Ready to use
Cat Interferon Beta, IFN-beta ELISA Kit
Cat. No#: BTL-E0043Cat
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 1.22ng/L
Detection range: 2-700ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Cat
Product overview
Full product name
Interferon beta
SKU#.: ? BTL- |
E0043Cat |
Assay type |
Sandwich |
Size |
1.22ng/L |
Detection range |
2-700ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Cat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Has antiviral, antibacterial and anticancer activities. |
UniProt accession |
22,188 |
GeneID |
493849 |
Synonyms |
Fibroblast interferon;IFNB;IFN-beta;Interferon beta |
Gene names |
IFNB1 |
Research area |
Cardiovascular; Immunology; Cancer |
Target protein |
IFN-beta |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0043Cat are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 400ng/L | 2.097 | 2.096 | 2.067 |
| 2.095 | |||
| 200ng/L | 1.519 | 1.587 | 1.558 |
| 1.655 | |||
| 100ng/L | 1.144 | 1.122 | 1.093 |
| 1.101 | |||
| 50ng/L | 0.69 | 0.684 | 0.655 |
| 0.677 | |||
| 25ng/L | 0.459 | 0.466 | 0.437 |
| 0.474 | |||
| 0ng/L | 0.027 | 0.029 | 0 |
| 0.031 |
Precision
We measured random samples of E0043Cat within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 170.71 | 11.57 | 6.78 |
| Intra-Assay | 2 | 18 | 69.31 | 3.23 | 4.65 |
| Intra-Assay | 3 | 18 | 26.74 | 1.86 | 6.94 |
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