FluxMPS™ Ham's F-12 Nutrient Mixture, Coon's Modification: 2X Liquid
FluxMPS™ Ham's F-12 Nutrient Mixture, Coon's Modification: 2X Liquid
Double-strength (2X) Coon's modified Ham's F-12 — 4.0 g/L glucose in the concentrate, 2.0 g/L at 1X — for hybrid-cell culture, FRTL-5 and other rat thyroid cells, hormone-response and iodide-transport research and agarose culture, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a sodium bicarbonate buffer, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.
- 2X concentrate: mix with an equal volume of a compatible solution or matrix for a final 1X medium
- Coon's modification: about twice the amino acids and sodium pyruvate of standard F-12, plus ascorbic acid and an adjusted salt composition
- Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
- 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
- Endotoxin < 0.05 EU/mL (USP <85> BET)
- Formulation: [+] 4.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red; [−] HEPES
- Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
- D-Glucose4,000 mg/L
- L-Glutamine584 mg/L
- Sodium Pyruvate440 mg/L
- BufferNaHCO₃ 5,352 mg/L
- Phenol Red2.48 mg/L
- AppearanceRed to Bright Pink
- pH7.4 (± 0.04)
- Endotoxin< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Format2X liquid, 500 mL
Engineered where standard media fails
Conventional Ham's F-12 is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ Ham's F-12 keeps Ham's formulation and removes that failure mode.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.
Total metabolic control
Defined 4,000 mg/L D-glucose, 584 mg/L L-glutamine and 440 mg/L sodium pyruvate in the 2X (half that at 1X).
Ultrapure-grade water
Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.
Low background for imaging
Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing.
Matrix-ready 2X strength
50 components at double strength, so agar, hydrogels, supplements or treatment solutions can be added 1:1 without diluting the medium below 1X.
Customization on demand
pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-F12HCM2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X Ham's F-12 polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.
-
01
0.1 µmPre-filtration I
Removes large particulates and aggregates and extends the life of the downstream filters.
-
02
0.04 µmPre-filtration II
Retains fine particulates, bacteria and mycoplasma.
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03
0.1 µmSterile-filtration I
Second-pass redundancy that catches anything shed or bypassed upstream.
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04
0.04 µmSterile-filtration II — Final Polish
Final nano-polish immediately before ISO Class 5 aseptic fill.
Performance vs. conventional media
By particulate count, FluxMPS™ Ham's F-12 is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.
© Diagnocine® — DCP-F12HCM2X
Where FluxMPS™ Coon's F-12 fits
Ham’s F-12 Nutrient Mixture, Coon’s Modification, is a modified basal medium for in vitro mammalian-cell research. Relative to standard Ham’s F-12, it contains approximately twice the amino-acid and sodium-pyruvate concentrations, includes ascorbic acid, and has an adjusted salt composition.[2] Originally developed for hybrid-cell culture, Coon’s modified F-12 is also used, with appropriate supplements, for rat thyroid cell lines such as FRTL-5.[1,3] It provides a basal nutrient environment for laboratory studies of cell growth, hormone responsiveness, and differentiated thyroid-cell functions.
Ham’s F-12 Nutrient Mixture, Coon’s Modification, 2X, is a double-strength version of the corresponding 1X Coon’s formulation, intended for laboratory workflows that dilute the basal medium during final preparation. Its purpose is dilution compensation, following the established use of 2X media in equal-volume mixtures with aqueous agar or agarose preparations.[4] Mixing one volume of 2X medium with one volume of a compatible solution that contributes none of its basal ingredients gives those ingredients at their intended 1X concentrations. The published agar/agarose methods establish this dilution principle, not the compatibility or performance of a specific 2X Coon’s product.
Important distinction: Ordinary 1X Coon’s modification already has approximately double the amino acids and pyruvate of standard F-12; that does not make it a 2X medium. “2X Coon’s” means twice the corresponding Coon’s formulation, not simply another name for standard-strength Coon’s modified F-12.
