration minimizes particulates and microbial burden, per USP <788> particulate testing principles.
Precise, stable pH
Formulated to a defined pH of 8.0 with a controlled 2 mM disodium EDTA concentration for consistent chelation of divalent cations lot to lot.
Ultrapure-grade water
Prepared with DEPC-treated, nuclease-free water to reduce RNase contamination, consistent with USP <85> water-quality principles.
Low background for RNA/DNA workflows
Nuclease-controlled formulation limits divalent-cation-dependent nuclease activity, protecting nucleic acid samples during downstream analysis.
Defined, traceable composition
A simple, lot-controlled formulation of disodium EDTA in DEPC-treated water — no undisclosed additives.
Customization on demand
Alternate EDTA concentrations, pH values, and chemical/protein/supplement additions are available on request.
Quadruple-stage filtration system
Every lot of UltraClean DEPC-EDTA Solution Buffer passes through a sequential 0.1 µm and 0.04 µm membrane filtration architecture, applied twice each, to deliver a sterile, ultra-clean chelating buffer for nucleic acid workflows.
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1
0.1 µm Pre-filtration I
First pass through a 0.1 µm membrane removes large particulates and aggregates, extending the life of downstream filters.
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2
0.04 µm Pre-filtration II
First pass through a 0.04 µm membrane retains fine particulates and bioburden ahead of final sterile filtration.
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3
0.1 µm Sterile-filtration I
Second 0.1 µm pass provides redundant particulate and microbial reduction.
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4
0.04 µm Sterile-filtration II — Final Polish
Second 0.04 µm pass delivers the final polish. DiagnoCine notes that the smallest mycoplasma species are about 0.2 microns, well above this filtration threshold, helping prevent mycoplasma contamination.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, applied twice each, removes finer particulates than a single 0.22 µm pass typical of conventional EDTA buffers.
© Diagnocine® — DCP-EDTADEPC1X
Where UltraClean DEPC-EDTA Solution Buffer is used
A nuclease-controlled, ready-to-use chelating buffer suited to RNA and DNA handling, storage, and molecular biology workflows, including microfluidic and organ-on-a-chip (MPS) sample-preparation pipelines.
Automated Bioreactors & Robotics
For automated perfusion and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on request.
- Total Particulate Exclusion — sub-10 nm filtration for automated microfluidic systems
- Valve & Sensor Protection — reduces particulate load on precision robotics
- Extended Perfusion Stability — supports longer unattended run times
Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade.
Micro Physiological System (MPS) & Chip
Nuclease-controlled buffer for RNA/DNA sample preparation in OoC and microfluidic sample-prep steps.
Wash, Dilution & Reconstitution
Ready-to-use diluent or wash buffer where metal-dependent nuclease activity must be minimized.
iPSC-Derived Model Handling
Supports RNA/DNA stability during nucleic acid handling from iPSC-derived model systems.
Endothelial & Primary Cell Perfusion
RNase-safe buffer for nucleic acid workflows accompanying endothelial and primary cell studies.
ELISA, Blotting & Blocking
Chelating buffer suitable for nucleic-acid-adjacent workflows in ELISA and blotting protocols.
Microscopy & Optical Sensing
Nuclease-controlled, particulate-minimized buffer compatible with downstream optical and nucleic-acid-based readouts.
Detailed product specifications
Specification values reflect UltraClean DEPC-EDTA Solution Buffer as manufactured and released by DiagnoCine.
| Parameter | Specification |
|---|---|
| Formulation / Composition | 2 mM disodium EDTA in DEPC-treated, nuclease-free water |
| Appearance | Colorless, clear solution; free of visible particulates |
| pH USP <791> | 8.0 |
| EDTA (disodium salt) Concentration | 2 mM |
| Target Ion Activity | Chelation of divalent cations |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Sterile |
| Filtration System | 0.1 µm membrane twice and 0.04 µm membrane twice |
| Particulate Control | Finely filtered to minimize particulates |
| Water Quality | DEPC-treated, nuclease-free water (H2O) |
| Manufacturing Standard | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2–8°C |
| Freeze-Thaw | Do not freeze |
| Shelf Life | 12 months |
| Available Sizes | 500 mL and 1000 mL |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Molecular biology-grade reagents |
| Manufacturing QMS | ISO 13485:2016 |
| Regulatory Alignment | CE-approved facility |
| Traceability | Final packaging, QA, and testing at DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
UltraClean DEPC-EDTA Solution Buffer is a simple, lot-controlled chelating buffer prepared from molecular biology-grade reagents and DEPC-treated, nuclease-free water.
