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Cat Interleukin 1 Alpha, IL-1A ELISA Kit

Product#: BTL-E0054Cat
$700.00
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Cat Interleukin 1 Alpha, IL-1A ELISA Kit



Cat. No#: BTL-E0054Cat



*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com



Product Summary

Size: ,96T

Sensitivity: 0.32ng/L

Detection range: 0.5-200ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Cat





Product overview

Full product name

Interleukin 1 Alpha

SKU#.: ? BTL-

E0054Cat

Assay type

Sandwich

Size

0.32ng/L

Detection range

0.5-200ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Cat

Storage

2-8ºC

Assay time

1h 30m

Background

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells (By similarity).

UniProt accession

30,798

GeneID

493944

Synonyms

IL 1A;IL-1 alpha;IL1A;IL-1A;Interleukin-1 alpha;L1A

Gene names

IL1A

Research area

Immunology

Target protein

IL-1A


Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1


Protocol data

These standard curves of E0054Cat are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/L 2.686 2.664 2.607
2.642
60ng/L 1.472 1.496 1.439
1.519
30ng/L 0.799 0.767 0.711
0.736
15ng/L 0.466 0.422 0.365
0.379
7.5ng/L 0.23 0.222 0.166
0.215
0ng/L 0.048 0.057 0
0.066


Precision

We measured random samples of E0054Cat within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 42.86 1.7 3.97
Intra-Assay 2 18 60.3 1.63 2.7
Intra-Assay 3 18 22.45 1.15 5.14


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