It is useful for:
- Hybrid-cell culture research
- Rat thyroid-cell culture
- Hormone-response and cell-signaling research
- Iodide-transport and thyroid-function research
- Cell-based chemical-response studies
- Chondrogenic differentiation research
- Semisolid or agarose culture development
Automated Bioreactors & Robotics
For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.
- Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
- Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
- Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.
Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.
FRTL-5 & Rat Thyroid Cells
The basal medium for hormone-dependent FRTL-5 thyroid epithelial cells when supplemented with serum and a defined hormone mixture.[3]
Hybrid-Cell Culture
Coon's modification was developed for the formation and growth of viable somatic cell hybrids.[1]
Hormone Response & Iodide Transport
A basal environment for studying hormone responsiveness, cell signaling, iodide uptake and other differentiated thyroid-cell functions.[3]
Chondrogenic & Agarose Culture
A double-strength base for agarose and other semisolid preparations, including chondrogenic differentiation research.[4]
Organ-on-a-Chip & MPS
Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]
Microscopy & Optical Sensing
Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors.
Specifications & quality control
Release specifications for DCP-F12HCM2X. Lot-specific values are reported on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | [+] 4.0 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red; [−] HEPES |
| Concentration | 2X (dilute 1:1 to 1X) |
| Appearance | Red to Bright Pink |
| pH | 7.4 (± 0.04) USP <791> |
| Osmolality | Measured per lot; reported on CoA USP <785> |
| D-Glucose | 4,000 mg/L (4.0 g/L; 2.0 g/L at 1X) |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | 440 mg/L |
| Sodium Bicarbonate | 5,352 mg/L |
| Phenol Red | 2.48 mg/L |
| HEPES | Not included |
| Parameter | Specification |
|---|---|
| Endotoxin | < 0.05 EU/mL USP <85> BET |
| Sterility | No bacterial or fungal growth after 14 days of incubation USP <71> |
| Mycoplasma | 0.04 µm barrier filtration USP <63> equiv. |
| Particulate ≥10 µm | Meets limits USP <788> M2 |
| Particulate ≥25 µm | Meets limits USP <788> M2 |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) ISO |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8 °C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months; use before the expiry date on the product label |
| Shipping condition | Ice Pack |
| CO₂ requirement | CO₂-controlled incubator after dilution to 1X; sodium bicarbonate 5,352 mg/L in the 2X, 2,676 mg/L at 1X (classically equilibrated with 10% CO₂; many protocols use 5%) |
| Parameter | Specification |
|---|---|
| Raw material grade | High-purity, cell-culture-tested components |
| Traceability | Lot-traceable; CoA per lot |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
DCP-F12HCM2X is 2X Coon's modified Ham's F-12 with 4.0 g/L glucose, L-glutamine, sodium pyruvate, sodium bicarbonate and Phenol Red. It does not contain HEPES. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 50 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 330.000 |
| Copper sulphate pentahydrate | 7758-99-8 | 0.0050 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 264.080 |
| Ferrous sulphate heptahydrate | 7782-63-0 | 1.668 |
| Magnesium chloride hexahydrate | 7791-18-6 | 212.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 101.600 |
| Potassium chloride | 7447-40-7 | 610.000 |
| Potassium dihydrogen phosphate | 7778-77-0 | 136.000 |
| Sodium bicarbonate | 144-55-8 | 5352.000 |
| Sodium chloride | 7647-14-5 | 15060.000 |
| Zinc sulphate heptahydrate | 7446-20-0 | 0.288 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 32.000 |
| L-Alanine | 56-41-7 | 36.000 |
| L-Arginine hydrochloride | 1119-34-2 | 844.000 |
