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Cat Interleukin 8, IL-8 ELISA Kit
Product#: BTL-E0052Cat
$700.00
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Cat Interleukin 8, IL-8 ELISA Kit
Cat. No#: BTL-E0052Cat
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 1.48ng/L
Detection range: 2-600ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Cat
Product overview
Full product name
Interleukin 8
SKU#.: ? BTL- |
E0052Cat |
Assay type |
Sandwich |
Size |
1.48ng/L |
Detection range |
2-600ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Cat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus (By similarity). |
UniProt accession |
11,165 |
GeneID |
493836 |
Synonyms |
Chemokine;C-X-C motif chemokine 8;C-X-C motif ligand 8;IL 8;IL8;IL-8;Interleukin-8 |
Gene names |
CXCL8 |
Research area |
Cardiovascular; Immunology |
Target protein |
IL-8 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0052Cat are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 320ng/L | 1.912 | 1.86 | 1.818 |
| 1.807 | |||
| 160ng/L | 1.078 | 1.117 | 1.076 |
| 1.157 | |||
| 80ng/L | 0.445 | 0.496 | 0.454 |
| 0.547 | |||
| 40ng/L | 0.222 | 0.266 | 0.224 |
| 0.31 | |||
| 20ng/L | 0.163 | 0.134 | 0.093 |
| 0.105 | |||
| 0ng/L | 0.047 | 0.041 | 0 |
| 0.035 |
Precision
We measured random samples of E0052Cat within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 91.12 | 4.7 | 5.15 |
| Intra-Assay | 2 | 18 | 140.81 | 7.02 | 4.98 |
| Intra-Assay | 3 | 18 | 205.67 | 6.74 | 3.28 |
