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Cat Interleukin 1 Alpha, IL-1A ELISA Kit
Product#: BTL-E0054Cat
$700.00
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Cat Interleukin 1 Alpha, IL-1A ELISA Kit
Cat. No#: BTL-E0054Cat
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.32ng/L
Detection range: 0.5-200ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Cat
Product overview
Full product name
Interleukin 1 Alpha
SKU#.: ? BTL- |
E0054Cat |
Assay type |
Sandwich |
Size |
0.32ng/L |
Detection range |
0.5-200ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Cat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells (By similarity). |
UniProt accession |
30,798 |
GeneID |
493944 |
Synonyms |
IL 1A;IL-1 alpha;IL1A;IL-1A;Interleukin-1 alpha;L1A |
Gene names |
IL1A |
Research area |
Immunology |
Target protein |
IL-1A |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0054Cat are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 120ng/L | 2.686 | 2.664 | 2.607 |
| 2.642 | |||
| 60ng/L | 1.472 | 1.496 | 1.439 |
| 1.519 | |||
| 30ng/L | 0.799 | 0.767 | 0.711 |
| 0.736 | |||
| 15ng/L | 0.466 | 0.422 | 0.365 |
| 0.379 | |||
| 7.5ng/L | 0.23 | 0.222 | 0.166 |
| 0.215 | |||
| 0ng/L | 0.048 | 0.057 | 0 |
| 0.066 |
Precision
We measured random samples of E0054Cat within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 42.86 | 1.7 | 3.97 |
| Intra-Assay | 2 | 18 | 60.3 | 1.63 | 2.7 |
| Intra-Assay | 3 | 18 | 22.45 | 1.15 | 5.14 |