Columbia Agar Base Plates/W Ampicillin Sodium Salt
Catalog No: DCP-CAPAS
pH: 7.3 ± 0.2
Uses: Standard method base for enumeration and detection of bacteria in food and environmental samples, and clinical speciments
Size: 100mm
Concentration: 30µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: light amber, clear to slightly opalescent firm gel. Adding 5% sheep (or horse/rabbit) blood, the medium becomes opaque red
Sterile
Shelf Life: 2 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish
Description:
Base Columbia agar with ampicillin sodium salt is a nutrient-rich, non-blood agar medium in which a standard Columbia agar base is supplemented with the β-lactam antibiotic ampicillin (usually as the sodium salt) to give it selective properties against ampicillin-susceptible bacteria. Columbia agar typically contains enzymatic digests of casein and meat, yeast extract, starch, and NaCl. The peptones and yeast extract provide amino acids, peptides, nitrogen, vitamins, and growth factors that support abundant growth of both fastidious and non-fastidious organisms. Starch (corn/potato) acts as a carbon source and can bind toxic metabolites, which helps recovery of delicate organisms, while NaCl maintains osmotic balance and agar provides the solid support for colony growth.
When ampicillin sodium salt is added, the medium becomes selective because ampicillin interferes with bacterial cell wall synthesis. As a β-lactam, ampicillin preferentially inhibits many Gram-positive and some Gram-negative organisms that do not carry β-lactamases or other resistance mechanisms.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
