Centrifugal Mesh Plate (Compatible with 96well) 70μm, 1 pc.
Product#: FNK-R-CSC079-01
$74.55
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Centrifugal Mesh Plate (Compatible with 96well) 70μm
Cat. No. FNK-R-CSC079-01
Size: 1 pc
Description:
- 96-well compatible microplate mesh seal
- Can process multiple samples efficiently
- Prevent contamination per well
- Unit cost of experiment (filtration cost) can be reduced
POINT
High-throughput multiple sample processing. It can be handled for automation.
Usage
Comparison before and after filtration
Performance evaluation of 96-well centrifugal filtration plate
Evaluation method:
Set a centrifugal filtration plate on a conventional 96-well round bottom plate and pass cells trhough filter → centrifugation
Evaluation point: PResence or absense of contamination after plate centrifugation
Method (experimental condition):
Presence or absence of contamination
HEK293T not expressing GFP and HEK293T expressing GFP (human fetal kidney epithelial cell) are filtered in adjacent wells and judged by flow cytometry
Operation:
1. Cells were stripped with trypsin/EDTA and centrifuged.
2. Cells were washed in PBS.
3. Cells were diluted with PBS to make about 2 x 104 cells/200 μL.
4. PI was added to make a final concentration of 0.25 μg/mL.
5. A centrifugal filtration plate was set on a 96-well plate.
6. Cells were added to wells in 200 μL.
7. The plate was centrifuged at 300 g x 30 s.
8. It was measured with flow cytometer (Guava easyCyte 8HT).
*Measurement was made only within the red flame
Operation conditions:
Results:
Summary of verification results:
- After centrifugation, there was no contamination in another well.
- This time, it was centrifuged at 300 g x 30 seconds, and all the suspension had fallen to the bottom.