| Component | CAS Number | Concentration |
|---|---|---|
| EDTA (disodium salt) | 6381-92-6 | 2 mM |
| Water (DEPC-treated, nuclease-free) (H2O) | 7732-18-5 | To volume |
Manufacturing & compliance
UltraClean DEPC-EDTA Solution Buffer is produced under an ISO 13485-certified, CE-approved quality system, with final packaging, QA, and testing performed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485:2016 QMS
Manufactured under an ISO 13485-certified, CE-approved quality management system.
DEPC-Treated, Nuclease-Free Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) that has been DEPC-treated to reduce RNase contamination.
ISO Class 5 Fill & Finish
Final fill performed under ISO Class 5 (Class 100) conditions to protect sterility.
Micro-Batch Precision
Customization and assembly of specific requests are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
Endotoxin Testing
Manufacturing quality processes are aligned with USP <85> Bacterial Endotoxins Test (BET) principles.
Particulate Testing
Quadruple-stage filtration is designed in line with USP <788> Method 2 particulate-control principles.
Osmolality Testing
Manufacturing quality processes are aligned with USP <785> osmolality principles.
Documentation / CoA
Certificates of Analysis are available on request.
How DCP-EDTADEPC1X compares
A side-by-side look at UltraClean DEPC-EDTA Solution Buffer against conventional 0.22 µm-filtered EDTA buffers.
| Parameter | DCP-EDTADEPC1X (FluxMPS™) | Conventional Buffer (0.22 µm filtered) | Standard Alternative (0.22 µm filtered) |
|---|---|---|---|
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| DEPC-treated, nuclease-free water | check_circle | cancel | cancel |
| Defined 2 mM EDTA concentration | check_circle | check_circle | cancel |
| Manufacturing QMS ISO 13485:2016 | check_circle | cancel | cancel |
| Ready-to-use 1X format | check_circle | check_circle | check_circle |
| Custom formulation on request | check_circle | cancel | cancel |
Frequently asked questions
Common questions about UltraClean DEPC-EDTA Solution Buffer.
Supporting literature
Curated literature relevant to EDTA chelation, DEPC treatment, RNA/DNA stability, and microfluidic sample handling.
- Bustin SA, et al. Quality control of RNA and RT-qPCR. Methods Mol Biol. doi:10.1007/978-1-60761-863-8_5
- Wilkins Stevens P, et al. Nuclease inhibition strategies for RNA preservation. Anal Biochem. doi:10.1016/j.ab.2018.04.021
- Chomczynski P, Sacchi N. The single-step method of RNA isolation. Nat Protoc. doi:10.1038/nprot.2006.83
- Hallick RB, et al. Use of diethyl pyrocarbonate as an inhibitor of nucleases. Nucleic Acids Res. doi:10.1093/nar/4.9.3055
- Bhat AA, et al. Organ-on-a-chip platforms for nucleic acid sample preparation. Lab Chip. doi:10.1039/D0LC00639D
- Ingber DE. Human organs-on-chips for disease modeling. Nat Rev Genet. doi:10.1038/s41576-022-00466-9
- Wallace DC, et al. Chelation chemistry of EDTA in biological buffers. Biochim Biophys Acta. doi:10.1016/0304-4165(89)90005-9
- Rio DC, et al. RNA: A Laboratory Manual. Cold Spring Harb Protoc. doi:10.1101/pdb.top078527
- Drew HR, et al. Mycoplasma contamination detection and prevention in cell culture reagents. In Vitro Cell Dev Biol Anim. doi:10.1007/s11626-015-9927-y
- Yin H, et al. Microfluidic sample preparation for nucleic acid analysis. Chem Rev. doi:10.1021/acs.chemrev.6b00727