Cat. No. FNK-CTN-50-01 Size 1 ml Lot No. 1-S001A Protein conc. 1.4 mg/ml Volume 1 ml Form 20 mM Tris-HCl(pH 7.5) Storage -20 oC *Avoid the freeze and thaw. Activity 83.0 U/ml Notes For research use only
Description
EC No.:3.2.1.14 Substrate: chitin Reaction product :chitobiose Reaction temperature : - Reaction pH : 3.0~8.0
Enzymes work at high temperature with high reactivity
The "Thermostable Enzyme".
Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.
Features of Thermostable Enzyme
Highly stable and durable for long term use
High reactivity at high temperature (70 - 100°C)
Resistance to organic solvent is expected
Special instruments to inhibit bacterial growth are not required.
Reaction speficity is much higher compared with chemical reaction
Speed of reaction can be accelerated
Activity measurement
Reaction mix (50 mM Sodium acetate buffer (pH 4.0), 1 mM p-nitrophenyl-di-N-?-chitobioside (pNP-chitobioside) and appropriate amount of the enzyme) was incubated at 85 oC and A420 was measured after the addition of two volumes of 0.25 M Na2CO3. One unit is defined as the amount of enzyme producing 1 ?mol of p-nitrophenol per 1 minute using pNP-chitobioside as a substrate