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Cat Interleukin 1 Beta, IL-1B ELISA Kit
Product#: BTL-E0055Cat
$700.00
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Cat Interleukin 1 Beta, IL-1B ELISA Kit
Cat. No#: BTL-E0055Cat
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 1.19pg/ml
Detection range: 2-600pg/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Cat
Product overview
Full product name
Interleukin 1 Beta
SKU#.: ? BTL- |
E0055Cat |
Assay type |
Sandwich |
Size |
1.19pg/ml |
Detection range |
2-600pg/ml |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Cat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. |
UniProt accession |
30,362 |
GeneID |
768274 |
Synonyms |
IL 1B;IL-1 beta;il1b;IL-1B;Interleukin-1 beta |
Gene names |
IL1B |
Research area |
Immunology; Cardiovascular |
Target protein |
IL-1B |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0055Cat are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 320pg/ml | 2.317 | 2.337 | 2.271 |
| 2.356 | |||
| 160pg/ml | 1.429 | 1.359 | 1.294 |
| 1.29 | |||
| 80pg/ml | 0.898 | 0.859 | 0.794 |
| 0.821 | |||
| 40pg/ml | 0.412 | 0.476 | 0.411 |
| 0.54 | |||
| 20pg/ml | 0.276 | 0.262 | 0.197 |
| 0.249 | |||
| 0pg/ml | 0.066 | 0.066 | 0 |
| 0.065 |
Precision
We measured random samples of E0055Cat within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 111.73 | 6.31 | 5.65 |
| Intra-Assay | 2 | 18 | 195.03 | 6.98 | 3.58 |
| Intra-Assay | 3 | 18 | 32.12 | 2.47 | 7.68 |