| L-Asparagine anhydrous | 70-47-3 | 60.000 |
| L-Aspartic acid | 56-84-8 | 52.000 |
| L-Cysteine hydrochloride | 52-89-1 | 140.520 |
| L-Glutamic acid | 56-86-0 | 60.000 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 84.000 |
| L-Isoleucine | 73-32-5 | 15.600 |
| L-Leucine | 61-90-5 | 52.400 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 18.000 |
| L-Phenylalanine | 63-91-2 | 20.000 |
| L-Proline | 147-85-3 | 140.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 47.600 |
| L-Tryptophan | 73-22-3 | 8.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 31.720 |
| L-Valine | 72-18-4 | 46.800 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.0146 |
| Choline chloride | 67-48-1 | 27.920 |
| D-Ca-Pantothenate | 137-08-6 | 0.960 |
| Folic acid | 59-30-3 | 2.640 |
| L-Ascorbic acid | 50-81-7 | 90.000 |
| Nicotinamide | 98-92-0 | 0.080 |
| Pyridoxine hydrochloride | 58-56-0 | 0.120 |
| Riboflavin | 83-88-5 | 0.080 |
| Thiamine hydrochloride | 67-03-8 | 0.680 |
| Vitamin B12 | 68-19-9 | 2.720 |
| i-Inositol | 87-89-8 | 36.040 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4000.000 |
| Hypoxanthine sodium salt | 45738-97-4 | 8.080 |
| Linoleic acid | 60-33-3 | 0.180 |
| Lipoic acid | 1077-28-7 | 0.412 |
| Phenol Red | 34487-61-1 | 2.480 |
| Putrescine dihydrochloride | 333-93-7 | 0.600 |
| Sodium pyruvate | 113-24-6 | 440.000 |
| Thymidine | 50-89-5 | 1.400 |
Manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.
ISO 13485:2016 QMS
Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.
ISO Class 5 Fill & Finish
Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.
Micro-Batch Precision
Small, tightly controlled batches for consistent composition and pH from lot to lot.
Endotoxin — USP <85> BET
Released at < 0.05 EU/mL.
Particulate — USP <788> Method 2
Subvisible particles ≥10 µm and ≥25 µm within compendial limits.
Osmolality — USP <785>
Measured per lot and reported on the CoA.
Documentation / CoA
Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.
How DCP-F12HCM2X compares
Same Ham's F-12 chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.
| Parameter | DCP-F12HCM2X (FluxMPS™) | Conventional Coon's F-12, 1X (0.22 µm filtered) | Conventional 2X Ham's F-12 (0.22 µm filtered) |
|---|---|---|---|
| Formulation | 2X Coon's modified F-12; complete formulation | 1X Coon's F-12, ready to use | Double-strength, for semisolid culture |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin specification | < 0.05 EU/mL | Often not specified at this level | Often not specified at this level |
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | Varies | Varies |
| ISO 13485:2016 manufacturing | check_circle | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about using DCP-F12HCM2X for semisolid, 3D, microfluidic and conventional culture.
Supporting literature
Peer-reviewed background on Coon's modified F-12, hybrid-cell and FRTL-5 thyroid culture, soft-agar culture, Phenol Red, organ-on-a-chip culture and mycoplasma control.
- Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus-produced viable hybrids. Proc Natl Acad Sci USA. 1969;62(3):852–859. doi:10.1073/pnas.62.3.852
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53(2):288–293. doi:10.1073/pnas.53.2.288
- Ambesi-Impiombato FS, Parks LA, Coon HG. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc Natl Acad Sci USA. 1980;77(6):3455–3459. doi:10.1073/pnas.77.6.3455
- Borowicz S, Van Scoyk M, Avasarala S, et al. The soft agar colony formation assay. J Vis Exp. 2014;(92):e51998. doi:10.3791/51998
- Barnes D, Sato G. Methods for growth of cultured cells in serum-free medium. Anal Biochem. 1980;102(2):255–270. doi:10.1016/0003-2697(80)90151-7
- Morton HJ. A survey of commercially available tissue culture media. In Vitro. 1970;6(2):89–108. doi:10.1007/BF02616111
- Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
- Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029
